Radish anthocyanin extract has potential as a natural colorant because of
its pigment stability and attractive red hue. Presence of undesirable aroma
compounds could limit its applications in foods. The pungent principle of
radish, 4-methylthio-3-butenyl isothiocyanate (MTBI), is produced
enzymatically upon cell injury from its glucosinolate precursor, 4-methylthio-3-butenyl glucosinolate (MTBG),...
The pigments of blackberry fruit were investigated by analytical and preparative high performance liquid chromatography (HPLC) to characterize anthocyanin patterns in commercially important blackberry (Rubus eubatus) cultivars and selections. Five anthocyanin pigments were detected in Evergreen blackberry and they were identified as cyanidin-3-glucoside, cyanidin-3-rutinoside, pentosecyanidin derivative and two acylated cyanidin...
Seven red raspberry (Rubus Ideaus L.) samples from Oregon, Washington, British Columbia and New Zealand were processed into juice. These, along with three juice samples supplied from West Germany were analyzed by high performance liquid chromatography, (HPLC), for nonvolatile acid, sugar, anthocyanin and anthocyanidin composition. In
order to quantitate the...
The distribution of total phenolics, antioxidant capacity, monomeric anthocyanin, and ascorbic acid contents in the peel and flesh of Red Delicious, Granny Smith, and Fuji apples during a six-month storage period were determined. In addition, the effect of 1-MCP (1-Methyl cyclopropene) on these parameters of the edible portion of apples...
Fruit juice concentrates- hard pear, soft pear, fig,
prune, raisin, white grape, and pineapple, and sweeteners-invert
beet, invert cane, and high fructose corn syrup
(HFCS) were characterized by sugar profiles, nonvolatile
acid profiles, UV spectral profiles, and mineral content.
These fruit juices and sweeteners were also used to
adulterate commercial...
A method for separation and characterization of individual anthocyanins was
developed. High Performance Liquid Chromatography (HPLC) with a polymer based
reversed-phase column was used to separate the pigments while on-line Photodiode
Array Detection (PDA) was employed to record the UV and Visible spectrum of the
individual peaks. Spectral information obtained...
Frozen Marion (Rubus sp. hyb) and Evergreen blackberries (Rubus laciniatus) (whole berries, berries without seeds, and seeds alone) were acetone/ chloroform extracted. Phenolics were isolated using C₁₈ cartridges and polyamide resins, then characterized and measured by reversed-phase HPLC/diode array spectral techniques. The Polyamide isolated fractions of Marion berries contained four...
The distribution of anthocyanin pigments and polyphenolics of sweet (Prunus avium) and sour cherries (Prunus cerasus) were determined by Ultraviolet- Visible (UV-Visible) spectrophotometry and High Performance Liquid Chromatography with photodiode array detector (HPLC-DAD). Their antioxidant properties were determined by Oxygen Radical Absorbance Capacity (ORAC) and Ferric Reducing Antioxidant Power (FRAP)....
This investigation was conducted to study the effects of
blanching and storage temperatures on the color of strawberry
juice and concentrate. Strawberry juice and concentrate (42°βrix)
were prepared from blanched crushed Tioga strawberries. Crushed
strawberries were blanched, in a steam-jacketed kettle, at 70°,
80°, and 90°C for 1 min. Ascorbic...
Pectic substances in the cell wall have long thought to
be responsible for the textural changes in fruit during
ripening and senescence. Compositional variation within
the pectic macromolecules could affect the external
characteristics of the fruit. Strawberries undergo adverse
quality changes such as softening and juice loss after
freezing and...
Fresh ripe, cantaloupes were processed into juice and
juice concentrate. Processing trials were conducted on
fresh and frozen fruits with and without rind. The effects
of maceration enzymes and fining agents on yield and quality
were investigated. Compositional measurement included
°Brix, pH, titratable acidity, formol values, ascorbic acid
content, total...
Apples growing in New Zealand, Argentina, Mexico, Michigan and
Washington were used to provide the compositional data and to
develop the analytical procedures for the use in verification of
authenticity of apple juices.
High Performance Liquid Chromatography (HPLC) techniques were
applied for glucose, fructose, sucrose, sorbitol, malic, citric,
quinic, shikimic,...
Different concentrations of various browning inhibitors and a firming agent were evaluated to prevent browning and softening of Bartlett, Bosc and Anjou pear slices during cold storage at 2-4°C. Browning inhibition effect of Semperfresh, Snow Fresh and cysteine were compared with that of 4-hexylresorcinol (4-HR). The effect of ripeness on...
The influence of cultivar and maturity on polyphenolic composition and
antioxidant activity was investigated by UV-Visible spectrophotometry and
analytical high performance liquid chromatography (HPLC) in commercially
important blackberry cultivars and selections. The anthocyanin pigments changed
tremendously with ripening. Total anthocyanins increased from 74.7-317 mg/100g
fw from underripe to over ripe...
Methodology for characterization of the phenolic profile of
apple, pear and grape juice was developed. It utilized High
Performance Liquid Chromatography (HPLC) for separation of
individual phenolic compounds and Diode Array Detection for
recording the Ultra-violet (UV) spectrum of chromatographic
peaks. The high resolution achieved and the ability of recording...
The phenolic profile of pineapple juice was analyzed by reverse-phase HPLC/Diode Array Detector, and 9 major peaks accounting for 70% of total peak area were characterized. None of these peaks are represented by the pineapple phenolics reported to be present by previous workers. Most of the major peaks are not...
Red radish anthocyanin extract (RAE) was investigated for coloring brined
cherries as an alternative to FD&C Red No. 40. Red radish (Raphanus sativus L.)
anthocyanins were extracted from liquid nitrogen powdered epidermal tissue using
acetone, partitioned with chloroform, and isolated using C-18 resin. The monomeric
anthocyanin content was determined by...
Wines were made from thawed, frozen red raspberries and blackberries
(Meeker and Evergreen varieties, respectively) by 1. fermentation on the
pulp, 2. fermentation of depectinized juice, and 3. fermentation of high
temperature short time (HTST) treated depectinized juice. The influence
of fining was also investigated. The wines were stored at...
Maintaining the structural integrity of cell walls
largely determines the texture of fruit products during
freezing processes. Strawberries undergo major textural
changes when frozen, and varietal differences in quality
after thawing are not readily predicted from mechanical
and sensory testing of the fresh fruit. The objectives of
this study were...
This study was conducted to evaluate the influence of maturity, mold contamination and variety on the color quality of strawberry wines. Indices for acceptability were determined using instrumental measurements and sensory analysis. Compositional analyses were done on the fully ripe and overripe Benton and Totem strawberries. Included were anthocyanin content,...
Red radish (Raphanus sativus L.) anthocyanins were extracted from liquid
nitrogen powdered epidermal tissue using acetone and chloroform and isolated using
C-18 resin. Chemical structure and conformation of major pigments were elucidated
by 1-D and 2-D NMR, Mass Spectroscopy, HPLC, and spectral analyses. Two novel
di-acylated anthocyanins, pelargonidin 3-0-[2-0-(β-D-glucopyranosyl)-(6-0- trans-p-coumaroyl-...
Frozen blueberries were pilot-plant-processed into juice and concentrate by 2 treatments (heat and SO2), and a control. Qualitative, and quantitative anthocyanin and polyphenolic content were monitored after each processing step. Major losses occurred after the initial processing steps. Juice processing by-product contained a substantial amount of anthocyanins and polyphenolics (>42%...
Chipping varieties and model systems were used to determine the role of potato
constituents on chip color. Composition was evaluated by HPLC and chip color
measured using a ColorQuest colorimeter. Reducing sugar (RS) content did not
completely explain color quality when present in low concentrations (<60 mg/lOOg).
Levels of ascorbic...
The purpose of this.investigation was to determine how enzymes and oxygen influence the role ascorbic acid has on pigment degradation and browning during storage for strawberry juice and concentrate.
The ascorbic and dehydroascorbic acid content of blanched and unblanched, nitrogen-treated and air-exposed samples of strawberry juice and concentrate was measured...
Pelargonidin 3-glucoside (pgd 3-glu), pelargonidin 3-sophoroside (pgd 3-soph),
and pelargonidin 3-sophoroside 5-glucoside acylated with cinnamic and malonic acids
(acyl-pgd 3-soph 5-glu) were extracted from strawberries (Fragaria anannassa cv,
Totem), nasturtium flowers (Tropaeolum majus), and radish peel (Raphanus sativus L.
cv, fuego), respectively. Their stability was studied in natural and model...
Two methods of separating sugars and acids of Bartlett pears
were compared to decide upon a working method for later studies
involving identification and quantitation of sugars and acids during
ripening of pears and in the production and storage of pear concentrate.
The first method involved precipitation of acids with...