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  • A role for ceramide accumulation in age-related cardiac mitochondrial dysfunction

    Creator:
    Monette, Jeffrey S.
    Abstract:
    Ceramides are a group of lipids in the sphingolipid family that are potent cell signaling molecules. Elevations in ceramide levels above the norm generally lead to apoptosis. Evidence from in vitro studies suggests that accumulation of ceramides within mitochondria leads to dysfunction of the mitochondria. This includes inhibition of the...
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    Dissertation
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    S. Monette for the degree of Doctor of Philosophy in Biochemistry and Biophysics presented on

  • Glutamate dehydrogenase : I.Flourescence polarization studies of the self-association. II.Flourescence and circular dichroism studies of the heterotropic interactions

    Creator:
    Malencik, Dean Anthony
    Abstract:
    Fluorescence polarization measurements on beef liver glutamate dehydrogenase conjugated with pyrene butyric acid demonstrate that the association of the enzyme is adequately described by a reversible indefinite association with a single equilibrium constant. The data obtained with protein concentrations up to 0.7 mg/ml in 0.05 M potassium phosphate, pH 7....
    Resource Type:
    Dissertation
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    privacy S. R. Anderson Fluorescence polarization measurments on beef liver glutamate dehydrogenase

  • Control of p53 tumor suppressor and peroxiredoxin activity through shifts incellular redox balance

    Creator:
    Stoner, Christopher S.
    Abstract:
    Aerobic organisms have evolved many sensory mechanisms that allow response to oxidants in the environment. One area of interest is the relationship between the activity of the tumor suppressor protein p53 and the redox state of thioredoxin. Human p53 activity is severely compromised in budding yeast lacking thioredoxin reductase. Evidence...
    Resource Type:
    Dissertation
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    THE DISSERTATION OF Christopher S. Stoner for the degree of Doctor of Philosophy in

  • Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous system of Manduca sexta

    Creator:
    Albin, Edward Elmo
    Abstract:
    Adenosine 3', 5' -cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3', 5' -cyclic monophosphate (cyclic GMP, cGMP) were quantitated in the central nervous system (CNS) of the insect Manduca sexta by competitive protein binding techniques. The M. sexta CNS was found to contain a strikingly high level of cGMP, about...
    Resource Type:
    Dissertation
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    cyclic nucleotide- stimulable protein kinases (ATP: protein phosphotransferase, EC 2. 7. 1. 37), a

  • Alternatives to the DNA precursor-synthesizing enzyme complex hypothesis

    Creator:
    Weitz, Stephen L.
    Abstract:
    Investigators have been studying an aggregate containing enzymes of deoxyribonucleoside triphosphate biosynthesis in T4-phage infected bacteria. They suggest that it behaves as an organized complex, efficiently channeling DNA precursors to the replication apparatus. Previous work has suggested that about 10 enzyme activities remain associated through several fractionation steps. This multi-enzyme...
    Resource Type:
    Dissertation
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    reported to sediment through sucrose gradients at 15-20 S. Also, individual activities are kinetically

  • In vivo detoxification of acetic acid in Saccharomyces cerevisiae

    Creator:
    Ding, Jun
    Abstract:
    The studies described in this thesis were motivated by ongoing efforts to develop lignocellulosic biomass as an efficient and practical source of renewable energy. Enormous problems complicate these efforts to reduce reliance on greenhouse gas-generating fossil fuels. Simply obtaining the fermentable sugars available in the cellulose and hemicellulose components of...
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    Dissertation
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    . S. cerevisiae has a long and rich history of use in food and beverage production, e.g., baking

  • In vivo Reactions of Tetrazines Incorporated through Genetic Code Expansion

    Creator:
    Blizzard, Robert J.
    Abstract:
    The inverse electron demand Diels Alder reaction between tetrazines and strained alkenes is an exceptionally useful tool in functionalizing to biomolecules since it is orthogonal to the chemistry of most living systems and have exceptionally high rate constants. In particular reactions between strained trans-cyclooctenes (sTCO) and tetrazines can achieve second...
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    Dissertation
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    Escherichia coli, the tetrazine containing amino acid, 4-(6-methyl-s- tetrazin-3-yl)phenylalanine (Tet-v2.0

  • Protein methylation at sites of blood vessel injury

    Creator:
    Weber, Darin J.
    Abstract:
    Blood vessel injury was found to release intracellular pools of protein D-aspartyl/L-isoaspartyl carboxyl methyltransferase (PIMT) into the extracellular milieu, where it became trapped. Trapped PIMT was able to utilize radiolabeled S-adenosyl-L-methionine (AdoMet) introduced into the circulation to methylate blood vessel proteins containing altered aspartyl residues specifically at the site of...
    Resource Type:
    Dissertation
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    radiolabeled S-adenosyl-L-methionine (Ado Met) introduced into the circulation to methylate blood vessel

  • Trans-cis isomerization of geraniol and geranyl phosphate by cell-free enzymes from higher plants

    Creator:
    Shine, Ward Edward
    Abstract:
    Neryl pyrophosphate, rather than geranyl pyrophosphate, is believed to be the immediate biological precursor of the cyclohexanoid monoterpenes because the cis-2, 3-double bond readily permits cyclization. Biosynthesis of neryl pyrophosphate could occur by either of two principal mechanisms: direct cis condensation of dimethylallyl pyrophosphate with isopentenyl pyrophosphate or trans-cis isomerization...
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    Dissertation
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    Materials and Chemicals 12 Substrate s 14 Geraniol- 14C 14 Gerany1-14C Phosphate 15 Unlabelled Geraniol and

  • Toxicity and in vitro metabolism of Senecio jacobaea pyrrolizidine alkaloids in the rat and mouse

    Creator:
    Ramsdell, Howard Scott
    Abstract:
    Senecio jacobaea (tansy ragwort) is a common weed in the Pacific Northwest that contains toxic pyrrolizidine alkaloids (PAs). Jacobine, the major PA in Senecio jacobaea, was incubated with rat liver microsomes and metabolites were isolated by high performance liquid chromatography (HPLC) and identified by mass spectrometric analysis. Metabolites included a...
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    Dissertation
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    AN ABSTRACT OF THE THESIS OF Howard S. Ramsdell for