Two new methods, one of broad specificity and the other of
narrow specificity, were developed for the identification of
agrobacteria. The former method was based on ribosomal antigens
that were common to members of the closely related Agrobacterium
and Rhizobium genera. Antisera to unwashed 50 S ribosomal subunits
from five...
Crown gall was found in numerous pecan orchards in Georgia. In
some instances, 60% of the trees were diseased. Galled trees were
less vigorous than noninfected trees. Among the pathogenic
Agrobacterium strains isolated from 18 galled trees from six
counties, biovar 1 strains predominated and most were sensitive to
agrocin...
A study was made of compounds produced by Agrobacterium radiobacter
strain 84 that are inhibitory to some pathogenic strains of A.
tumefaciens. Agrocins (inhibitory compounds) were partially purified
from large volumes of A. radiobacter strain 84 culture supernatants to
determine (i) if more than one agrocin is produced, (ii) if...
One hundred seventy-eight Agrobacterium isolates, 120 United
States (U.S.), 26 Australian and 32 of miscellaneous origin, were
characterized for their reactions to 20 biochemical and physiological
tests. Isolate pathogenicity and degree of host specificity, utilization
of nopaline and octopine, and sensitivity to agrocin-84 also were
determined. The U.S. isolates were...
In a recent survey, it was observed that many woody plant species grown in Pacific Northwest nurseries exhibited disease symptoms typical of a bacterial infection and Pseudomonas syringae was commonly isolated from these tissues. The distribution of the ice nucleation phenotype among P. syringae strains recovered from these infected woody...
The in planta growth of three wild type strains of phytopathogenic bacteria and three nonpathogenic mutant strains was studied to determine if mutations eliminating the ability of the mutant strains to cause disease had affected their growth in leaf tissue. The mutant strains were obtained by Tn5 mutagenesis of Pseudomonas...
A comparative study was made of Pinus ponderosa Laws, and
Arceuthobium campylopodum Engelm. f . campylopodum to determine
(1) whether or not the parasite or parasitized tissues accumulate
abnormal concentrations of minerals, (2) whether or not there
was evidence of a blockage of mineral translocation in the phloem
because of...