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Filtering by: Creator Williamson, Kenneth J. Academic Affiliation Botany and Plant Pathology Creator Arp, Daniel J.

Kinetic Mechanism of the Hydrogen-Oxidizing Hydrogenase from Soybean Nodule Bacteroids

Creator:

Arp, Daniel J. and Burris, Robert H.

Abstract:

The kinetic mechanism of the unidirectional H2-oxidizing hydrogenase from soybean nodule bacteroids has been investigated with highly purified enzyme. Measurements of the K, for H2 vary from 0.97 to 2.6 pM, and the K,,, for methylene blue varies from 6 to 17 pM. With H2 and methylene blue as substrates,...

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Hydrogen-Oxidizing Hydrogenase from Soybean Nodule Bacteroids? Daniel J. Arp and Robert H. Burris
Kinetic and Spectroscopic Analysis of the Inactivating Effects of Nitric Oxide on the Individual Components of Azotobacter vinelandii Nitrogenase

Creator:

Hyman, Michael R., Seefeldt, Lance C., Morgan, Vance, Arp, Daniel J., and Mortenson, Leonard E.

Abstract:

The effects of nitric oxide (NO) on the individual components of Azotobacter vinelandii nitrogenase have been examined by kinetic and spectroscopic methods. Incubation of the Fe protein (Av2) for 1 h with stoichiometries of 4- and 8-fold molar excesses of NO to Av2 dimer resulted in a complete loss of...

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vinelandii Nitrogenasef Michael R. Hyman,* Lance C. Seefeldt,$ T. Vance Morgan,$ Daniel J. Arp,* and