Index Catalog // ScholarsArchive@OSU

Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline

Creator:: Gu, Weifeng, Shirayama, Masaki, Conte Jr., Darryl, Vasale, Jessica, Batista, Pedro J., Claycomb, Julie M., Moresco, James J., Youngman, Elaine, Keys, Jennifer, Stoltz, Matthew J., Chen, Chun-Cheih G., Chaves, Daniel A., Duan, Shenghua E., Kasschau, Krisitin D., Fahlgren, Noah, Yates III, John R., Mitani, Shohei, Carrington, James C., and Mello, Craig C.
Abstract:: Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the dicer-related helicase, drh-3, that abrogate both RNA interference and...
Resource Type:: Article
Full Text:: surveillance in the C. elegans germline Weifeng Gu1,8, Masaki Shirayama1,8, Darryl Conte Jr.1,8,*, Jessica

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Creator:: Li, Xiao Hua and Carrington, James C.
Abstract:: A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)- expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A β-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA amplification. Two mutants (termed VNN...
Resource Type:: Article
Full Text:: . A Virus - TEV-GU Plant - Vector B Vector C D VI-GUS VNN Nlb+ Nlb+ FIG. 5. Infection of vector

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: Tobacco etch virus (TEV) encodes three proteinases that catalyze processing of the genome-encoded polyprotein. The P1 proteinase originates from the N terminus of the polyprotein and catalyzes proteolysis between itself and the helper component proteinase (HC-Pro). Mutations resulting in substitution of a single amino acid, small insertions, or deletions were...
Resource Type:: Article
Full Text:: with the letter codes A1, A2, A, B, C, D, E, and F, respectively. The S256A mutation, resulting in

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
Resource Type:: Article
Full Text:: . Virology 178:573–578. 53. Vance, V. B., D. Moore, T. H. Turpen, A. Bracker, and V. C. Hollowell. 1992. The

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Creator:: Schadd, Mary C., Haldeman-Chahill, Ruth, Cronin, Stephen, and Carrington, James C.
Abstract:: A mutational analysis was conducted to investigate the functions of the tobacco etch potyvirus VPgproteinase (NIa) protein in vivo. The NIa N-terminal domain contains the VPg attachment site, whereas the C-terminal domain contains a picornavirus 3C-like proteinase. Cleavage at an internal site separating the two domains occurs in a subset...
Resource Type:: Article
Full Text:: (X -G lu c) ,w hi ch fo rm s a bl ue re ac tio n pr od uc t in th e pr es en ce of G U S. B ar s eq

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Creator:: Carrington, James C., Freed, Deon D., and Sanders, Tatia C.
Abstract:: The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage event catalyzed by HC-Pro...
Resource Type:: Article
Full Text:: the 47-kDa precursor and 37-kDa proteolytic product are shown between panels A and B. (C) Quantitation

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

Creator:: Carbonell, Alberto, Fahlgren, Noah, Garcia-Ruiz, Hernan, Gilbert, Kerrigan B., Montgomery, Taiowa A., Nguyen, Tammy, Cuperus, Josh T., and Carrington, James C.
Abstract:: In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicer-independent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were...
Resource Type:: Article
Full Text:: Kerrigan B. Gilbert,a Taiowa A. Montgomery,b,1 Tammy Nguyen,b Josh T. Cuperus,b,2 and James C. Carringtona

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Creator:: Carrington, James C. and Freed, Deon D.
Abstract:: The RNA genome of tobacco etch virus (TEV), a plant potyvirus, functions as an mRNA for synthesis of a 346-kilodalton polyprotein that undergoes extensive proteolytic processing. The RNA lacks a normal 5' cap structure at its terminus, which suggests that the mechanism of translational initiation differs from that of a...
Resource Type:: Article
Full Text:: . C. Milburn, I. Edery, N. Sonenberg, and J. W. B. Hershey. 1982. Inhibition of HeLa cell protein

Phytophthora Have Distinct Endogenous Small RNA Populations That Include Short Interfering and microRNAs

Creator:: Fahlgren, Noah, Bollmann, Stephanie R., Kasschau, Kristin D., Cuperus, Josh T., Press, Caroline M., Sullivan, Christopher M., Chapman, Elisabeth J., Hoyer, J. Steen, Gilbert, Kerrigan B., Grunwald, Niklaus J., and Carrington, James C.
Abstract:: In eukaryotes, RNA silencing pathways utilize 20-30-nucleotide small RNAs to regulate gene expression, specify and maintain chromatin structure, and repress viruses and mobile genetic elements. RNA silencing was likely present in the common ancestor of modern eukaryotes, but most research has focused on plant and animal RNA silencing systems. Phytophthora...
Resource Type:: Article
Full Text:: , Elisabeth J. Chapman2¤b, J. Steen Hoyer1, Kerrigan B. Gilbert1, Niklaus J. Grünwald3*, James C

Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

Creator:: Garcia-Ruiz, Hernan, Carbonell, Alberto, Hoyer, J. Steen, Fahlgren, Noah, Gilbert, Kerrigan B., Takeda, Atsushi, Giampetruzzi, Annalisa, Garcia Ruiz, Mayra T., McGinn, Michaela G., Lowery, Nicholas, Martinez Baladejo, Maria T., and Carrington, James C.
Abstract:: In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
Resource Type:: Article

ScholarsArchive@OSU

Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Phytophthora Have Distinct Endogenous Small RNA Populations That Include Short Interfering and microRNAs

Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

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