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Filtering by: Creator Chen, Kuei-Lin Academic Affiliation Chemistry Resource Type Part of Book

Protein Adducts of Aldehydic Lipid Peroxidation Products: Identification and Characterization of Protein Adducts Using an Aldehyde/Keto Reactive Probe in Combination with Mass Spectrometry

Creator:

Maier, Claudia S., Chavez, Juan, Wang, Jing, and Wu, Jianyong

Abstract:

This chapter describes a mass spectrometry-based strategy that facilitates the unambiguous identification and characterization of proteins modified by lipid peroxidation-derived 2-alkenals. The approach employs a biotinylated hydroxyl amine derivative as an aldehyde/keto reactive probe in conjunction with selective enrichment and tandem mass spectrometric analysis. Methodological details are given for model...

Resource Type:

Part of Book

Full Text:

rise to cyclopentenone-A2- and J2-isoprostanes (Chen et al., 1999), while eicosapentaenoic acid (EPA