Paclitaxel is a very important anticancer drug commonly called Taxol®.
We know from previous work (Hoffman, et al., 1998) that Taxol exists in filbert, Corylus avellana L. material. Quantification of Taxol in filbert plant material is painstaking and hitherto was accomplished by rapidly processing single batches in a complicated procedure...
Experiments were conducted to determine the influence of cultural management practices on weed growth in nursery containers. Experiments 1 and 2 evaluated the effects of dolomitic lime rate and application method on substrate pH, creeping woodsorrel (Oxalis corniculata L.) establishment in containers, and growth of azalea (Rhododendron ‘Rosebud’) and pieris...
Four aspects of factors influencing the accuracy of nondestructive chlorophyll (Chl) and nitrogen (N) measurement in fresh leaves were studied: (1) optimum wavelength (OW) identification; (2) indices development and evaluation; (3) influence of leaf properties; and (4) influence of meter parameters and sampling technique. Results were used to develop indices...
The effect of incorporated sawdust, sawdust mulch and nitrogen (N) fertilizer rate on soil nutrients and other properties, N uptake, and plant growth in a young blueberry planting on a silt loam soil type was studied in 2004 and 2005. ‘Elliott’ highbush blueberry plants were established on raised beds that...
Research was conducted in the field and greenhouse to determine if a
kaolinite-based particle film, Surround®, would reduce colonization and early season
feeding damage in cucurbits by the Western Striped Cucumber Beetle, Acalymma
trivitattum (Mannerheim). Greenhouse studies were designed to determine effect of
particle film on cucumber dry weight production...
Experiments focusing on plant growth regulators’ concentrations and combination, mineral salt formulations and TDZ pretreatment formations were conducted to optimize in vitro shoot regeneration from leaf and petiole explants of 'Marion' blackberry. Optimum shoot formation was obtained when stock plants were incubated in TDZ pretreatment medium for three weeks before...
Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. In the first study, 12 robust Simple Sequence Repeat (SSR) markers were developed from two microsatellite-enriched libraries of...
It is estimated that 50-75% of Oregon vineyards cultivate at least every other alley in order to 1) reduce water stress 2) increase vineyard canopy temperatures and 3) increase nutrient availability. Because many vineyards are situated on steep hillsides, frequent tillage could result in increased soil erosion, decreased soil quality...
Solanum species differ in their degree of frost tolerance and cold acclimation capacity. Cultivated potato species of Solanum tuberosum L. are frost-sensitive, incapable of cold acclimation, and have a maximum freezing tolerance of –3°C. Solanum commersonii Dun is frost-tolerant and can survive to –5ºC pre-acclimation and –10ºC post-acclimation. Breeding attempts...
In Pisum, the Np gene conditions two mitotic responses - to bruchid weevil oviposition on the pod and to reduced UV light. Oviposition by the weevil results in tumorous or neoplastic growth under the egg. Biochemically active compounds, called bruchins, were isolated from two bruchid insects (Doss et al, 2000)....
In mature Arabidopsis seeds, the testa (seed coat) is no longer a living tissue. Thus,
major sites of gene expression in imbibed seeds are the internal living tissues such
as the embryo and the endosperm. To elucidate the molecular mechanisms of seed
germination, the gene regulation in these two tissues...
Orchard floor management (OFM) practices can improve soil function and alter
the soil biological community. Soil function is dependent on soil forming materials and
on management practices but little is known about how OFM affect orchard soil quality,
fertilizer N uptake, or tree growth and production.
Research plots were located...
In recent years, there has been renewed interest in black raspberry (Rubus occidentalis L.) breeding. This has been spurred by an increase in black raspberry consumption due to studies that have shown them to be particularly high in anthocyanin content indicating high levels of antioxidants. Present cultivars are ill adapted...
A series of studies during six years examined vegetative growth and fruit yield of the 'Olympus' strawberry when grown with black polyethylene mulch and drip irrigation. In some studies, plants were grown with or without mulch and irrigated at low, medium, or high levels for one or two months in...
The hundred-year history of the hazelnut industry in the Pacific Northwest is threatened by eastern filbert blight (EFB) caused by the fungus Anisogramma anomala (Peck) E. Müller. Marker-assisted selection (MAS) has been extensively used for ‘Gasaway’ resistance in the hazelnut breeding program at Oregon State University. Concern over breakdown of...
White mold, caused by Sclerotinia sclerotiorum (Lib.) de Bary, results in severe losses in the production of common bean, Phaseolus vulgaris, especially snap bean varieties. Advanced backcross QTL analysis was used to identify quantitative trait loci for resistance to white mold in an interspecific cross of P. vulgaris and P....
A rootstock trial was established in 1997 at the OSU Woodhall vineyard, located in the Southern Willamette Valley, which included two experiments. The first experiment was a factorial of four varieties grafted on to 9 rootstocks, and also as ungrafted vines (a split-plot design). The second experiment was a randomized...
The effect of primocane management on flowering and fruiting patterns was studied in primocane-fruiting blackberry (Rubus subgenus Rubus, Watson) cultivars 'Prime-Jan'® and 'Prime-Jim'® over two years. Primocanes that were "soft-tipped" (upper 2-5 cm removed) at 1.0 m were compared to un-tipped canes. In both years, soft-tipped primocanes developed 2-3 fold...
Seed germination sensu stricto is defined as the physiological events before the radicle tip ruptures the covering tissues. The ability of the radicle to elongate (or germination potential) is observed in developing embryos prior to completion of seed maturation. When embryos at early developmental stages, such as globular, heart, torpedo...
Interspecific hybridization was initiated between wild P. acutifolius accession G40199 and P. vulgaris to introgress resistance to Acanthoscelides obtectus (bean seed weevil). F1 interspecific hybrids were recovered by embryo rescue and maintained until flowering. Mostly sterile hybrids were backcrossed twice to common bean cultivar ICA Pijao to recover sufficient fertility...
Root rot of sweet corn in western Oregon and Washington is a significant disease that
can reduce yield of intolerant cultivars of processed sweet corn by fifty percent. Root rot
is caused by a complex of soilborne organisms, including Drechslera sp., Phoma
terrestris, and Pythium arrhenomanes. Processors have adopted tolerant...
We have conducted a full-time program of vegetable crop research at the North Willamette Research and Extension Center (formerly the North Willamette Experiment Station) since 1976. The Center, a branch of both Oregon State University's Agricultural Experiment Station and its Extension Service, is just north of Aurora, a historic farming...
A regional analysis of the street tree problem has been undertaken in order to evolve a system of tree classification
which would be useful to city planners, arborists, and landscape architects who have the responsibility of selecting trees to fulfill their design requirements for the city street. Three lines of...
Five-year- old 'Ananasnaya' hardy kiwifruit [Actinidia arguta (Sieb. & Zucc.) Miq.] vines in a commercial vineyard were subjected to flower/fruit thinning in 1999. Treatments of thinning flowers before bloom (BB; June 2 to 5) or fruit after set (AFS; - July 1 to 7) at three different seventies (15%, 30%...
Container grown pear trees were used to determine the effects of the following factors on the susceptibility to Phytophthora syringae: 1) tree nitrogen (N) status and foliar sprays of urea and copper-EDTA (CuEDTA); 2) dormancy and environmental conditions; 3) wound and wound age; and 4) chemical and biological control measures....
Elite inbred sunflower (Helianthus annuus L.) lines were found to have short-lived embryo and seed covering dormancies. Seed dormancy of wild sunflowers (H. annuus, H. argophyllus and H. exilis) was found to be controlled primarily by the seed covering (seed coat and pericarp) and embryo dormancy was short-lived (four to...
Two studies were initiated in June of 1981 in order to gain greater understanding of the growth and fruiting habits of field grown strawberries in Oregon's Willamette Valley.
Yield component analysis of 'Benton', Oregon's leading cultivar and an advanced breeding selection, 'OR-US 4356' was conducted during the first (1981) and...
Size and color are important visual quantity attributes of the fruit of strawberry (Fmgaria x ananassa Duch.). Strawberry cultivars produce fruit of a characteristic size and color, the later being the result of accumulation of particular anthocyanins. This research sought to determine the basis for genotypic differences in fruit size...
This paper records a series of experiments conducted furing a one-year period to determine the effects of boron upon the growth and fruiting of Marshall and Corvallis strawberries, two of the leading commercial varieties in Oregon. The problem was approached in four ways: (1) applications of dry borax and borax...
Several plant hormones, including ethylene and jasmonates, are known to mediate responses against biotic and abiotic stress. The first and second objectives of this thesis were (i) to elucidate the role of ethylene in defense responses against Botrytis cinerea and specifically (ii) to determine the potential contribution of ethylene response...
Blackberry varieties were evaluated with modified atmosphere packaging, using films Cryovac D-940 and D-955 in 0° and 5°C storage. Moisture loss was kept extremely
low (less than 3%) in packaged fruits, but mold infection was excessively high because of high humidity accumulated inside the container. Blackberries selectively harvested
into 3...
To determine the effects of rate of nitrogen application and storage time on fruit quality of highbush blueberries (Vaccinium corymbosum, L.), five rates of ammonium sulfate fertilizer were soil-applied in 1984 and 1985 to cvs. Bluecrop and Jersey. Fruit was harvested up to three times during the 1985 season and...
Grape phylloxera, Daktulosp/iaira vitfoliae (Fitch) development was studied under field and laboratory conditions. The aphid-like insect feeds on Vitis vinfera L. roots producing swellings that decay and lead to vine decline. The only long-term economic solution for control of grape phylloxera (GP) is to plant vines grafted onto a resistant...
Pollen development is an important process in male flower development, the timing of which may be correlated with time of pollen shed in hazelnut (Corylus avellana L.). Early to very late blooming cultivars were identified and the relationship of microsporogenesis and microgametogenesis, and time of pollen shed were studied in...
New highly polymorphic simple sequence repeat (SSR) markers for European hazelnut (Corylus avellana L.). were developed from several sources. The markers were characterized, and segregation in a mapping population allowed to assign them to linkage groups. Their transferability across genera and species in the Betulaceae family was investigated. SSR markers...
The purpose of this research was to develop tools and germplasm for the production of tomatoes (Solanum lycopersicum L.) with high fruit phenolics without the use of genetic engineering. A candidate gene analysis was undertaken to identify genes regulating anthocyanin biosynthesis in tomato fruit (Aft, Abg, atv, and Purple Smudge)....
Vegetable production with a living mulch may reduce soil erosion and compaction, increase organic matter levels, and decrease the requirement for chemical inputs. Competition between the vegetable and mulch, however, has limited the development of successful living mulch systems to realize these benefits. In a field study, interference between pak...
Hop powdery mildew, caused by Podosphaera macularis Braun & Takamatsu (formerly Sphaerotheca macularis (Wallr. :Fr.) Lind, syn. S. humuli (DC.)Burrill) was not observed in Pacific Northwest hop yards until 1997, when it was discovered in Washington. Within one year, it had spread to Oregon and Idaho. This emerging disease caused...
North American cultivation of Ribes L. may expand as small fruit growers seek species to diversify horticultural crops. The Ribes industry was suppressed for decades out of fear that cultivated black currants and gooseberries would intensify the fungal disease white pine blister rust (WPBR) on five-needle pine (Pinus L. section...
Legume cover crops can serve as important sources of nitrogen (N) in sustainable agriculture and can be economically beneficial when fertilizer inputs are reduced without a yield reduction. Synchronizing N mineralization from organic materials with the needs of the subsequent crop is a challenge for organic growers.
Predicting plant available...
An alternative to traditional roofing, extensive green roofs are contained ecosystems consisting of a drainage layer, a thin media profile which is planted with hardy plant species. Extensive green roof plants must maintain multiple functions while growing in a highly aggregate media at a depth of equal to or less...
The effects of ¹⁵N-labeled fertilizer applied to mature summer-bearing red raspberry (Rubus idaeus L.) plants were measured over two years. Four nitrogen (N) treatments were applied singularly at 0, 40, or 80 kg·ha⁻¹ of N in early spring (budbreak), or split with 40 kg·ha⁻¹ of N (unlabeled) applied at budbreak...
Rubus seed characteristics; seed coat morphology, anatomy, germination requirements and dormancy were studied in order to define protocols for breaking seed dormancy. Morphological analysis of the seed coat (testa) using scanning electron microscopy (SEM) included 56 seed accessions of 10 subgenera preserved in the collections of the US Department of...
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic pathogen capable of causing white mold, a severe disease in common bean. White mold is of particular concern to the Oregon snap bean processing industry, where processors allow less than
3% incidence in harvested shipments. Breeding for white mold resistance in beans...
Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips...
This dissertation describes the application and development of molecular tools that have great potential for use in studying variation in strawberry germplasm. The first study evaluated 91 microsatellite (simple sequence repeat, SSR) markers for transferability in 22 Fragaria species and their utility in fingerprinting octoploid strawberries. Out of the transferable...
The advanced backcross-quatitative trait loci (AB-QTL) breeding method was employed on snap bean (OR 91G x PI433251B) and dry bean (M0162 x PI433251B) interspecific populations to transfer resistance QTL from resistant donor parent Phaseolus coccineus into P. vulgaris. For OR 91G x PI433251B, analyses produced nine linkage groups corresponding to...