Shoot regeneration in vitro is a difficult problem for cherry species. This study addressed three approaches to the problem, regeneration of adventitious shoots from roots and leaves of mature cherry clones, and from cotyledons of open-pollinated 'Royal Ann' cotyledons. Regeneration on roots occurred at a very low frequency. Adventitious shoots...
Alternating temperature (22°C / -1°C) pretreatments were effective for
increasing cold hardiness and recovery of cryopreserved meristems. Combining low
temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the
time required for optimal cold hardiness of meristems. Desiccation tolerance was
directly correlated with increased cold hardiness and recovery of...