Index Catalog // ScholarsArchive@OSU

Genome-Wide Profiling and Analysis of Arabidopsis siRNAs

Creator:: Kasschau, Kristin D., Fahlgren, Noah, Chapman, Elisabeth J., Sullivan, Christopher M., Cumbie, Jason S., Givan, Scott A., and Carrington, James C.
Abstract:: Eukaryotes contain a diversified set of small RNA-guided pathways that control genes, repeated sequences, and viruses at the transcriptional and posttranscriptional levels. Genome-wide profiles and analyses of small RNAs, particularly the large class of 24-nucleotide (nt) short interfering RNAs (siRNAs), were done for wild-type Arabidopsis thaliana and silencing pathway mutants...
Resource Type:: Article
Full Text:: siRNAs Kristin D. Kasschau 1,2 , Noah Fahlgren 1,2,3 , Elisabeth J. Chapman 1,2,3 , Christopher M

Genome-wide profiling of Populus small RNAs

Creator:: Klevebring, Daniel, Street, Nathaniel R., Fahlgren, Noah, Kasschau, Krisitin D., Carrington, James C., Lundeberg, Joakim, and Jansson, Stefan
Abstract:: Background: Short RNAs, and in particular microRNAs, are important regulators of gene expression both within defined regulatory pathways and at the epigenetic scale. We investigated the short RNA (sRNA) population (18-24 nt) of the transcriptome of green leaves from the sequenced Populus trichocarpa using a concatenation strategy in combination with...
Resource Type:: Article
Full Text:: ; Nathaniel R Street - nathaniel.street@plantphys.umu.se; Noah Fahlgren - fahlgren@cgrb.oregonstate.edu

Identification of MIR390a precursor processing defective mutants in Arabidopsis by direct genome sequencing

Creator:: Cuperus, Josh T., Montgomery, Taiowa A., Fahlgren, Noah, Burke, Russell T., Townsend, Tiffany, Sullivan, Christopher M., and Carrington, James C.
Abstract:: Transacting siRNA (tasiRNA) biogenesis in Arabidopsis is initiated by microRNA (miRNA) –guided cleavage of primary transcripts. In the case of TAS3 tasiRNA formation, ARGONAUTE7 (AGO7)– miR390 complexes interact with primary transcripts at two sites, resulting in recruitment of RNA-DEPENDENT RNA POLYMERASE6 for dsRNA biosynthesis. An extensive screen for Arabidopsis mutants...
Resource Type:: Article
Full Text:: . Montgomerya,b, Noah Fahlgrena,b, Russell T. Burkeb, Tiffany Townsendb, Christopher M. Sullivanb,c, and James C

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

Creator:: Carbonell, Alberto, Fahlgren, Noah, Garcia-Ruiz, Hernan, Gilbert, Kerrigan B., Montgomery, Taiowa A., Nguyen, Tammy, Cuperus, Josh T., and Carrington, James C.
Abstract:: In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicer-independent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were...
Resource Type:: Article
Full Text:: ARGONAUTES Using Slicer-Defective MutantsW OA Alberto Carbonell,a Noah Fahlgren,a Hernan Garcia-Ruiz,a

The ERI-6/7 Helicase Acts at the First Stage of an siRNA Amplification Pathway That Targets Recent Gene Duplications

Creator:: Fischer, Sylvia E. J., Montgomery, Taiowa A., Zhang, Chi, Fahlgren, Noah, Breen, Peter C., Hwang, Alexia, Sullivan, Christopher M., Carrington, James C., and Ruvkun, Gary
Abstract:: Endogenous small interfering RNAs (siRNAs) are a class of naturally occuring regulatory RNAs found in fungi, plants, and animals. Some endogenous siRNAs are required to silence transposons or function in chromosome segregation; however, the specific roles of most endogenous siRNAs are unclear. The helicase gene eri-6/7 was identified in the...
Resource Type:: Article
Full Text:: A. Montgomery1, Chi Zhang1, Noah Fahlgren2, Peter C. Breen1, Alexia Hwang1, Christopher M

Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

Creator:: Garcia-Ruiz, Hernan, Carbonell, Alberto, Hoyer, J. Steen, Fahlgren, Noah, Gilbert, Kerrigan B., Takeda, Atsushi, Giampetruzzi, Annalisa, Garcia Ruiz, Mayra T., McGinn, Michaela G., Lowery, Nicholas, Martinez Baladejo, Maria T., and Carrington, James C.
Abstract:: In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
Resource Type:: Article

Phytophthora Have Distinct Endogenous Small RNA Populations That Include Short Interfering and microRNAs

Creator:: Fahlgren, Noah, Bollmann, Stephanie R., Kasschau, Kristin D., Cuperus, Josh T., Press, Caroline M., Sullivan, Christopher M., Chapman, Elisabeth J., Hoyer, J. Steen, Gilbert, Kerrigan B., Grunwald, Niklaus J., and Carrington, James C.
Abstract:: In eukaryotes, RNA silencing pathways utilize 20-30-nucleotide small RNAs to regulate gene expression, specify and maintain chromatin structure, and repress viruses and mobile genetic elements. RNA silencing was likely present in the common ancestor of modern eukaryotes, but most research has focused on plant and animal RNA silencing systems. Phytophthora...
Resource Type:: Article
Full Text:: Small RNA Populations That Include Short Interfering and microRNAs Noah Fahlgren1,2, Stephanie R

High-Throughput Sequencing of Arabidopsis microRNAs : Evidence for Frequent Birth and Death of MIRNA Genes

Creator:: Fahlgren, Noah, Howell, Miya D., Kasschau, Kristin D., Sullivan, Christopher M., Cumbie, Jason S., Givan, Scott A., Law, Theresa F., Grant, Sarah R., Dangl, Jeffrey L., and Carrington, James C.
Abstract:: In plants, microRNAs (miRNAs) comprise one of two classes of small RNAs that function primarily as negative regulators at the posttranscriptional level. Several MIRNA genes in the plant kingdom are ancient, with conservation extending between angiosperms and the mosses, whereas many others are more recently evolved. Here, we use deep...
Resource Type:: Article
Full Text:: microRNAs: Evidence for Frequent Birth and Death of MIRNA Genes Noah Fahlgren1,2, Miya D. Howell1, Kristin D

Computational and analytical framework for small RNA profiling by high-throughput sequencing

Creator:: Fahlgren, Noah, Sullivan, Christopher M., Kasschau, Krisitin D., Chapman, Elisabeth J., Cumbie, Jason S., Montgomery, Taiowa A., Gilbert, Sunny D., Dasenko, Mark, Backman, Tyler W. H., Givan, Scott A., and Carrington, James C.
Abstract:: The advent of high-throughput sequencing (HTS) methods has enabled direct approaches to quantitatively profile small RNA populations. However, these methods have been limited by several factors, including representational artifacts and lack of established statistical methods of analysis. Furthermore, massive HTS data sets present new problems related to data processing and...
Resource Type:: Article
Full Text:: framework for small RNA profiling by high-throughput sequencing NOAH FAHLGREN,1,2 CHRISTOPHER M. SULLIVAN

Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline

Creator:: Gu, Weifeng, Shirayama, Masaki, Conte Jr., Darryl, Vasale, Jessica, Batista, Pedro J., Claycomb, Julie M., Moresco, James J., Youngman, Elaine, Keys, Jennifer, Stoltz, Matthew J., Chen, Chun-Cheih G., Chaves, Daniel A., Duan, Shenghua E., Kasschau, Krisitin D., Fahlgren, Noah, Yates III, John R., Mitani, Shohei, Carrington, James C., and Mello, Craig C.
Abstract:: Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the dicer-related helicase, drh-3, that abrogate both RNA interference and...
Resource Type:: Article
Full Text:: , Noah Falgren6, John R. Yates III4, Shohei Mitani7, James C. Carrington6, and Craig C. Mello1,2

ScholarsArchive@OSU

Genome-Wide Profiling and Analysis of Arabidopsis siRNAs

Genome-wide profiling of Populus small RNAs

Identification of MIR390a precursor processing defective mutants in Arabidopsis by direct genome sequencing

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

The ERI-6/7 Helicase Acts at the First Stage of an siRNA Amplification Pathway That Targets Recent Gene Duplications

Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

Phytophthora Have Distinct Endogenous Small RNA Populations That Include Short Interfering and microRNAs

High-Throughput Sequencing of Arabidopsis microRNAs : Evidence for Frequent Birth and Death of MIRNA Genes

Computational and analytical framework for small RNA profiling by high-throughput sequencing

Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline

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