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Filtering by: Subject Spermatogenesis Academic Affiliation Animal Sciences

Flow cytometric evaluation of spermatozoan nuclear chromatin structure and viability

Creator:

Ballachey, Brenda Elizabeth

Abstract:

I. Frozen bovine semen samples were measured by flow cytometry (FCM) to determine if FCM analyses could be useful for sperm quality evaluation and prediction of bull fertility. Fluorescent dyes utilized were acridine orange, to measure sperm nuclear chromatin heterogeneity by quantifying susceptibility of the chromatin to denaturation, and rhodamine...

Resource Type:

Dissertation