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Filtering by: Has journal Toxicology Letters Academic Affiliation Fisheries and Wildlife Has journal sim Journal of Aquatic Animal Health

Analysis of salmonid leukocytes purified by hypotonic lysis of erythrocytes

Creator:

Crippen, Tawni L., Bootland, Linda M., Leong, Jo-Ann C., Fitzpatrick, Martin S., Schreck, Carl B., and Vella, Anthony T.

Abstract:

A technique that uses hypotonic lysis of erythrocytes was optimized for the purification of leukocytes from the peripheral blood and anterior kidney (pronephros) of rainbow trout Oncorhynchus mykiss. Comparisons of initial blood dilution (1:2, 1:4, and 1:6) and the time of exposure to hypotonic conditions (10, 20, and 40 s)...

Resource Type:

Article

Full Text:

- mined in vitro by morphological alteration and neu- tral red absorption. Toxicology Letters 24:119–124