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  • Monoclonal antibodies to 5'-deoxy-5' methylthioadenosine phosphorylase

    Creator:
    Parker, Ricardo Jerome
    Abstract:
    5'-Deoxy-5'-methylthioadenosine phosphorylase catalyzes the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine to form 5'-deoxy-5'-methylthioribose-1-phosphate and adenine in mammalian cells and plays an important role in methionine and purine salvage. The enzyme is abundant in normal tissues and in cell lines derived from normal cells. However, several malignant tissues and cell lines have been...
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    Dissertation
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    deficiency, I employed hybridoma technology to produce a series of monoclonal IgM anti-bovine

  • Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances

    Creator:
    Dukovcic, Stephanie R.
    Abstract:
    Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application of current detection methods by reporting on the toxicity of a sample and the potential to cause disease. Previously, Betta splendens erythrophores have been described...
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    Dissertation
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    response of a living cell to sense biologically stimulating agents. This emerging technology extends the

  • Harnessing Heterogeneity in Human Gut Microbiome Data

    Creator:
    Tataru, Christine
    Abstract:
    The focus of this thesis is to design, characterize, and apply novel computational methods and molecular systems to interrogate heterogeneous human gut microbiome-related phenomena. In Chapter 2, I design, implement, and characterize a method for embedding co-occurrence patterns derived from massive 16s amplicon datasets. I use this method to 1....
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    Dissertation
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    behavior and metabolism. This research exists in the space between computer science, microbiology, and

  • Interactions between microbial dynamics, water flow, and solute transport in unsaturated porous media

    Creator:
    Yarwood, Rockie R.
    Abstract:
    Bioremediation in the vadose zone is unpredictable because of poor understanding of factors influencing microbial growth in this environment. A lab-scale experimental system was developed to examine, noninvasively, interactions between microbial growth, water flow, and solute transport in unsaturated porous media. Measurements of microbial colonization, and its impact on hydrology,...
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    . Financial support for this work was provided by the National Science Foundation (grant #: 9630293) and the

  • Secreted Agrobacterium rhizogenes Effector Protein GALLS Interacts with Arabidopsis Host Proteins and Stimulates Agrobacterium-mediated Transformation

    Creator:
    Wei, Wei
    Abstract:
    Agrobacterium species transform plant cells by targeting a portion of plasmid-encoded bacterial DNA to the host nucleus. Genetic transformation by A. tumefaciens and A. rhizogenes requires secreted effector proteins. The tumor-inducing (Ti) plasmid from virulent A. tumefaciens encodes VirE2, a secreted single-stranded DNA-binding protein required for efficient transformation of hosts....
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    Dissertation
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    , biotechnology, and plant science, plant responses to Agrobacterium are not well-understood. Genetic

  • Transcriptional regulation of Rhizobium meliloti nitrogen fixation genes

    Creator:
    Evans, Paul D.
    Abstract:
    The transcriptional promoter sequences for the Rhizobium meliloti nitrogen fixation genes nifA and nifB were cloned to a β-galactosidase gene fusion plasmid vector and transferred by homologous recombination to a specialized transducing phage. The promoter fusions were then transduced to a recombination deficient strain of Escherichia coli as single-copy lysogens...
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    , devotion, and patience. I have served this canon seven years or more, yet am no nearer science than before

  • Recombinant vaccines against infectious hematopoietic necrosis virus : bacterial systems for vaccine production and delivery

    Creator:
    Simon, Benjamin E.
    Abstract:
    Several systems were examined for the production and delivery of recombinant vaccines for fish. C. crescentus was employed to produce a fragment of the IHNV glycoprotein. When administered by injection to 0.5 gram rainbow trout (Oncorhynchus mykiss), one of the fusion proteins (184 amino acids of the IHNV glycoprotein fused...
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    Dissertation
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    NA76RG0476), and by the Canadian National Science and Engineering Research Council Technology Partnership

  • Fecal Bacteroidetes host distributions and environmental source tracking

    Creator:
    Dick, Linda K.
    Abstract:
    Contamination of recreational and shellfish waters with fecal pollution is a major water quality issue with associated economic impacts and human health risks. Reliable fecal source identification and rapid, quantitative analyses are essential components of risk assessment. Enteric bacteria that are endemic to specific hosts have a potential role as...
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    . 1999. Emerging marine diseases-- climate links and anthropogenic factors. Science. 285: 1505-15 10

  • Chemical and Genetic Investigations of Toxic Freshwater Cyanobacterial Blooms for Improved Monitoring and Understanding of Lake Ecology

    Creator:
    Collart, Lindsay P.
    Abstract:
    Toxin production by cyanobacterial harmful algal blooms (cyanoHABs) in freshwater systems has lasting ecological and human impacts. Nutrients, light availability, hydrology, and microbial community composition impact the frequency and intensity of toxic cyanoHABs. Climate change will exacerbate toxic cyanoHABs, making real time and predictive monitoring a vital tool for managing...
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    Dissertation

  • Bacterial pathogens and associated toxins involved in erythrophore cell aggregation

    Creator:
    Hutchison, Janine R.
    Abstract:
    Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable, they cannot evaluate the toxicity of a sample and the potential to cause disease. Previously, erythrophore cells derived from Betta splendens had been suggested as...
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    for giving me the opportunity to work in her lab. Her enthusiasm and love of science is contagious