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Filtering by: Has journal Journal of Nuclear Materials Remove constraint Has journal: Journal of Nuclear Materials Academic Affiliation Microbiology Remove constraint Academic Affiliation: Microbiology Degree Level Doctoral Remove constraint Degree Level: Doctoral

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  • Isolation and characteristics of the DNA from nuclear polyhedrosis virus

    Creator:
    Carnegie, John William
    Abstract:
    The genome of the nuclear polyhedrosis virus which infects the larvae of the tussock moth, the lepidopteran, Hemerocampa pseudotsugata, was isolated, identified as deoxyribonucleic acid, and characterized in terms of its physical and biological properties. An isolation procedure for the viral DNA was established which consisted of the following steps:...
    Resource Type:
    Dissertation
    Full Text:
    , ethylenediaminetetraacetic acid, sodium chloride 3 to be the genetic material of different polyhedrosis viruses. Nuclear

  • Host cell nuclear involvement in vaccinia virus replication

    Creator:
    Villarreal, Elcira C.
    Abstract:
    It was previously shown that vaccinia virus is unable to complete its replicative cycle in cells that have been subjected to cytochalasin-B mediated enucleation prior to infection. Likewise, in the presence of the drug α-amanitin, a potent inhibitor of host RNA polymerase II but not of viral transcription, the replication...
    Resource Type:
    Dissertation
    Full Text:
    REPLICATING INDEPENDENTLY OF THE HOST CELL NUCLEUS 8 Summary 9 Introduction 10 Methods and Materials 13

  • The association of DNA with other cellular materials in Escherichia coli lysates

    Creator:
    Porter, Bruce Wallace
    Abstract:
    The association of other materials with DNA has been studied extensively in higher organisms and more recently in bacteria. However, investigation of these complexes in bacteria have for the most part been restricted to the study of a specific function this association may serve, or a specific kind of association....
    Resource Type:
    Dissertation
    Full Text:
    role in the function and metabolism of the genetic material. The first materials found associated

  • Significance of pigments in radiation resistance of Micrococcus radiodurans

    Creator:
    Krabbenhoft, Kenneth Louis
    Abstract:
    An ecological study of a microorganism similar to that isolated in 1956 and designated as Micrococcus radiodurans indicated that the organism could be isolated from several different environments. By taking advantage of its radio-resistivity, high levels of gamma radiation were used in the isolation procedures to inactivate most of the...
    Resource Type:
    Dissertation
    Full Text:
    material. All of the environments tested were in the vicinity of the Nebergall Packing Plant, Albany

  • Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water

    Creator:
    Brooks, Lauren
    Abstract:
    High counts of fecal indicators, used to signal the potential presence of pathogens associated with untreated waste, result in the classification of water bodies throughout the United States as impaired. Nonpoint sources of unknown origin that contribute to fecal contamination make management of impaired waters challenging, as they are difficult...
    Resource Type:
    Dissertation
    Full Text:
    decay, studies using ruminant sources of fecal material are underrepresented in the available data

  • Characterization of the Oregon sockeye salmon virus

    Creator:
    Wingfield, William Harry
    Abstract:
    Characterization of a virus requires that its physical, biochemical and biological properties be determined. In this study the Oregon sockeye salmon virus was characterized. It was isolated in the fall of 1958 from juvenile sockeye salmon, Oncorhynchus nerka, being reared at the Oregon Fish Commission's Willamette River Hatchery. When inoculated...
    Resource Type:
    Dissertation
    Full Text:
    . First there was a thickening of the nuclear membrane accompanied by alteration of the nucleoli. This

  • Factors affecting the Ceratomyxa shasta infectious cycle and transmission between polychaete and salmonid hosts

    Creator:
    Bjork, Sarah J.
    Abstract:
    Ceratomyxa shasta is a myxozoan parasite that infects salmonid fishes causing the disease ceratomyxosis that is characterized by severe hemorrhage and necrosis of the intestine and death of the fish host. Ceratomyxa shasta is endemic to the Pacific Northwestern United States and Canada, where epizootics are reported for both wild...
    Resource Type:
    Dissertation
    Full Text:
    immunological techniques. Journal of Fish Diseases 12, 509-522. Bartholomew, J.L., Whipple, M.J., Stevens

  • Molecular genetic analysis of vaccinia virus genes which confer resistance to alpha-amanitin

    Creator:
    Tamin, Azaibi
    Abstract:
    Several laboratories have shown that the VV replication cycle is dependent on the host cell nucleus. The basic biological observation is that if the host cell nucleus is physically removed by cytochalasin B-mediated enucleation, functionally inactivated by UV-irradiation, or selectively blocked with a-amanitin, the results are the same: W is...
    Resource Type:
    Dissertation
    Full Text:
    (1986) Journal of Virology 57: 65-70]. In an attempt to understand the genetic organization of this

  • Isolation and characterization of a leukocytolytic factor from Aeromonas salmonicida

    Creator:
    Fuller, David Wallace
    Abstract:
    The purpose of this study was to find what effect temperature and immunization has on the phagocytic activity of fish leukocytes against certain disease causing bacteria. Also proposed was the isolation and characterization of a leukocytolytic factor produced by one of these bacteria, Aeromonas salmonicida. Investigations revealed that temperature had...
    Resource Type:
    Dissertation
    Full Text:
    . salmonicida produced more of this substance than an avirulant strain. This material was demon- strated to be

  • A molecular study of viral proteins in the pathogenesis of infectious hematopoietic necrosis virus

    Creator:
    Chiou, Pinwen Peter
    Abstract:
    The role of viral proteins in the pathogenesis of infectious hematopoietic necrosis virus (IHNV) was studied at the molecular level. The expression of the viral genes at the protein and RNA level, and their cellular localization, were characterized to further our understanding of viral pathogenesis. The pathogenic effect of individual...
    Resource Type:
    Dissertation
    Full Text:
    of host-directed gene expression at the level of transcription and induction of nuclear fragmentation