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Filtering by: Creator Potter, Nathan D. Remove constraint Creator: Potter, Nathan D. Academic Affiliation Biochemistry and Biophysics Remove constraint Academic Affiliation: Biochemistry and Biophysics

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  • Metabolic pathway of the nitrosoureas catalyzed by cytochrome P-450 and NADPH cytochrome P-450 reductase

    Creator:
    Potter, David Willard
    Abstract:
    Three highly purified forms of cytochrome P-450 (P-450a, P-450b, P-450c) from Aroclor 1254-induced rat hepatic microsomes catalyzed the monooxygenation of 1-(2- chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea (MeCCNU) and 1-(2-chloroethyl)-3-(cyclohexyl)-1-nitrosourea (CCNU) in the presence of purified NADPH cytochrome P-450 reductase, NADPH and oxygen. Cytochrome P -450- dependent monooxygenation of CCNU gave six alicyclic hydroxylation products...
    Resource Type:
    Dissertation
    Full Text:
    , 1982 Typed by Lan D. Do for David Willard Potter ACKNOWLEDGMENTS I thank my mentor, Professor

  • Viral Targeting of Host Hubs: Interactions between the Rabies Virus and LC8

    Creator:
    Jespersen, Nathan E.
    Abstract:
    As obligate intracellular parasites, viruses rely on host machinery for their own reproduction. Viruses are therefore required to interact with a wide variety of host proteins, despite limitations in viral genome size. The most parsimonious method of success is to hijack central and essential proteins, known as hub proteins. LC8...
    Resource Type:
    Dissertation
    Full Text:
    AN ABSTRACT OF THE DISSERTATION OF Nathan E

  • Studies on the growth inhibition and differentiation of serum-free mouse embryo (SFME) cells

    Creator:
    Varga Weisz, Patrick D.
    Abstract:
    Serum-free mouse embryo (SFME) cells are derived in medium in which serum is replaced with growth factors and other supplements. They display unusual properties. They do not lose proliferative potential or show gross chromosomal aberration upon extended culture, they depend on epidermal growth factor (EGF) for survival, and are reversibly...
    Resource Type:
    Dissertation
    Full Text:
    serum-free mouse embryo (SFME) cells AN ABSTRACT OF THE THESIS OF Patrick D. Varga Weisz for the

  • Mercury and cadmium binding proteins in marine mammal tissues

    Creator:
    von der Trenck, Karl Theodor
    Abstract:
    The subcellular fractions of liver and kidney from three bearded seals (Erignathus barbatus nauticus) and three walrus (Odobenus rosmarus divergens) were analyzed for cadmium and mercury content, and the results were compared with the results of subcellular fractionation of the same organs from two sea lions (Zalophus callfornianus californianus) obtained...
    Resource Type:
    Masters Thesis
    Full Text:
    : Dr. D. .K. The subcellular fractions of liver and kidney from three bearded seals (Erignathus

  • Specific enzymic interactions relating to the mechanism of action on certain 2-chloroethyl nitrosoureas

    Creator:
    Babson, John Robert
    Abstract:
    Certain 2- chioroethyl nitrosoureas have been demonstrated to be highly specific inactivators of chymotrypsin in vitro and glutathione reductase, both in vivo and in isolated rat hepatocytes. The inactivation of glutathione reductase in isolated hepatocytes resulted in an increased sensitivity toward an adriamycin induced oxidative challenge. Prolonged incubation of 1-(2-chloroethyl)-3-([1-¹⁴...
    Resource Type:
    Dissertation

  • Pyruvate kinase isozymes in beef and chicken

    Creator:
    Strandholm, John Jeffrey, 1945-
    Resource Type:
    Dissertation
    Full Text:
    for privacyAbstract approved: Robert D. Dy on Redacted for privacy Janet M. Cardenas At least three

  • Studies on the inhibition of bovine plasma amine oxidase by hydrazines

    Creator:
    Hucko-Haas, Jacqueline Elizabeth
    Abstract:
    Several aspects of the potent inhibition of bovine plasma amine oxidase (PAO) by hydrazines were investigated by kinetic and preparative means. The inhibition was classified as pseudo-irreversible by the method of Ackerman and Potter (Proc. Soc. Exptl. Biol. Med. 72, 1 (1949), and was found to exhibit Zone B kinetic...
    Resource Type:
    Dissertation
    Full Text:
    -Haas for the Ph. D. (Name) (Degree) in Biochemistry presented on it02))4,) 9(. (Major) (Date) Title

  • Automethylation : a response to enzyme aging

    Creator:
    Lindquist, Jonathan A.
    Abstract:
    This is the first study to explore the ability of an enzyme to recognize and repair spontaneous age-dependent damage to its own sequence. Protein (D-aspartyl/L-isoaspartyl) carboxyl methyltransferase (PCM) is known to repair damage that arises from a spontaneous isomerization of aspartyl and asparaginyl residues in other proteins during aging. As...
    Resource Type:
    Dissertation
    Full Text:
    and repair spontaneous age-dependent damage to its own sequence. Protein (D-aspartyl/ L­

  • Characterization of neuronal nitric-oxide synthase reductase activity

    Creator:
    Wolthers, Kirsten R.
    Abstract:
    During catalysis the flavoprotein domain of neuronal nitric-oxide synthase (nNOS) shuttles NADPH-derived reducing equivalents from FAD to FMN and then to the P450-heme enabling heme-based oxygen activation and subsequent NO-synthesis. The binding of Ca²⁺-activated calmodulin (Ca²⁺-CaM) to nNOS alleviates inhibition of flavin mediated electron transfer within the diflavin domain, which...
    Resource Type:
    Dissertation
    Full Text:
    found to abstract the pro-R (A-side) hydrogen from NADPH. Primary deuterium isotope effects (NADP(D

  • Structural characterization of the cardiac muscarinic acetylcholine receptor

    Creator:
    Rosenbaum, Larry C.
    Abstract:
    The physical properties of the purified cardiac muscarinic acetylcholine receptor have been examined by D₂O/H₂O sucrose gradient sedimentation and sephacryl S-300 gel filtration in Triton X-405 and dodecyl-β-maltoside. From the sedimentation experiments, the partial specific volume of the receptor-Triton X-405 complex were 0.813 cm³/g and 5.30 S, respectively and the...
    Resource Type:
    Masters Thesis
    Full Text:
    Biophysics Redacted for Privacy Dean of Graduat School d Date thesis is presented May 4, 1987 Typed by