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Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline

Creator:: Gu, Weifeng, Shirayama, Masaki, Conte Jr., Darryl, Vasale, Jessica, Batista, Pedro J., Claycomb, Julie M., Moresco, James J., Youngman, Elaine, Keys, Jennifer, Stoltz, Matthew J., Chen, Chun-Cheih G., Chaves, Daniel A., Duan, Shenghua E., Kasschau, Krisitin D., Fahlgren, Noah, Yates III, John R., Mitani, Shohei, Carrington, James C., and Mello, Craig C.
Abstract:: Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the dicer-related helicase, drh-3, that abrogate both RNA interference and...
Resource Type:: Article
Full Text:: results in the 2′-O-methylation of the 3′ residue (Klattenhoff and Theurkauf, 2008). In plants, fungi and

Computational and analytical framework for small RNA profiling by high-throughput sequencing

Creator:: Fahlgren, Noah, Sullivan, Christopher M., Kasschau, Krisitin D., Chapman, Elisabeth J., Cumbie, Jason S., Montgomery, Taiowa A., Gilbert, Sunny D., Dasenko, Mark, Backman, Tyler W. H., Givan, Scott A., and Carrington, James C.
Abstract:: The advent of high-throughput sequencing (HTS) methods has enabled direct approaches to quantitatively profile small RNA populations. However, these methods have been limited by several factors, including representational artifacts and lack of established statistical methods of analysis. Furthermore, massive HTS data sets present new problems related to data processing and...
Resource Type:: Article
Full Text:: products with a 59 monophosphate and 39 hydroxyl. Endogenous classes of 59 monophosphate-containing short

High-Throughput Sequencing of Arabidopsis microRNAs : Evidence for Frequent Birth and Death of MIRNA Genes

Creator:: Fahlgren, Noah, Howell, Miya D., Kasschau, Kristin D., Sullivan, Christopher M., Cumbie, Jason S., Givan, Scott A., Law, Theresa F., Grant, Sarah R., Dangl, Jeffrey L., and Carrington, James C.
Abstract:: In plants, microRNAs (miRNAs) comprise one of two classes of small RNAs that function primarily as negative regulators at the posttranscriptional level. Several MIRNA genes in the plant kingdom are ancient, with conservation extending between angiosperms and the mosses, whereas many others are more recently evolved. Here, we use deep...
Resource Type:: Article
Full Text:: w o r re ce n tl y id e n ti fi e d m iR N A s

Genome-Wide Profiling and Analysis of Arabidopsis siRNAs

Creator:: Kasschau, Kristin D., Fahlgren, Noah, Chapman, Elisabeth J., Sullivan, Christopher M., Cumbie, Jason S., Givan, Scott A., and Carrington, James C.
Abstract:: Eukaryotes contain a diversified set of small RNA-guided pathways that control genes, repeated sequences, and viruses at the transcriptional and posttranscriptional levels. Genome-wide profiles and analyses of small RNAs, particularly the large class of 24-nucleotide (nt) short interfering RNAs (siRNAs), were done for wild-type Arabidopsis thaliana and silencing pathway mutants...
Resource Type:: Article
Full Text:: sequenced using high-throughput methods (Figure 1A). Amplicons were prepared by 59 and 39 adaptor ligation

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Creator:: Schadd, Mary C., Haldeman-Chahill, Ruth, Cronin, Stephen, and Carrington, James C.
Abstract:: A mutational analysis was conducted to investigate the functions of the tobacco etch potyvirus VPgproteinase (NIa) protein in vivo. The NIa N-terminal domain contains the VPg attachment site, whereas the C-terminal domain contains a picornavirus 3C-like proteinase. Cleavage at an internal site separating the two domains occurs in a subset...
Resource Type:: Article
Full Text:: ) polyclonal anti- bodies and anti-NIa monoclonal antibodies were described previously (33, 39). Transgenic

Host-Specific Involvement of the HC Protein in the Long-Distance Movement of Potyviruses

Creator:: Sáenz, Pilar, Salvador, Beatriz, Simón-Mateo, Carmen, Kasschau, Kristin D., Carrington, James C., and García, Juan Antonio
Abstract:: Plum pox virus (PPV) is a member of the Potyvirus genus that, in nature, infects trees of the Prunus genus. Although PPV infects systemically several species of the Nicotiana genus, such as N. clevelandii and N. benthamiana, and replicates in the inoculated leaves of N. tabacum, it is unable to...
Resource Type:: Article
Full Text:: transmission of potyviruses. Proc. Natl. Acad. Sci. USA 86:8402–8406. 7. Brigneti, G., O. Voinnet, W. X. Li, L

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Creator:: Carrington, James C., Freed, Deon D., and Sanders, Tatia C.
Abstract:: The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage event catalyzed by HC-Pro...
Resource Type:: Article
Full Text:: toward understand- ing the mechanisms and requirements involved in TEV ...o= ss. ".. m-'0 *-...--...s

Phytophthora Have Distinct Endogenous Small RNA Populations That Include Short Interfering and microRNAs

Creator:: Fahlgren, Noah, Bollmann, Stephanie R., Kasschau, Kristin D., Cuperus, Josh T., Press, Caroline M., Sullivan, Christopher M., Chapman, Elisabeth J., Hoyer, J. Steen, Gilbert, Kerrigan B., Grunwald, Niklaus J., and Carrington, James C.
Abstract:: In eukaryotes, RNA silencing pathways utilize 20-30-nucleotide small RNAs to regulate gene expression, specify and maintain chromatin structure, and repress viruses and mobile genetic elements. RNA silencing was likely present in the common ancestor of modern eukaryotes, but most research has focused on plant and animal RNA silencing systems. Phytophthora...
Resource Type:: Article
Full Text:: by northern blot assay (Figure 6D). In plants, miRNA are protected from 39–.59 degradation by 29-O

Genetic and Functional Diversification of Small RNA Pathways in Plants

Creator:: Xie, Zhixin, Johansen, Lisa K., Gustafan, Adam M., Kasschau, Kristin D., Lellis, Andrew D., Zilberman, Daniel, Jacobsen, Steven E., and Carrington, James C.
Abstract:: Multicellular eukaryotes produce small RNA molecules (approximately 21–24 nucleotides) of two general types, microRNA (miRNA) and short interfering RNA (siRNA). They collectively function as sequence-specific guides to silence or regulate genes, transposons, and viruses and to modify chromatin and genome structure. Formation or activity of small RNAs requires factors belonging...
Resource Type:: Article
Full Text:: DNA oligonucleotide AS-159 (59- TAGAGCTCCCTTCAATCCAAA-39). siRNA02 and siRNA1003 were detected using

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
Resource Type:: Article
Full Text:: the virus by aphids, efficient RNA replication, and systemic movement through plants (6, 7, 18, 22, 39

ScholarsArchive@OSU

Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline

Computational and analytical framework for small RNA profiling by high-throughput sequencing

High-Throughput Sequencing of Arabidopsis microRNAs : Evidence for Frequent Birth and Death of MIRNA Genes

Genome-Wide Profiling and Analysis of Arabidopsis siRNAs

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Host-Specific Involvement of the HC Protein in the Long-Distance Movement of Potyviruses

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Phytophthora Have Distinct Endogenous Small RNA Populations That Include Short Interfering and microRNAs

Genetic and Functional Diversification of Small RNA Pathways in Plants

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

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