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Filtering by: Creator Brevig, Rod E. Remove constraint Creator: Brevig, Rod E. Academic Affiliation Biochemistry and Biophysics Remove constraint Academic Affiliation: Biochemistry and Biophysics Degree Name Doctor of Philosophy (Ph.D.) Remove constraint Degree Name: Doctor of Philosophy (Ph.D.)

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  • Dietary organosulfur and organoselenium compounds as HDAC inhibitors

    Creator:
    Nian, Hui
    Abstract:
    Histone deacetylase (HDAC) inhibitors have the potential to de-repress epigenetically silenced genes in cancer cells, leading to cell cycle arrest and apoptosis. Dietary HDAC inhibitors derived from natural phytochemicals are promising anticancer agents. In this thesis, metabolites from natural organosulfur and organoselenium compounds, i.e. allyl mercaptan (AM), β-methylselenopyruvate (MSP) and...
    Resource Type:
    Dissertation
    Full Text:
      intestinal alkaline phosphatase,  sodium hydrogen exchanger NHE3,  the adherens junction protein E‐cadherin

  • Hydrodynamic properties of homogeneous double-stranded DNA

    Creator:
    Kovacic, Roger Timothy
    Resource Type:
    Dissertation
    Full Text:
    : HYDRODYNAMIC PROPERTIES OF HOMOGENEOUS DOUBLE-STRANDED DNA Abstract approved: Redacted for Privacy K. E. Van

  • Physico-chemical studies on the structure of eukaryotic chromatin

    Creator:
    Sahasrabuddhe, Chintaman Gopal
    Abstract:
    It has been shown that about 50 % of the whole chromatin DNA exists in a very compact form in calfthymus chromatin.These compact fragments can be extracted from the whole chromatin as nuclease resistant fragments by limited nuclease digestion. These fragments involve four out of five histones in folding the...
    Resource Type:
    Dissertation
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    B) Chromatin from calf thymus C) PS-fragments D) TPS-fragments & E) DNA. 28 34 47 48 Absorption

  • In vitro transcription in the yeast: Saccharomyces cerevisiae

    Creator:
    Ide, Gregory James
    Abstract:
    The structure and transcriptional activity of intranuclear and isolated chromatin from logarithmically growing yeast cells has been compared to chromatin from cells which have entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern and a 160 by repeat size when digested with staphlococcal nuclease....
    Resource Type:
    Dissertation
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    CEREVISIAE Abstract approved: Redacted for Privacy K. E. Van Holde The structure and transcriptional

  • Nucleosome instabilities in physiological salt

    Creator:
    Yager, Thomas D.
    Abstract:
    Best scan available for figures. Some figures are high gloss photos, others are black and white photocopies.
    Resource Type:
    Dissertation
    Full Text:
    , criticism and advice: G. Ackers, K. Ahern, G. Bailey, E.M. Bradbury, D. Brooker, J. Davie, H. Eisenberg, E

  • Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

    Creator:
    Dong, Feng
    Abstract:
    By using immobilized trypsin, I have been able to prepare well-defined, stable trypsinized nucleosomes. The difficulties of lacking of control in the extent of trypsinization, which were encountered in previous studies with the use of free trypsin, have been eliminated. The nucleosomes and oligonucleosomes prepared by immobilized trypsin are suitable...
    Resource Type:
    Dissertation
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    . Kensal E. van Holde By using immobilized trypsin, I have been able to prepare well-defined, stable

  • Physical structure and reconstitution of chromatin core particles

    Creator:
    Tatchell, Kelly George
    Abstract:
    The nucleosome appears to be the basic structural unit of the eukaryotic chromosome. The usual nucleosome preparation, isolated from a micrococcal nuclease digest of nuclei, is heterogenous in DNA length and protein composition with some particles containing spacer DNA and associated protein. This heterogeneity complicated physical and reconstitution studies. As...
    Resource Type:
    Dissertation
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    PARTICLES Abstract approved: Redacted for Privacy K. E. Van Ho lde The nucleosome appears to be the basic

  • The mechanism of interaction of the linker histone with DNA and nucleosomes

    Creator:
    Ellen, Thomas Patrick
    Abstract:
    This dissertation examines the interaction of the linker histone with DNA and with nucleosomes. The first goal of the project was to characterize the interaction of the linker histone with DNA. Three factors previously reported to influence the linker histone's interaction with DNA were examined: ratio of linker histone to...
    Resource Type:
    Dissertation
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    Dr. Kensal E. van Holde for his support of this research and for the encouragement, patience, and

  • The binding of ethidium bromide to chromatin : model for carcinogen interactions

    Creator:
    McMurray, Cynthia T.
    Abstract:
    Five forms of chromatin, which represent the in vivo folded forms of nucleic acid, were isolated and used as the binding substrate for model intercalating compound, ethidium bromide. For all forms of chromatin, the affinity, location and structural effects of binding were examined. On the level of the nucleosome, the...
    Resource Type:
    Dissertation
    Full Text:
    19 2. Chicken Erythrocyte DNA 19 C. 31P-NMR Studies 19 D. H 1 Nuclear Magnetic Resonance 20 E

  • Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

    Creator:
    Sargent, Roy Geoffrey
    Abstract:
    Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic for DNA synthesis in both intact cells and cell-free replication systems. Almost certainly, such mutagenesis involves competition between correctly and incorrectly base-paired precursors at replication sites. However, there are certain differences between the intact cell and cell-free systems that do not always allow...
    Resource Type:
    Dissertation
    Full Text:
    coli (E. coli) ribonucleotide reductase. During T4 nrd+ infections, BrUdR mutagenesis preferentially