Index Catalog // ScholarsArchive@OSU

Bacillus subtilis genome editing using ssDNA with short homology regions

Creator:: Wang, Yang, Weng, Jun, Waseem, Raza, Yin, Xihou, Zhang, Ruifu, and Shen, Qirong
Abstract:: In this study, we developed a simple and efficient Bacillus subtilis genome editing method in which targeted gene(s) could be inactivated by single-stranded PCR product(s) flanked by short homology regions and in-frame deletion could be achieved by incubating the transformants at 42 degrees C. In this process, homologous recombination (HR)...
Resource Type:: Article
Full Text:: . Microbiol. Methods, 34, 183–191. 59. Cao,G.Q., Zhang,X.H., Zhong,L. and Lu,Z.X. (2011) A modified electro

Metabolomic Profiling of Natural Products from Two Tolypocladium Fungal Species as Part of An Evolutionary Genomics Study

Creator:: Dickey, Bailey J.
Abstract:: This thesis describes an investigation of the natural products chemistry of two fungal species of the genus Tolypocladium. Natural products are small organic molecules that are considered non-essential for cell growth and reproduction, and thus part of secondary metabolism. Chemical profiling of these secondary metabolites using a combination of high-performance...
Resource Type:: Masters Thesis
Full Text:: : ______________________________________________________ Kerry L. McPhail This thesis describes an investigation

Application of modern NMR techniques to structure elucidation of bioactive natural products from tunicates

Creator:: Sikorska, Justyna
Abstract:: Recent developments in NMR hardware have extended the reach of natural products chemists to structure elucidation of compounds isolated on a nanomolar scale. In parallel with advances in hardware new NMR techniques for structure elucidation have evolved, e.g. quantitative NOEs, residual dipolar couplings (RDCs)and diffusion-ordered spectroscopy (DOSY). The application of...
Resource Type:: Dissertation
Full Text:: from Tunicates Abstract approved: Kerry L. McPhail Recent developments in NMR hardware have

Drug Discovery and Chemical Ecology Investigations of Specialized Metabolism in Tolypocladium and Paraisaria

Creator:: Tehan, Richard M.
Abstract:: Since the discovery of penicillin in 1928, the fungal kingdom has remained a fertile source for the discovery of new, biologically active, natural products. Further, as natural products continue to be discovered from fungi, a deeper understanding of the critical role of specialized metabolism in fungal ecology is afforded. The...
Resource Type:: Dissertation
Full Text:: : ______________________________________________________ Kerry L. McPhail Since the discovery of penicillin in 1928, the fungal kingdom has

Natural Products Discovery from Unique Microbial Habitats

Creator:: Gallegos, David A.
Abstract:: Marine natural products possess an abundance of diverse chemical scaffolds with unique biological activities. By targeting unusual or unique microbial environments within varying marine ecologies we can continue to discover novel chemistry with potentially new molecular targets. The research presented here is focused on exploring unusual chemical ecologies and the...
Resource Type:: Dissertation
Full Text:: Habitats. Abstract approved: ______________________________________________________ Kerry L

Investigation of unique marine environments for microbial natural products

Creator:: Thornburg, Christopher C.
Abstract:: Metagenomics has revealed that the marine microbial biosphere is immensely more diverse than originally considered, and is an almost untapped reservoir for the potential discovery of microbial natural products. Despite numerous advances in culturing, biosynthetic engineering and genomic-based screening efforts to uncover much of this diversity in relatively accessible environments,...
Resource Type:: Dissertation
Full Text:: Microbial Natural Products Abstract approved: Kerry L. McPhail Metagenomics has revealed that the

Regulation of Redox Homeostasis in Skin Cells for the Prevention of UV-Induced Stress and Treatment of Melanoma

Creator:: Carpenter, Evan L.
Abstract:: Melanocytes are dendritic, pigment-producing cells located in the skin and are responsible for its protection against the deleterious effects of solar ultraviolet radiation (UVR), which include DNA damage and elevated reactive oxygen species (ROS). They do so by synthesizing photoprotective melanin pigments and distributing them to adjacent skin cells (e.g.,...
Resource Type:: Dissertation
Full Text:: . Ralph L. Reed, and Dr. Gitali Ganguli-Indra performed the experiments. Dr. Cristobal Miranda and Dr

Advanced studies on cyclic amino acids in neurological signaling and peptide antibiotics

Creator:: Blanchard, David, L.
Abstract:: L-pipecolic acid (L-PA) is the higher homolog of proline. It occurs naturally in many organisms, including primates, as an intermediate in lysine degradation. The pathway by which lysine is converted into L-pipecolic acid employs the enzyme Lpipecolate oxidase (L-PO), and appears to be tissue specific to the central nervous system...
Resource Type:: Dissertation
Full Text:: OF David L. Blanchard for the degree of Doctor of Philosophy in Pharmacy presented on March

Molecular genetics and enzymology of secondary metabolite biosynthesis. I, Isolation of natural product biosynthesis gene clusters from symbiotic marine organisms. II, Enzymology of blasticidin S biosynthesis

Creator:: Grochowski, Laura L.
Abstract:: Molecular genetic and enzymological techniques have been employed to study secondary metabolite biosynthesis. These investigations have focused on two projects: the cloning and heterologous expression of biosynthetic gene clusters from unculturable marine organisms and the characterization of individual enzymes involved in the biosynthesis of the antifungal agent blasticidin S. The...
Resource Type:: Dissertation
Full Text:: AN ABSTRACT OF THE DISSERTATION OF Laura L. Grochowski for the

Characterization of the Mechanisms of Action and Molecular Targets of Hop-derived Flavonoids in the Mitigation of Obesity-related Metabolic Impairments

Creator:: Paraiso, Ines L.
Abstract:: Obesity is a complex multifactorial disease resulting from excessive energy intake compared to energy expenditure. The excess energy is mostly stored as fat in enlarged adipocytes, but some lipids may infiltrate other organs such as the liver to form ectopic fat. Adipose tissue and ectopic fat produce and secrete a...
Resource Type:: Dissertation
Full Text:: General Introduction Ines L. Paraiso 3 General introduction Obesity, a public health

ScholarsArchive@OSU

Bacillus subtilis genome editing using ssDNA with short homology regions

Metabolomic Profiling of Natural Products from Two Tolypocladium Fungal Species as Part of An Evolutionary Genomics Study

Application of modern NMR techniques to structure elucidation of bioactive natural products from tunicates

Drug Discovery and Chemical Ecology Investigations of Specialized Metabolism in Tolypocladium and Paraisaria

Natural Products Discovery from Unique Microbial Habitats

Investigation of unique marine environments for microbial natural products

Regulation of Redox Homeostasis in Skin Cells for the Prevention of UV-Induced Stress and Treatment of Melanoma

Advanced studies on cyclic amino acids in neurological signaling and peptide antibiotics

Molecular genetics and enzymology of secondary metabolite biosynthesis. I, Isolation of natural product biosynthesis gene clusters from symbiotic marine organisms. II, Enzymology of blasticidin S biosynthesis

Characterization of the Mechanisms of Action and Molecular Targets of Hop-derived Flavonoids in the Mitigation of Obesity-related Metabolic Impairments

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