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  • Development and optimization of a kinetics-based enzyme-linked immunosorbent assay (KELA) for serologic diagnosis of bovine respiratory syncytial virus infection

    Creator:
    Chaloupka, Kim A.
    Abstract:
    A kinetics-based enzyme-linked immunosorbent assay (KELA) for detection of serum IgG antibody against bovine respiratory syncytial virus (BRSV) was developed and optimized. Optimization factors tested included brand of microELISA plate, length of incubation periods at various temperatures, type of conjugate, purified versus precipitated antigen preparations, type of dilution buffer as...
    Resource Type:
    Masters Thesis
    Full Text:
    Donald E. Mattson, D.V.M., Ph.D. A kinetics-based enzyme-linked immunosorbent assay (KELA) for

  • Development of an enzyme-linked immunosorbent assay for the serologic diagnosis of bovine adenovirus type 3

    Creator:
    Whipple, Margaret Jo
    Abstract:
    An enzyme-linked immunosorbent assay was developed to measure specific antibody response in bovine sera to bovine adenovirus type 3 (BA3), an etiologic agent of respiratory disease causing economic losses annualy to the cattle industry. Observed endpoint titers were determined using the intersection point from optical density values of serially diluted...
    Resource Type:
    Masters Thesis
    Full Text:
    . Abstract approved: Redacted for Privacy Donald E. Mattson An enzyme-linked immunosorbent assay was

  • Serologic survey of llamas in Oregon for antibodies to viral diseases of livestock

    Creator:
    Picton, Rebecca Anne
    Abstract:
    Serums from 270 llamas representing 21 farms throughout Oregon were obtained and assayed for antibody levels against viruses of livestock. These viral diseases included: bovine viral diarrhea (BVD), bovine herpesvirus 1 (BHV-1), parainfluenza-3 (PI-3), bovine respiratory syncytial virus (BRSV), bovine adenovirus species 3 (BA3), equine herpesvirus 1 (EHV-1), equine adenovirus...
    Resource Type:
    Masters Thesis
    Full Text:
    Abstract approved: Redacted for Privacy Donald E. Mattson Serums from 270 llamas representing 21 farms

  • Antigenic comparison of bovine, ovine, equine, and llama adenoviruses

    Creator:
    Yusuf, Irwandi
    Abstract:
    Fifteen adenoviruses from cattle, sheep, horses, and llamas were studied by virus neutralization to determine their degree of antigenic similarity. Prototype viruses included bovine adenoviruses species 1-8, ovine adenoviruses species 5 and 6, and equine adenovirus species 1. Unclassified viruses that were compared to the prototype viruses were isolated from...
    Resource Type:
    Masters Thesis
    Full Text:
    ADENOVIRUS ES Abstract approved: Donald E. Mattson, D.V.M., Ph.D. Fifteen adenoviruses from cattle, sheep

  • Viruses associated with respiratory disease : a study of sheep in an Oregon and Colorado feedlot

    Creator:
    Babar, Shakeel
    Abstract:
    A study was undertaken to determine the prevalence of virus infection in lambs undergoing respiratory tract disease (RTD). The investigation was divided into three study groups and consisted of lambs which were 4 to 5 months of age. In the first group (N=200), paired serum samples were taken from lambs...
    Resource Type:
    Masters Thesis
    Full Text:
    Privacy Abstract approved: Donald E. Mattson, D.V.M., Ph.D. A study was undertaken to determine the

  • Characterization of adenovirus isolated from sheep in Oregon

    Creator:
    Babar, Shakeel
    Abstract:
    Six 3 to 4 weeks old, cesarian-derived lambs were inoculated with ovine an adenovirus isolate 475N. Inoculated lambs showed moderate clinical signs of respiratory distress, conjunctivitis, and loose feces during the 10-day observation period. Virus was detected from nasal and conjunctival swabs starting on postinoculation day (PID) 2. Virus was...
    Resource Type:
    Dissertation
    Full Text:
    America and for keeping me on the right Path. My special thanks are due to Dr. Mattson,Donald E. my