Substrate and inhibitor specificities have been used to classify
esterases. The purpose of this work was to determine the substrate
and inhibitor specificities of the esterases in an aquous extract of
lyophilyzed carrots.
Esterase activity was determined manometrically at 37°C. The
optimum pH of the carrot esterases with several substrates...
This investigation was conducted to differentiate the esterases
in a water extract of freeze-dried green beans on the basis of inhibitor
and substrate specificities. An attempt was made to classify
these esterases according to the criteria used for animal esterases.
Activity of the esterases was measured manometrically using
the Gilson...
Esterase activity of an aqueous extract of the green bean was
separated into fourteen bands, while aqueous extracted pea esterases
revealed seven bands, by polyacrylamide-gel electrophoresis. The
fourteen bands of bean esterase activity formed three groups; slow,
intermediate and fast moving. α-Naphthyl acetate, propionate, and
n-butyrate and AS naphthol acetate...
Proanthocyanidins are a grape-derived phenolic class of compound that provide wine with bitterness and astringency. Because of this, proanthocyanidins are considered to be essential components of wine quality. Proanthocyanidins are localized in the seed, skin and stem tissue of the grape berry and the composition of proanthocyanidins will vary depending...
Using phenolic scavengers different methods were examined to determine the optimum conditions for extracting polyphenol oxidase (PPO) from d'Anjou pears. Efficiency of the extraction was evaluated based on browning of the extract, PPO activity per g of pear tissue, absorption scan of 410 nm to -240 nm, and the isozyme...
Seafood, defined here as marine and freshwater fish and shellfish, is recognized as a healthy food choice because it is a low-fat protein source that provides long-chain omega-3 fatty acids important for early development along with eye and heart health. However, seafood is also known to contain certain contaminants, such...
Monoterpenes are important volatile compounds to the aroma of many white wines. Many monoterpenes are chiral and found in wine as different isomers. These isomers (also known as enantiomers) are non-superimposable mirror images of each other, maintain the same molecular structure and in many instances possess different sensory characteristics. Much...
Stability, substrate and inhibitor specificity and electrophoretic
properties of a crude polyphenol oxidase (PPO) preparation extracted
from d'Anjou pears (Pyrus communis L.) were investigated. Levels
of polyvinylpyrrolidone and pH of buffer for extraction were found
to affect the specific activity of the extracted enzyme. An extract
prepared with 1.5 g...