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Filtering by: Alternative title ssim AFS 62 Academic Affiliation Microbiology Decade 1980-1989

Purification and characterization of a DNA polymerase gamma from human trophoblast tissue

Creator:

Wong, Scott W.

Abstract:

DNA polymerase gamma has been purified over 67,500-fold from human trophoblast tissue. Purification was achieved through successive chromatographies on phosphocellulose, DEAE-cellulose, DNA-cellulose, and high pressure gel exclusion through Fractogel TSK HW55. This preparation yielded a specific activity of 81 units/mg of protein using the preferred template-primer, poly (A)-oligo (dT)₁₂₋₁₃. Analysis...

Resource Type:

Masters Thesis

Full Text:

activity is associated structurally with DNA polymerase a. has come from a number of studies (62-64). Kozu
Molecular cloning of infectious pancreatic necrosis virus and characterization of the coding capacity of the complementary DNA

Creator:

Huang, Manley T. F.

Abstract:

The two segments of double-stranded RNA (dsRNA) from infectious pancreatic necrosis virus (IPNV) were cloned into plasmid vector pUC8. Two distinct sets of overlapping clones were identified by restriction enzyme and Southern blot analysis. Each of these sets was shown, by Northern blot analysis, to be exclusively related to either...

Resource Type:

Dissertation