Index Catalog // ScholarsArchive@OSU

The distribution and influence of sterols in yeast membranes

Creator:: Bottema, Cynthia D. K.
Abstract:: Plasma membranes from Saccharomyces cerevisiae were prepared by a new procedure involving lyticase treatment of the yeast cells. The plasma membranes were right-side out, closed vesicles of uniform appearance with a sterol to phospholipid molar ratio of 0.365. The thermotropic behavior of these plasma membranes from wild-type yeast and from...
Resource Type:: Dissertation
Full Text:: membranes as the cisternae of Golgi apparatus in mouse pancreatic cells (73) and the nuclear membranes of

Control of macromolecular synthesis in Saccharomyces cerevisiae

Creator:: Wehr, Carl Timothy
Abstract:: Synthesis of total RNA, DNA, and protein was followed in a haploid yeast auxotroph during starvation for required amino acids and uracil, and during shift-up and shift-down conditions. During amino acid starvation, synthesis of macromolecular constituents was not immediately affected, reflecting the presence of large intracellular amino acid pools. Under...
Resource Type:: Dissertation
Full Text:: INTRODUCTION 1 REVIEW OF LITERATURE 3 MATERIALS AND METHODS 23 Cultures 23 Growth Conditions 23 Measurement

Aspects of ergosterol biosynthesis in Saccharomyces cerevisiae

Creator:: Monner, David Anthony
Abstract:: The regulation of ergosterol biosynthesis in yeast has been studied. The system has been examined for the presence of an end-product inhibition. Both anaerobically and aerobically grown cells have been examined for this effect. A feedback inhibition of sterol synthesis has been shown, but it appears to be a secondary...
Resource Type:: Dissertation
Full Text:: REVIEW OF LITERATURE 3 MATERIALS 14 METHODS 15 Cultures 15 Media 15 Anaerobic growth of cells 16

A study of the function and physiological forms of ergosterol in Saccharomyces cerevisiae

Creator:: Adams, Bruce Gordon
Abstract:: A water-soluble complex containing ergosterol together with a component of yeast has been isolated. The complex can be isolated from commercial yeast extract to which ergosterol has been added or directly from whole yeast cells. The complexing agent from yeast extract is also capable of solubilizing cholesterol and a long...
Resource Type:: Dissertation
Full Text:: respiratory deficient organisms as a result of either a nuclear mutation or through the loss of a

The role of S-adenosylmethionine in the biosynthesis of ergosterol and related sterols in Saccharomyces cerevisiae

Creator:: Turner, Jan Ross
Abstract:: An enzyme system was prepared from Saccharomyces cerevisiae, strain MCC, which carries out the transfer of the methyl group from S-adenosylmethionine or methionine to the side chain of yeast sterols. The cell-free system has been shown to be incapable of synthesizing ergosterol but carries out the synthesis of two as...
Resource Type:: Dissertation
Full Text:: Page INTRODUCTION 1 REVIEW OF LITERATURE 3 MATERIALS AND METHODS 10 Cultures 10 Media 10

S-adenosylmethionine : [triangle]²⁴-sterol methyltransferase and the regulation of ergosterol biosynthesis in Saccharomyces cerevisiae

Creator:: Bailey, Richard B.
Abstract:: The inhibition of S-aden.osylmethionine: Δ²⁴-sterol methyltransferase (EC 2.1.1.41) activity by endogenous cellular components has been studied in vitro. The principal inhibitors were Na⁺ and K⁺; Cs⁺, NH₄⁺ and Li⁺ were also shown to inhibit the reaction. The inhibition by potassium cation has been studied, and we have found that energy...
Resource Type:: Dissertation
Full Text:: MATERIALS AND METHODS 17 Organism and Cultural Conditions 17 Enzyme Preparation 17 Isolation of Mitochondria

Aspects of the regulation of methyl group formation in methionine biosynthesis by Saccharomyces cerevisiae

Creator:: Nakamura, Kenji Dennis
Resource Type:: Dissertation
Full Text:: reading of this thesis. TABLE OF CONTENTS Page INTRODUCTION 1 REVIEW OF LITERATURE MATERIALS AND

The development of Phytophthora cactorum as a model system for the study of sterol metabolism

Creator:: Gonzales, Robert A.
Abstract:: Phytophthora cactorum lacks sterol synthesis capability. Nevertheless, it exhibits vegetative growth in the total absence of sterol. Supplementation of the growth medium with sterol, however, resulted in a three-fold increase in cell yield. This increase was shown by whole cell and cell free experiments to be the consequence of altered...
Resource Type:: Dissertation
Full Text:: of organic solvent extractable material (52). The substance was was found to be a component of a

Regulation of the terminal reactions for methionine biosynthesis in yeast

Creator:: Spence, Kemet Dean
Abstract:: Studies were carried out to elucidate the mechanism of enzymatic control of methionine biosynthesis in Saccharomyces cerevisiae. Enzymatic studies demonstrated that, in addition to the methionine activating enzyme, the S-adenosylmethionine:homocysteine transmethylase enzyme was induced during cultivation in the presence of excess methionine. A similar, though reduced effect was observed when...
Resource Type:: Dissertation
Full Text:: . . . . . . . . . . . . . TABLE OF CONTENTS Page INTRODUCTION 1 REVIEW OF LITERATURE 4 MATERIALS AND METHODS 11 Cultures

The physiological effects of an antimycotic azasterol in Saccharomyces cerevisiae

Creator:: Hays, Phillip Roy
Resource Type:: Dissertation
Full Text:: . D. Pierce, Jr., for their Nuclear Magnetic Resonance studies of ergosta-8,14-dien-3a-ol, and Dr. G

ScholarsArchive@OSU

The distribution and influence of sterols in yeast membranes

Control of macromolecular synthesis in Saccharomyces cerevisiae

Aspects of ergosterol biosynthesis in Saccharomyces cerevisiae

A study of the function and physiological forms of ergosterol in Saccharomyces cerevisiae

The role of S-adenosylmethionine in the biosynthesis of ergosterol and related sterols in Saccharomyces cerevisiae

S-adenosylmethionine : [triangle]²⁴-sterol methyltransferase and the regulation of ergosterol biosynthesis in Saccharomyces cerevisiae

Aspects of the regulation of methyl group formation in methionine biosynthesis by Saccharomyces cerevisiae

The development of Phytophthora cactorum as a model system for the study of sterol metabolism

Regulation of the terminal reactions for methionine biosynthesis in yeast

The physiological effects of an antimycotic azasterol in Saccharomyces cerevisiae

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