Index Catalog // ScholarsArchive@OSU

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Creator:: Thompson, Cynthia Sue
Abstract:: The effects of oligoribonucleotides of identical or similar sequences to a ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
Resource Type:: Dissertation
Full Text:: long, depending on the species. In E. coli, S8 is 129 amino acids long with a molecular weight of

Nucleic acid polymerases associated with neoplasms

Creator:: Kiessling, Ann Drue
Abstract:: RNA tumor viruses, such as avian myeloblastosis virus (AMV) and MC29 virus, cause malignant transformation of the cells they infect. Transformed cells are able to continually produce progeny virus particles. Evidence is given that RNA-dependent RNA polymerase is present in virus infected cells. RNA-dependent RNA polymerase isolated from AMV-infected myeloblasts...
Resource Type:: Dissertation
Full Text:: (Bonar and Beard, 1959). The nucleic acid is composed of several species: 1) a high molecular weight RNA

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Creator:: Jensen, Ole Norregaard
Abstract:: A novel protocol for the study of protein-nucleic acid interactions is presented and demonstrated to be feasible. The protocol combines photochemical crosslinking techniques and mass spectrometric methods into a new strategy for identifying protein domains or amino acid residues that are in close contact with nucleic acid in protein-nucleic acid...
Resource Type:: Dissertation
Full Text:: determination of intact, UV-crosslinked protein-nucleic acid complexes and for molecular weight determination

Intercalation on hydrocarbons in polynucleotides

Creator:: Craig, Albert Morrie
Abstract:: The binding of eleven polycyclic, aromatic hydrocarbons to the acid form of poly A has been investigated. The hydrocarbons were chosen to test a size criterion. Model building showed that some hydrocarbons could intercalate in the helix and be well protected from contact with the aqueous solvent. Experimentally it was...
Resource Type:: Dissertation
Full Text:: polycyclic, aromatic hydrocarbons to the acid form of poly A has been investigated. The hydrocarbons were

The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle

Creator:: Winzeler, Elizabeth A.
Abstract:: The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle were investigated by studying the time-resolved fluorescence anisotropy decays of ethidium bromide intercalated into the DNA of the core particle. As the torsional flexibility of DNA is affected by the presence of an intercalating...
Resource Type:: Masters Thesis
Full Text:: the molecular level in the past two decades have been astounding, there still remains a great deal of

Helical propensity of amino acids changes with solvent environment

Creator:: Krittanai, Chartchai
Resource Type:: Dissertation
Full Text:: structure of nucleic acids and polypeptides. The first two manuscripts deal with the correcting of CD

Base inclinations for synthetic nucleic acids in solution

Creator:: Jin, Xiaokui
Resource Type:: Dissertation
Full Text:: Base inclinations for synthetic nucleic acids in solution

Ribonucleotide Reductase Association with Mammalian Liver Mitochondria

Creator:: Chimploy, Korakod, Song, Shiwei, Wheeler, Linda J., and Mathews, Christopher K.
Abstract:: Deoxyribonucleoside triphosphate pools in mammalian mitochondria are highly asymmetric, and this asymmetry probably contributes toward the elevated mutation rate for the mitochondrial genome as compared with the nuclear genome. To understand this asymmetry, we must identify pathways for synthesis and accumulation of dNTPs within mitochondria. We have identified ribonucleotide reductase...
Resource Type:: Article
Full Text:: . Nucleic Acids Res. 39 , 1360–1371 3. Mathews, C. K., and Ji, J. (1992) DNA precursor asymmetries

Yeast POS5 NADH Kinase: Effects of a Mitochondrial Mutator Mutation on Mitochondrial Nucleotides

Creator:: Wheeler, Linda J. and Mathews, Christopher K.
Abstract:: Saccharomyces cerevisiae contains three NAD⁺/NADH kinases, one of which is localized in mitochondria and phosphorylates NADH in preference to NAD⁺. Strand et al (Euk. Cell 2:809 (2003)) reported that a yeast mutation in POS5, which encodes the mitochondrial NADH kinase, is a mutator, specific for mitochondrial genes. Because of the...
Resource Type:: Article
Full Text:: . Nucleic Acids Res. 38, e85. 17. Wheeler, L. J., Rajagopal, I., and Mathews, C. K. (2005) Stimulation of

Interactions of yeast dynein with dynein light chain and dynactin: General implications for intrinsically disordered duplex scaffolds in multi-protein assemblies

Creator:: Jie, Jing, Löhr, Frank, and Barbar, Elisar
Abstract:: Intrinsically disordered protein (IDP) duplexes composed of two IDP chains cross-linked by bivalent partner proteins form scaffolds for assembly of multiprotein complexes. The N-terminal domain of dynein intermediate chain (N-IC) is one such IDP that forms a bivalent scaffold with multiple dynein light chains including LC8, a hub protein that...
Resource Type:: Article
Full Text:: Workbench. Nucleic Acids Res. 41, W349–357 29. Peckham, M., and Knight, P. J. (2009) When a predicted

Characterization of UV-crosslinked protein-nucleic acid interfaces by Maldi MS and ESI MS/MS

Creator:: Gafken, Philip R.
Resource Type:: Dissertation
Full Text:: crosslinked to nucleic acids. Since these initial studies on peptide-nucleic acid complexes by FAB MS, major

Mitochondrial decay in the aging rat heart : changes in fatty acid-supported bioenergetics and macromolecular organization of the electron transport system

Creator:: Gómez Ramirez, Luis A.
Abstract:: Decline in cardiac pump function is a hallmark of aging where mitochondrial decay is an important underlying cause. Although certainly multifactorial in nature, both dysfunction of the machinery involved in the chemiosmotic process of energy transduction and lower capacity to maintain fatty acid-driven respiration are identified as intrinsic factors of...
Resource Type:: Dissertation
Full Text:: overall fatty acid -oxidation. Nevertheless, the exact molecular mechanism involved in the age-related

Analysis of secondary structures in nucleic acid binding proteins and nuclear magnetic resonance investigation of helix propagation and residual motions in proteins

Creator:: Hicks, Joshua M.
Abstract:: This thesis involves analyses of 3₂-helices, a-helices and of the change in residual entropy of a protein upon chemical modification. Part of the long-term goal of understanding the formation, function and stability of proteins requires that all conformations be accurately assigned and structurally understood. By a statistical analysis of 258...
Resource Type:: Dissertation
Full Text:: : Analysis of Secondary Structures in Nucleic Acid Binding Proteins and Nuclear Magnetic Resonance

A double-stranded secondary structure for hyaluronic acid in aqueous-organic solvent as revealed by vacuum ultraviolet circular dichroism

Creator:: Staskus, Paul
Abstract:: The association of hyaluronic acid (HA) into a double stranded structure has been investigated by circular dichroism (CD) spectroscopy into the vacuum ultraviolet region. The CD of HA changes dramatically, monitoring a cooperative transition as the dielectric constant of an aqueous solution is reduced by adding organic solvents. This structural...
Resource Type:: Dissertation
Full Text:: ) 0 H OH rn 7 glycosaminoglycans relative to proteins and nucleic acids has likely slowed

Age-related loss of Nrf2, a novel mechanism for the potential attenuation of xenobiotic detoxification capacity

Creator:: Smith, Eric Jonathan
Abstract:: Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2 or Nrf2) is the primary transcription factor in cellular defense against oxidative and xenobiotic stresses in higher eukaryotes. This basic leucine zipper transcription factor regulates over 200 antioxidant, detoxification, and lipid metabolizing genes by binding to the Antioxidant Response Element (ARE; a...
Resource Type:: Dissertation
Full Text:: ARE4. Having identified the Gclc-AREs in the rat we sought to describe the molecular consequence

Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques

Creator:: Codding, Sara Jone
Abstract:: The focus of this dissertation was to understand protein function and structure ona molecular level. To do this successfully, a variety of biochemical and biophysicaltechniques were employed. A wide variety of techniques were applied to study theproteins of interest in this dissertation to probe protein-protein interactions, proteinfunction, protein structure, and...
Resource Type:: Dissertation
Full Text:: examine many types of molecular interactions to analyze protein-protein, protein-nucleic ! 7! acid

Cloning, sequencing and aflatoxin B₁ metabolism by multiple rainbow trout CYP1A cDNAs expressed in yeast

Creator:: You, Lijing
Abstract:: Previous reports indicate the presence of multiple CYP1A sequences in rainbow trout, but their functional differences are unknown. This report describes the cloning and partial characterization of four trout CYP1A cDNAs, which are given the tentative designations CYP1A1v2, v3, v4, and v5. Comparison among these four and three previously reported...
Resource Type:: Masters Thesis
Full Text:: , 5 estrogens, corticosteroids, ecdysteroids, bile acids, vitamin D ), fatty acids and fatty acid

Fatty acids in the eggs and sac fry of steelhead trout (Salmo Gairdneri) : a developmental study

Creator:: Hayes, Lyle Wayne
Abstract:: Developing steelhead trout eggs and sac fry were examined for wet, dry and lipid weights and for fatty acid composition over the period from fertilization until the disappearance of the yolk sac, 69 days after fertilization. The yolk sac and embryo or fry were separated shortly before hatching, and the...
Resource Type:: Dissertation
Full Text:: 22:6w3. Since this acid is- not made de novo in the fish, and belongs to a family of acids found to

Purification of a ribonucleic acid-dependent ribonucleic acid polymerase from leukemic cells

Creator:: Watson, Kenneth Fredrick
Abstract:: There is good evidence that the infection with avian myeloblastosis virus BAI strain A causes myeloblasts to retain their primitive nature. These cells are able to multiply and produce virus at a relatively constant rate. From these observations, a major problem develops in the study of the virus-induced leukemia, that...
Resource Type:: Dissertation
Full Text:: INTRODUCTION 1 Virus-Infected Myeloblast Cells 1 BAI Strain A Myeloblastosis Virus The Nucleic Acid of the

Attempted cloning of T4 phage ribonucleotide reductase

Creator:: Booth, Joseph
Abstract:: Ribonucleotide reductase is an important enzyme in the control of DNA replication within the cell. Ribonucleotide reductase exerts its control through enzymatic reduction of nucleoside diphosphates. The bacteriophage T4 enzyme is an example of the class of iron-requiring reductases which also includes E. coli and mammalian ribonucleotide reductases. The two...
Resource Type:: Masters Thesis
Full Text:: are much longer, and more basic proteins than that of the rad B ORF and appear to be nucleic acid

Biosynthesis and enzymology of conjugated polyenoic fatty acid production in macrophytic marine algae

Creator:: Wise, Mitchell L.
Resource Type:: Dissertation
Full Text:: oxidized metabolites of arachidonate and eicosapentaenoic acid (EPA) as well as fatty acids of 16, 18

Novel icosanoids from the red marine alga Farlowia mollis

Creator:: Solem, Michele Lee
Abstract:: Farlowia mollis is a marine red alga that grows in the intertidal region along the coast of Oregon. We began studying the chemistry of this alga after a survey of Oregon seaweeds revealed possibly interesting chemistry and antimicrobial activity in its crude organic extract. Over the past three years the...
Resource Type:: Masters Thesis
Full Text:: the total fatty acid content).24 Seasonal variations in the contents of these fatty acids have been

In vivo detoxification of acetic acid in Saccharomyces cerevisiae

Creator:: Ding, Jun
Abstract:: The studies described in this thesis were motivated by ongoing efforts to develop lignocellulosic biomass as an efficient and practical source of renewable energy. Enormous problems complicate these efforts to reduce reliance on greenhouse gas-generating fossil fuels. Simply obtaining the fermentable sugars available in the cellulose and hemicellulose components of...
Resource Type:: Dissertation
Full Text:: -CoA metabolism in yeast to increase acetic acid tolerance or for the production of fatty acids and

Studies on the interaction of polylysine with deoxyribonucleic acid

Creator:: McKenzie, Raymond Leslie
Abstract:: The interaction of polylysine with DNA may be divided into two separate phenomena: the primary interaction involved in formation of a well-defined complex, and secondary interactions leading to aggregation of these complexes. Most studies on the interaction of polylysine with DNA have used techniques which measure properties of the aggregated...
Resource Type:: Dissertation
Full Text:: specificity. Though histones have not been found in procaryotic cells, protein-nucleic acid complexes have

Electron molecule interactions of amino acids and peptides

Creator:: Figard, Benjamin J.
Abstract:: Electron-biomolecule interactions are a biologically relevant field of study because there are several ion fragmentation techniques that have come to the forefront in mass spectrometric analysis that involve the interaction of charged peptides and free electrons. By studying the electron energies that cause fragmentation in neutral amino acids, amino acid...
Resource Type:: Dissertation
Full Text:: electron energies that cause fragmentation in neutral amino acids, amino acid derivatives, and peptides

Biochemical, molecular and physiological characterization of 1-butanol dehydrogenases of Pseudomonas butanovora in butane and 1-butanol metabolism

Creator:: Vangnai, Alisa S.
Abstract:: Butane-grown Pseudomonas butanovora oxidized butane by a soluble butane monooxygenase through the terminal pathway yielding 1 -butanol as the predominant product. Alcohol dehydrogenases (ADHs) involved in butane oxidation in P. butanovora were purified and characterized at the biochemical, genetic and physiological levels. Butane-grown P. butanovora expressed a type I soluble...
Resource Type:: Dissertation
Full Text:: , Molecular and Physiological Characterization of 1-Butanol Dehydrogenases of Pseudomonas butanovora in

Antioxidant mechanisms of ascorbate and (R)-α-lipoic acid in aging and transition metal ion-mediated oxidative stress

Creator:: Shu, Jung Hyuk
Abstract:: Oxidative stress is the major driving force behind the aging process and many age-related diseases. However, direct experimental evidence of whether antioxidants, such as ascorbate (AA) and lipoic acid (LA) can slow the progression of aging process and/or reduce risks of developing degenerative disease is largely absent. This suggests a...
Resource Type:: Dissertation
Full Text:: Mechanisms of Ascorbate and (R)-Alpha-Lipoic Acid in Aging and Transition Metal Ion-Mediated Oxidative Stress

A partial characterization of the deoxyribonucleic acid found in the thoracic muscles of the tobacco hornworm moth (Manducta sexta)

Creator:: Hinds, Thomas Richard, 1944-
Abstract:: The DNAs (deoxyribonucleic acids) of the thoracic tissue of the tobacco hornworm, Manducta sexta, were studied by physical and chemical means. The adult moth was considered to be fully formed two days after emergence from the pupal case, as indicated by its ability to fly and the integrity of the...
Resource Type:: Dissertation
Full Text:: . Aliquots were removed for the protein and nucleic acid assays. The nucleic acids were precipitated with an

Investigations of the isoprenoid nucleosides of pea root transfer ribonucleic acid

Creator:: Babcock, Donner Franklin, 1942-
Resource Type:: Dissertation
Full Text:: presented evidence for the existence of cytoplasmic nucleic acid and protein containing par- ticles

The effect of selenium deficiency on the fatty acid metabolism of rats and lambs

Creator:: Fischer, William Carl
Abstract:: Selenium deficiency in rats results in increased carbon metabolism, approaching twice the rate of selenium supplemented animals (normal). This is also reflected in a 50 percent increase in lysosomal enzyme activity in tissues of selenium deficient animals. Isolation of rat liver lysosomes shows a 17 percent increase in membrane thickness...
Resource Type:: Dissertation
Full Text:: reported that vitamin E not only controls the synthesis of ribo- nucleic acid but subsequent protein

Biosyntheis of Blasticidin S : pathway and enzymes for the nucleoside formation and blastidic acid assembly

Creator:: Guo, Jincan
Resource Type:: Dissertation
Full Text:: had been shown that BS did not inhibit the incorporation of 32P into nucleic acids at concentrations

Dependence of secondary structure of biopolymers on environment : a circular dichroism study of equivocal amino acid sequences in proteins and of left-handed DNA

Creator:: Zhong, Lingxiu
Resource Type:: Dissertation
Full Text:: environment : a circular dichroism study of equivocal amino acid sequences in proteins and of left-handed DNA

The effect of dietary compounds on human cathelicidin antimicrobial peptide gene expression mediated through farnesoid X receptor and its potential role in gastrointestinal health

Creator:: Campbell, Yan
Abstract:: The human cathelicidin antimicrobial peptide (CAMP) is a broad spectrum microbicidal agent and modulator of both the innate and adaptive immune system. It is induced by 1,25-dihydroxyvitamin D (1,25(OH)₂D₃) through activation of the vitamin D receptor (VDR) and primary bile salts through activation of the xenobiotic nuclear receptor farnesoid X...
Resource Type:: Dissertation
Full Text:: two major primary bile acids are cholic acid (CA) and chenodeoxycholic acid (CDCA) (86). CDCA

Uracil-DNA glycosylase inhibitor protein : role of carboxylic acid residues and use for measuring the fidelity of uracil-excision DNA repair synthesis in human cell extracts

Creator:: Sanderson, Russell J.
Resource Type:: Dissertation
Full Text:: acid sequence of bacteriophage PBS2 uracil-DNA glycosylase inhibitor protein 161 22. Molecular mass

Exogenous gene expression from heterologous promoters in fish cell cultures

Creator:: Sharps, Angela
Abstract:: Cell culture systems have provided many insights into eukaryotic gene expression and other biochemical mechanisms. Since the cell represents the smallest living unit of any organism it provides a desirable in vitro system, allowing biochemical studies without the complex physiology of an entire animal. However, processes involving intracellular mechanisms, such...
Resource Type:: Masters Thesis
Full Text:: of heterologous genes under the influence of promoter and other regulatory nucleic acid aequences

Analyses of noncanonical DNA structures

Creator:: Khuu, Patricia A.
Abstract:: The structural polymorphic nature of DNA has been long been recognized since the model of the right-handed B-DNA duplex proposed by James Watson and Francis Crick was accepted. Its malleability is thought to be critical in protein recognition and manipulation. In particular, it can form Holliday junctions, four-way DNA structural...
Resource Type:: Dissertation
Full Text:: recombination mediated events in which four nucleic acid strands complex to give rise to double-helical arms

Biological activity of avian myeloblastosis virus RNA in cell-free systems for protein synthesis

Creator:: Olson, Kenneth Charles
Abstract:: Avian myeloblastosis virus RNA was fractionated into a high molecular weight RNA and a low molecular weight RNA fraction by sucrose density centrifugation. The larger RNA component had a sedimentation constant of 65S and the low molecular weight RNA about 4S. The low molecular RNA has two biological properties of...
Resource Type:: Masters Thesis
Full Text:: these amino acids act in protein synthesis and are not involved in the synthesis of nucleic acid build

Base inclinations in natural and synthetic DNAs

Creator:: Chou, Ping-Jung
Abstract:: A sophisticated computer program is developed to analyze flow linear dichroism data on nucleic acids for individual base inclinations. Measured absorption and linear dichroism data for synthetic AT and GC polymers and natural DNAs are analyzed. The reliability of the program is tested on data for the synthetic polymers, and...
Resource Type:: Dissertation
Full Text:: dichroism data on nucleic acids for individual base inclinations. Measured absorption and linear dichroism

Macromolecular halogen bonds

Creator:: Voth, Andrea Regier
Abstract:: The halogen bond is a non-covalent, stabilizing interaction analogous to a hydrogen bond in which an anisotropically polarized halogen atom interacts electrostatically with a Lewis base. Until very recently, the ability of halogens to form these stabilizing interactions in biological macromolecules was all but unknown, but examples of halogen bonding...
Resource Type:: Dissertation
Full Text:: observed in nucleic acids as well as protein complexes with hormones, drugs and inhibitors. The lack of

Crystal structures of DNA four-way junctions

Creator:: Eichman, Brandt F.
Abstract:: DNA four-way junctions (also known as Holliday junctions) are the primary structural intermediate during recombination, an important process responsible for biological evolution and maintenance of genomes. These junctions arise from the assembly of four nucleic acid strands to produce double-helical regions extending from a central point. Although much progress has...
Resource Type:: Dissertation
Full Text:: evolution and maintenance of genomes. These junctions arise from the assembly of four nucleic acid strands

SLC23A1, the gene encoding sodium-dependent vitamin C transport protein 1 (SVCT1) : regulation of transcription and its functional consequences

Creator:: Michels, Alexander Johannes
Abstract:: Vitamin C is an essential component of the human diet. Uptake of vitamin C in the cell is regulated by the function of sodium-dependent vitamin C transporters (SVCTs). SVCT1 (encoded by the SLC23A1 gene) is expressed in the colon, liver and kidney, where it plays a vital role in determining...
Resource Type:: Dissertation
Full Text:: - Lipoic Acid (LA), significantly elevated reporter gene expression over controls. LA did not elicit its

Chromatin structure : possible regulatory role in gene expression

Creator:: Herman, Timothy Merle
Abstract:: Chromatin DNA sequences intimately associated with the "core" of the basic, repeating chromatin subunit are shown to be relatively resistant to digestion by micrococcal nuclease. Chromatin DNA sequences lying between adjacent chromatin subunits, as well as DNA sequences in regions of chromatin which are free of chromatin subunits, are subject...
Resource Type:: Dissertation
Full Text:: located. Nuclease digestion of chromatin is used, together with nucleic acid hybridization, to determine

The binding of ethidium bromide to chromatin : model for carcinogen interactions

Creator:: McMurray, Cynthia T.
Abstract:: Five forms of chromatin, which represent the in vivo folded forms of nucleic acid, were isolated and used as the binding substrate for model intercalating compound, ethidium bromide. For all forms of chromatin, the affinity, location and structural effects of binding were examined. On the level of the nucleosome, the...
Resource Type:: Dissertation
Full Text:: Five forms of chromatin, which represent the in vivo folded of nucleic acid, were isolated and used as

Alternatives to the DNA precursor-synthesizing enzyme complex hypothesis

Creator:: Weitz, Stephen L.
Abstract:: Investigators have been studying an aggregate containing enzymes of deoxyribonucleoside triphosphate biosynthesis in T4-phage infected bacteria. They suggest that it behaves as an organized complex, efficiently channeling DNA precursors to the replication apparatus. Previous work has suggested that about 10 enzyme activities remain associated through several fractionation steps. This multi-enzyme...
Resource Type:: Dissertation
Full Text:: enzyme binds to ribosomal subunits due to an affinity for single-stranded nucleic acids under conditions

Deoxyribose-phosphate aldolase and the biosyntheiss of DNA in the chick embryo

Creator:: Anderson, Martin Michael
Abstract:: The activity of deoxyribose-phosphate aldolase was determined in chick embryo brains, hearts and livers during the course of development. Assays were made in the direction of synthesis of deoxyribose-5-phosphate. Low levels of activity in the brain and heart remained constant throughout the course of development. Activity in the liver expressed...
Resource Type:: Dissertation
Full Text:: and radioactivity in liver nucleic acid extracts. 51 7 DNA and RNA contents and specific activities

Calpain 2 proteolysis regulates glioblastoma cell invasion

Creator:: Lal, Sangeet Kumar
Abstract:: Glioblastoma is the most malignant primary brain tumor with the average patients surviving only one year after diagnosis, even with aggressive therapy. The formation of numerous micro-tumors dispersed into the brain due to rapid invasion of tumor cells, presents the primary challenge to the surgical removal of tumors and limits...
Resource Type:: Dissertation
Full Text:: diagnosis, even with aggressive therapy. The formation of numerous micro-tumors dispersed into the brain

Regulation of phosphofructokinase by reversible inactivation

Creator:: Zhao, Zhizhuang
Abstract:: The interaction of skeletal muscle phosphofructokinase with a variety of acidic proteins including calmodulin and troponin C and with nucleic acids (RNA and DNA) is manifest in a reversible, time-dependent loss of catalytic activity. The inactivation is affected by a number of factors. Substrates (fructose-6-phosphate and ATP), positive effectors (fructose-2,6-bisphosphate,...
Resource Type:: Dissertation
Full Text:: phosphofructokinase with a variety of acidic proteins including calmodulin and troponin C and with nucleic acids

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Creator:: Sargent, Roy Geoffrey
Abstract:: Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic for DNA synthesis in both intact cells and cell-free replication systems. Almost certainly, such mutagenesis involves competition between correctly and incorrectly base-paired precursors at replication sites. However, there are certain differences between the intact cell and cell-free systems that do not always allow...
Resource Type:: Dissertation
Full Text:: mutagenesis, which was confirmed by nucleic acid sequencing of amber+ phage revertants. To some degree, the

Peroxiredoxins in Parasites

Creator:: Gretes, Michael C., Poole, Leslie B., and Karplus, P. Andrew
Abstract:: Significance: Parasite survival and virulence relies on effective defenses against reactive oxygen and nitrogen species produced by the host immune system. Peroxiredoxins (Prxs) are ubiquitous enzymes now thought to be central to such defenses and, as such, have potential value as drug targets and vaccine antigens. Recent Advances: Plasmodial and...
Resource Type:: Article
Full Text:: efficiently detoxify peroxyni- trite/peroxynitrous acid (ONOO -/ONOOH; pKa = 6.8), a powerful oxidant and

Dietary organosulfur and organoselenium compounds as HDAC inhibitors

Creator:: Nian, Hui
Abstract:: Histone deacetylase (HDAC) inhibitors have the potential to de-repress epigenetically silenced genes in cancer cells, leading to cell cycle arrest and apoptosis. Dietary HDAC inhibitors derived from natural phytochemicals are promising anticancer agents. In this thesis, metabolites from natural organosulfur and organoselenium compounds, i.e. allyl mercaptan (AM), β-methylselenopyruvate (MSP) and...
Resource Type:: Dissertation
Full Text:: newly discovered α‐keto acid metabolites of Se‐methylselenocysteine (MSC) and

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