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Filtering by: Creator Chavez, Juan Academic Affiliation Chemistry

Mass spectrometry-based identification and characterization of protein and peptide adducts of lipoxidation-derived aldehydes

Creator:

Chavez, Juan D.

Abstract:

Oxidative stress is recognized as an important underlying factor in the pathogenesis of many degenerative diseases as well as normal senescence. The free radicals, reactive oxygen species (ROS) and electrophiles produced during oxidative stress are capable of modifying nucleic acids, lipids and proteins. There are a variety of oxidative modifications...

Resource Type:

Dissertation

Full Text:

AN ABSTRACT OF THE DISSERTATION OF Juan D. Chavez for the degree of Doctor of Philosophy in
Protein Adducts of Aldehydic Lipid Peroxidation Products: Identification and Characterization of Protein Adducts Using an Aldehyde/Keto Reactive Probe in Combination with Mass Spectrometry

Creator:

Maier, Claudia S., Chavez, Juan, Wang, Jing, and Wu, Jianyong

Abstract:

This chapter describes a mass spectrometry-based strategy that facilitates the unambiguous identification and characterization of proteins modified by lipid peroxidation-derived 2-alkenals. The approach employs a biotinylated hydroxyl amine derivative as an aldehyde/keto reactive probe in conjunction with selective enrichment and tandem mass spectrometric analysis. Methodological details are given for model...

Resource Type:

Part of Book

Full Text:

REACTIVE PROBE IN COMBINATION WITH MASS SPECTROMETRY By CLAUDIA S. MAIER, JUAN CHAVEZ, JING WANG