Index Catalog // ScholarsArchive@OSU

Batch and Continuous Flow Column Studies of Methane Consumption and Methanol Production by Methylosinus Trichosporium OB3b and Methylomicrobium Buryatense 5GB1 Immobilized In Ca-Alginate and Agarose Hydrogels

Creator:: Molzahn, Paige
Abstract:: Two methanotrophs, M. trichosporium OB3b and M. buryatense 5GB1, were encapsulated using two methods to investigate the potential of methane conversion for biofuel production. Ca-alginate and low melt agarose were used to immobilize the methanotrophs for batch and continuous flow column testing. Varying protein concentrations, residence times, and immobilization methods...
Resource Type:: Masters Thesis
Full Text:: AN ABSTRACT OF THE THESIS OF Paige

Experimental demonstration and modeling of aerobic cometabolism of 1,4-dioxane by isobutane-utilizing microorganisms in aquifer microcosms

Creator:: Rolston, Hannah M.
Abstract:: 1,4-dioxane, a probable human carcinogen at low (< 1ppb) concentrations, has emerged as a groundwater contaminant due to its historical use as a stabilizer for the chlorinated solvent 1,1,1-trichloroethane. Aerobic cometabolism, the use of a primary substrate to induce the production of microbial enzymes that fortuitously degrade other compounds, is...
Resource Type:: Masters Thesis
Full Text:: mg/L, indicating primary substrate inhibition. At a concentration of 200 µg/L, TCE did not inhibit

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Creator:: Laurance, Jon C.
Abstract:: Chlorinated solvents are common groundwater pollutants that often exist as mixtures resulting from the use of multiple solvents and their transformation into chlorinated daughter products. These sites are often co-contaminated with the solvent stabilizer 1,4-dioxane (1,4-D), which is not treated by traditional chlorinated solvent remediation techniques. Both 1,4-D and chlorinated...
Resource Type:: Masters Thesis
Full Text:: transformed to below 0.35 µg/L in several of the microcosms. The treated groundwater was exchanged for

Cometabolism of Chlorinated Ethenes by Burkholderia vietnamiensis G4 Grown on Aromatic Substrates: Resting and Co-encapsulated Cell Kinetic Tests

Creator:: Saito, Alyssa M.
Abstract:: The cometabolic ability of Burkholderia vietnamiensis G4 grown on aromatic substrates was first assessed by resting suspended cells, and subsequently for cells encapsulated with and without slow release compounds (SRCs). In Chapter 3, benzyl alcohol was assessed as a food-grade growth substrate for promoting cometabolism of trichloroethene (TCE), via the...
Resource Type:: Masters Thesis
Full Text:: suggest that benzyl alcohol inhibition occurs, but at concentrations greater than 10 mg/L and to a

Transformation of Phenanthrene by Mycobacterium sp. ELW1 and the Formation of Toxic Metabolites

Creator:: Schrlau, Jill E.
Abstract:: The ability of Mycobacterium sp. ELW1, a novel microbe capable of alkene oxidation, to co-metabolize phenanthrene (PHE) was studied. ELW1 was able to completely co-metabolize PHE, at different concentrations below its water solubility limit, in an aqueous environment. The alkene monooxygenases in ELW1, used to initiate oxidation of PHE, were...
Resource Type:: Masters Thesis
Full Text:: students who started this journey with me, and their significant others, (Hannah, Paige, Mark, Kristin

Development of a Paper-Based Whole Blood Phenylalanine Assay for PKU Diagnosis and Monitoring in Low Resource Settings

Creator:: Robinson, Robert Tyler
Abstract:: Phenylketonuria (PKU) is a genetic inborn metabolic disorder which inhibits the functional production of the enzyme phenylalanine hydroxylase (PAH). It currently affects one in 15,000 Americans and is the most common amino acid metabolism error found in newborns. Individuals affected with PKU are incapable of metabolizing the amino acid, phenylalanine...
Resource Type:: Masters Thesis
Full Text:: , grandmother Susan Pons and late grandfather Robert L. Pons. They all played instrumental roles in building

Graduate Teaching Assistants (GTAs) in a Studio Learning Environment: Practices, Epistemologies, and Pedagogical Development

Creator:: Smith, Christina
Abstract:: Providing transformational learning opportunities for undergraduate students demands changes to teaching practices. In large-enrollment introductory courses, graduate teaching assistants (GTAs) play an important role in facilitating student learning in small group environments. However GTAs are not provided the pedagogical development necessary to support them to be effective in their teaching...
Resource Type:: Dissertation

Engineering an Ex-Vivo System to Study Transport Phenomena in the Human Intervertebral Disc

Creator:: Shalash, Ward
Abstract:: Back pain is the leading cause of disability worldwide, entailing a significant socioeconomic impact. A primary source of back pain can be attributed to intervertebral disc (IVD) degeneration allowing nerve ingrowth, facet joint arthritis, disc bulging, and osteophyte formations that press on nearby nerve roots or the spinal cord. While...
Resource Type:: Dissertation
Full Text:: pressure. The osmolarity in the NP can range between 300 mOsm/L (hypoosmotic) to 496 mOsm/L

Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol

Creator:: Fuchs, Brian K.
Abstract:: Methane is a flammable gas that is the main component of natural gas. It is a highly potent greenhouse gas, and accounts for about 20% of greenhouse gas emissions. Methane is routinely flared in many industrial processes without harnessing any of its energy. The environmental impact and wasted energy potential...
Resource Type:: Masters Thesis
Full Text:: . Paige Molzahn performed experiments on cell activity in the gel and also helped me harvest cells on

Design of a microstructured liquid-liquid separator utilizing a capillary pressure gradient

Creator:: Zoebelein, Conor M.
Abstract:: Continuous liquid-liquid two-phase separation is needed in microfluidic devices. Due to the small characteristic lengths of microreactors, phenomena such as mass transport via molecular diffusion occur within seconds. Typically, discrete slugs of an extract are created within microchannels for high specific interfacial area to achieve intensified mass transfer. A challenge...
Resource Type:: Masters Thesis
Full Text:: , Tanner Bushnell, Paige Sanna-Molzahn, Davis Weymann, Sam Brannon, Dan Huang, Kevin Puac, Dr. Martin

ScholarsArchive@OSU

Batch and Continuous Flow Column Studies of Methane Consumption and Methanol Production by Methylosinus Trichosporium OB3b and Methylomicrobium Buryatense 5GB1 Immobilized In Ca-Alginate and Agarose Hydrogels

Experimental demonstration and modeling of aerobic cometabolism of 1,4-dioxane by isobutane-utilizing microorganisms in aquifer microcosms

Bioaugmentation of Pure and Enriched Cultures Fed Multiple Primary Substrates to Aerobically Cometabolize a Mixture of Chlorinated Solvents and 1,4-dioxane in Microcosms Containing Groundwater from a Department of Defense Site

Cometabolism of Chlorinated Ethenes by Burkholderia vietnamiensis G4 Grown on Aromatic Substrates: Resting and Co-encapsulated Cell Kinetic Tests

Transformation of Phenanthrene by Mycobacterium sp. ELW1 and the Formation of Toxic Metabolites

Development of a Paper-Based Whole Blood Phenylalanine Assay for PKU Diagnosis and Monitoring in Low Resource Settings

Graduate Teaching Assistants (GTAs) in a Studio Learning Environment: Practices, Epistemologies, and Pedagogical Development

Engineering an Ex-Vivo System to Study Transport Phenomena in the Human Intervertebral Disc

Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol

Design of a microstructured liquid-liquid separator utilizing a capillary pressure gradient

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