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Filtering by: Advisor Siemens, Philip J. Remove constraint Advisor: Siemens, Philip J. Academic Affiliation College of Pharmacy Remove constraint Academic Affiliation: College of Pharmacy

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  • Biosynthetic studies of fungal diterpene antibiotics

    Creator:
    Yao, Qingmei
    Abstract:
    The isoprenoid pathway is one of the major biosynthetic pathways of secondary metabolites. Isoprenoids with a C₂₀ skeleton are known as diterpenoids and are derived from the common precursor, geranylgeranyl diphosphate (GGPP). This dissertation describes approaches to discover the diterpene synthase enzymes which provide the backbone structures for pleuromutilin and...
    Resource Type:
    Masters Thesis
    Full Text:
    Abstract approved: __________________________________________ Philip J. Proteau The isoprenoid

  • Studies of non-mevalonate isoprenoid biosynthesis : the 1-deoxy-D-xylulose-5-phosphate isomeroreductase (DXR) mediated reaction

    Creator:
    Woo, Youn-Hi
    Abstract:
    This dissertation details the investigation of an alternate pathway to isoprenoids that occurs in plants and microorganisms, the non-mevalonate pathway. This exploration of the pathway focuses on the second step, the conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methylerythritol-4-phosphate (MEP) by the enzyme DXP isomeroreductase (DXR). These studies led to an appreciation...
    Resource Type:
    Dissertation
    Full Text:
    OH [ 0 OH j NADPH OH OH jOPO32 DXP 2-C-methylerythrose -4-phosphate MEP Fig.l.7 The proposed

  • Studies on 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Synechocystis sp. PCC6803 : characterization of mutants and inhibitors

    Creator:
    Fernandes, Roberta P. M.
    Abstract:
    In recent years, the methyl erythritol phosphate (MEP) pathway to isoprenoids has been the subject of intensive research. The interest is because isoprenoids have important roles in many cellular processes essential for the survival of several pathogenic organisms, making the inhibition of this pathway an attractive target for the drug...
    Resource Type:
    Dissertation

  • Studies on isoprenoid biosynthesis in Euglena gracilis

    Creator:
    Kim, Dojung
    Abstract:
    This dissertation describes studies of isoprenoid biosynthesis in the phytoflagellate Euglena gracilis. Elucidation of a novel isoprerioid pathway, the 2-Cmethyl-D-erythritol-4-phosphate (MEP) pathway, in addition to the generally known mevalonate (MVA) pathway, led to extensive exploration of the distribution of both pathways in a variety of organisms. To date, photosynthetic eukaryotes...
    Resource Type:
    Dissertation
    Full Text:
    thank my major Professor, Philip J. Proteau, for his support, guidance, and patience throughout my

  • Syntheses and evaluation of putative enzyme inhibitor of isoprenoid biosynthesis

    Creator:
    Phaosiri, Chanokporn
    Abstract:
    The discovery of the methylerythritol phosphate pathway (the MEP pathway) as an alternate pathway for isoprenoid biosynthesis in some organisms including most bacteria, malarial parasites and plants, but not in animals, has stimulated extensive studies in this area. Research has revealed the potential of finding novel antibacterials, antimalarial drugs, and...
    Resource Type:
    Dissertation
    Full Text:
    of Putative Enzyme Inhibitors of Isoprenoid Biosynthesis Abstract approved: Philip The discovery