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Filtering by: Creator Gallagher, M. Brett Academic Affiliation Botany and Plant Pathology

Sequence verification of synthetic DNA by assembly of sequencing reads

Creator:

Wilson, Mandy L., Cai, Yizhi, Hanlon, Regina, Taylor, Samantha, Chevreux, Bastien, Setubal, João C., Tyler, Brett M., and Peccoud, Jean

Abstract:

Gene synthesis attempts to assemble user-defined DNA sequences with base-level precision. Verifying the sequences of construction intermediates and the final product of a gene synthesis project is a critical part of the workflow, yet one that has received the least attention. Sequence validation is equally important for other kinds of...

Resource Type:

Article

Full Text:

Chevreux2, João C. Setubal1, Brett M. Tyler3 and Jean Peccoud1,4,* 1Virginia Bioinformatics Institute