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Analysis of salmonid leukocytes purified by hypotonic lysis of erythrocytes

Creator:

Crippen, Tawni L., Bootland, Linda M., Leong, Jo-Ann C., Fitzpatrick, Martin S., Schreck, Carl B., and Vella, Anthony T.

Abstract:

A technique that uses hypotonic lysis of erythrocytes was optimized for the purification of leukocytes from the peripheral blood and anterior kidney (pronephros) of rainbow trout Oncorhynchus mykiss. Comparisons of initial blood dilution (1:2, 1:4, and 1:6) and the time of exposure to hypotonic conditions (10, 20, and 40 s)...

Resource Type:

Article

Full Text:

, resulting from cell debris and release of nuclear material, was gently pulled from the top of the leukocyte