Index Catalog // ScholarsArchive@OSU

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

Creator:: Shen, Rongkun
Abstract:: The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
Resource Type:: Dissertation
Full Text:: , especially Linda J. Wheeler (Benson) and Dr. Indira Rajagopal for their kind technical advice and support

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Creator:: Bestwick, Richard Keith
Abstract:: This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Resource Type:: Dissertation
Full Text:: research. Finally I would like to thank Linda, my wife, who provided the support and purpose for

Structural Insights into Novel Sugar Phosphate Cyclases and Flavoenzymes

Creator:: Kean, Kelsey M.
Abstract:: The function of a protein is defined by its three-dimensional structure, and in understanding the three-dimensional structure of a protein, we gain an understanding of its function and mechanism. Protein structures, especially at high resolution, can provide detailed insights into many elements of enzyme function and catalysis – identifying residues...
Resource Type:: Dissertation
Full Text:: portion of NADP(H), we analyzed a suite of high resolution structures (~1.5 Å) in complex with

The effects of protein associations on pyrimidine deoxyribonucleotide biosynthesis

Creator:: McGaughey, Kathleen M.
Abstract:: The faithful replication of DNA depends on the appropriate balance of DNA precursors. From studies conducted in bacteriophage T4, models for deoxyribonucleotide biosynthesis producing pools appropriate for DNA replication have made it possible to understand more complex systems. A portion of that body of evidence supports the concept that deoxyribonucleotide...
Resource Type:: Dissertation
Full Text:: ............................ 52 H 2 Two dimensional gel for E. co/i thymidylate synthase releasing T4 proteins in 0.6 M NaCI

Trinitrophenylation of bovine muscle pyruvate kinase

Creator:: Johnson, Stephen Craig
Resource Type:: Dissertation
Full Text:: presented January 7, 1980 Typed by Linda M. Johnson for Stephen C. Johnson To Linda ACKNOWLEDGEMENTS

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation
Full Text:: . I also wish to thank Linda Wheeler and my fellow students in the Mathews' research group. One of my

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Creator:: Martomo, Stella A.
Abstract:: Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
Resource Type:: Dissertation
Full Text:: thanks also to members of the Mathews laboratory, Indira, Linda, Kate, Mary Lynn, Korakod, Shiwei and

Measuring protein metal binding via mass spectrometry : copper, zinc superoxide dismutase and amyotrophic lateral sclerosis

Creator:: Rhoads, Timothy W.
Abstract:: Amyotrophic lateral sclerosis (ALS) is a devastating disease characterized by the progressive degeneration of motor neurons. Dominantly-inherited mutations to the antioxidant enzyme Cu,Zn superoxide dismutase (SOD1) cause 3-6% of all ALS cases. The complete mechanism behind the toxicity of mutant SOD1 remains unclear, although significant evidence points to aberrant or...
Resource Type:: Dissertation
Full Text:: AUTHORS Linda DeNoyer (SpectrumSquare Associates) provided the analysis programs used for the mass

Conformational changes in histone IV as induced by salts and nucleoside triphosphates

Creator:: Wickett, Richard Randall
Abstract:: The addition of salts or nucleoside triphosphates to histone IV solutions induces conformational changes in the histone molecule. These conformational changes are investigated by measurement of the fluorescence anisotropy of the histone tyrosine emission, the circular dichroism (CD) of the peptide bond and of the tyrosine CD. Changes induced by...
Resource Type:: Dissertation
Full Text:: Blohm and Linda Haley for the preparation of histone IV. Special thanks are due to Dr. H. J. Li with

Organization of the T4 dNTP synthetase complex at DNA replication sites

Creator:: Kim, JuHyun
Abstract:: With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Resource Type:: Dissertation
Full Text:: Victor L. Hsu for their advice and time spent for my thesis. I would like to thank Linda Wheeler and Dr

ScholarsArchive@OSU

Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

Structural Insights into Novel Sugar Phosphate Cyclases and Flavoenzymes

The effects of protein associations on pyrimidine deoxyribonucleotide biosynthesis

Trinitrophenylation of bovine muscle pyruvate kinase

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Measuring protein metal binding via mass spectrometry : copper, zinc superoxide dismutase and amyotrophic lateral sclerosis

Conformational changes in histone IV as induced by salts and nucleoside triphosphates

Organization of the T4 dNTP synthetase complex at DNA replication sites

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