Index Catalog // ScholarsArchive@OSU

Creator:: Zhalnina, Kateryna, de Quadros, Patricia D., Gano, Kelsey A., Davis-Richardson, Austin, Fagen, Jennie R., Brown, Christopher T., Giongo, Adriana, Drew, Jennifer C., Sayavedra-Soto, Luis A., Arp, Daniel J., Camargo, Flavio A. O., Daroub, Samira H., Clark, Ian M., McGrath, Steve P., Hirsch, Penny R., and Triplett, Eric W.
Abstract:: Agricultural land management, such as fertilization, liming, and tillage affects soil properties, including pH, organic matter content, nitrification rates, and the microbial community. Three different study sites were used to identify microorganisms that correlate with agricultural land use and to determine which factors regulate the relative abundance of the microbial...
Resource Type:: Article

Creator:: Arp, Daniel J. and Burris, Robert H.
Abstract:: The kinetic mechanism of the unidirectional H2-oxidizing hydrogenase from soybean nodule bacteroids has been investigated with highly purified enzyme. Measurements of the K, for H2 vary from 0.97 to 2.6 pM, and the K,,, for methylene blue varies from 6 to 17 pM. With H2 and methylene blue as substrates,...
Resource Type:: Article

Creator:: Hyman, Michael R., Ensign, Scott A., Arp, Daniel J., and Ludden, Paul W.
Abstract:: Carbonyl sulfide (COS) has been investigated as a rapid-equilibrium inhibitor of CO oxidation by the CO dehydrogenase purified from Rhodospirillum rubrum. The kinetic evidence suggests that the inhibition by COS is largely competitive versus CO (Ki = 2.3 pM) and uncompetitive versus methylviologen as electron acceptor (Ki = 15.8 pM)....
Resource Type:: Article

Creator:: Hyman, Michael R., Seefeldt, Lance C., Morgan, Vance, Arp, Daniel J., and Mortenson, Leonard E.
Abstract:: The effects of nitric oxide (NO) on the individual components of Azotobacter vinelandii nitrogenase have been examined by kinetic and spectroscopic methods. Incubation of the Fe protein (Av2) for 1 h with stoichiometries of 4- and 8-fold molar excesses of NO to Av2 dimer resulted in a complete loss of...
Resource Type:: Article

Creator:: Sun, Jin-Hua, Hyman, Michael R., and Arp, Daniel J.
Abstract:: Acetylene is a slow-binding inhibitor of the Ni- and Fe-containing dimeric hydrogenase isolated from Azotobacter vinelandii. Acetylene was released from hydrogenase during the recovery from inhibition. This indicates that no transformation of acetylene to another compound occurred as a result of the interaction with hydrogenase. However, the release of C2H2...
Resource Type:: Article

Creator:: Starkenburg, Shawn R., Arp, Daniel J., and Bottomley, Peter J.
Abstract:: Nitrobacter hamburgensis X14 is a facultative lithoautotroph that conserves energy from the oxidation of nitrite (NO 2 ) and fixes carbon dioxide (CO2) as its sole source of carbon. The availability of the N. hamburgensis X14 genome sequence initiated a re-examination of its mixotrophic and organotrophic potential, as genes encoding...
Resource Type:: Article

Creator:: Dubbels, Bradley L., Sayavedra-Soto, Luis A., Bottomley, Peter J., and Arp, Daniel J.
Abstract:: The placement of ‘Pseudomonas butanovora’ in the genus Thauera was proposed previously, based on 16S rRNA gene sequence analysis, upon further studies of taxonomical characteristics. In this study, physiological characteristics and DNA–DNA reassociation data are presented and the transfer of ‘P. butanovora’ to the genus Thauera is proposed. The original...
Resource Type:: Article

Creator:: Cooley, Richard B., Dubbels, Bradley L., Sayavedra-Soto, Luis A., Bottomley, Peter J., and Arp, Daniel J.
Abstract:: Soluble butane monooxygenase (sBMO), a three-component di-iron monooxygenase complex expressed by the C2–C9 alkane-utilizing bacterium Thauera butanivorans, was kinetically characterized by measuring substrate specificities for C1–C5 alkanes and product inhibition profiles. sBMO has high sequence homology with soluble methane monooxygenase (sMMO) and shares a similar substrate range, including gaseous and...
Resource Type:: Article

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