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Filtering by: Has journal Food Chemistry Remove constraint Has journal: Food Chemistry Academic Affiliation Microbiology Remove constraint Academic Affiliation: Microbiology Degree Name Doctor of Philosophy (Ph.D.) Remove constraint Degree Name: Doctor of Philosophy (Ph.D.)

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  • Improved detection methods and physiological studies of staphylococcal enterotoxin

    Creator:
    Kimble, Charles E.
    Abstract:
    The purpose of this study was to develop means to facilitate the detection of staphylococcal enterotoxin and to elucidate factors that influence enterotoxin formation. It was found that semipurification flat-gel electrophoresis in a water-cooled apparatus was more efficient than CM-cellulose and Sephadex column chromatography currently used to separate toxin from...
    Resource Type:
    Dissertation
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    Sephadex column chroma- tography currently used to separate toxin from soluble food constitu- ents. The

  • Ecophylogenetic Characterization of the Gut Microbiome of At-risk Companion and Food Animals

    Creator:
    Kirchoff, Nicole S.
    Abstract:
    Agricultural and companion animals are integral to our way of life as they provide us with nourishment, financial resources, transportation, and companionship. However, pets, managed animals in the agricultural industry, as well as wild populations of food animals are all subject to health and mortality risks. The gut microbiome is...
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    Dissertation
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    : Ecophylogenetic Characterization of the Gut Microbiome of At-risk Companion and Food Animals Abstract

  • Studies on the inhibition of food-borne pathogens and spoilage bacteria by lactic acid starter cultures

    Creator:
    Daly, Charles
    Abstract:
    The ability of Streptococcus diacetilactis to inhibit a variety of food-borne pathogens and spoilage organisms in milk and broth cultures was demonstrated. Test organisms inhibited included Pseudomonas and Alcaligenes species, Eschericia coli, Salmonella, Staphylococcus aureus, Clostridium perfringens and Vibrio parahaemolyticus. In general, approximately 99.0% and 99.9% inhibition was observed in...
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    Dissertation
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    Studies on the inhibition of food-borne pathogens and

  • Detection of staphylococcal enterotoxin A by Laurell electroimmunodiffusion

    Creator:
    Gasper, Ekkehart
    Abstract:
    The serological detection of staphylococcal enterotoxin A by the quantitative technique of Laurell electroimmunodiffusion is described as well as the application of the technique to the detection of small quantities of enterotoxin A in foods. High dilutions of type-specific rabbit antiserum were used in 1% agarose gels, 1 mm thick,...
    Resource Type:
    Dissertation
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    investigated. Dairy as well as non- dairy food items were artificially contaminated with purified staphylo

  • Human health significance of vibrios from the aquatic environment

    Creator:
    Nishibuchi, Mitsuaki
    Abstract:
    For the enrichment and enumeration of Vibrio fluvialis, a broth medium was designed by modifying alkaline peptone (AP) medium. This new V. fluvialis enrichment medium (FEM) was shown to be more effective than AP medium in field samplings where a total of 177 samples (estuarine waters and sediment, sewage, and...
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    Dissertation
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    various protein chemistry techniques, and specific antiserum to VVA was prepared. Using anti-VVA serum, a

  • The Influence of Microbial Communities on Spatiotemporal Elemental Cycling in Coastal Margins Revealed by Community- and Population-level Genomics and Proteomics

    Creator:
    Kieft, Brandon P.
    Abstract:
    Biotic and abiotic processes at continent-ocean interfaces cycle a disproportionate mass of carbon and nutrients relative to their global surface area, and microbial activity is a principal determinant of organic and inorganic matter flux in these transition zones. Most studies using modern high-throughput ‘omics techniques to link microorganisms with costal...
    Resource Type:
    Dissertation
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    food webs during the cold and wet season of low primary productivity on the central Oregon coast

  • Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances

    Creator:
    Dukovcic, Stephanie R.
    Abstract:
    Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application of current detection methods by reporting on the toxicity of a sample and the potential to cause disease. Previously, Betta splendens erythrophores have been described...
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    Dissertation
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    agents such as pesticides, chemicals, purified bacterial toxins and food-associated bacteria. The

  • Quantifying Carbon Production in Marine Ecosystems Using Expressions of Phytoplankton Physiology

    Creator:
    Penta, William Bryce
    Abstract:
    Phytoplankton initiate the marine carbon cycle by fixing carbon dioxide into biologically available compounds. These abundant single celled organisms mediate carbon flux from the atmosphere to the deep ocean and are the base of the marine food web, supplying new carbon to higher trophic levels. Phytoplankton are highly diverse and...
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    Dissertation
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    atmosphere to the deep ocean and are the base of the marine food web, supplying new carbon to higher

  • Studies on growth metabolites produced by Propionibacterium shermanii

    Creator:
    Salih, Mohammed Ali
    Abstract:
    The ability of Propionibacterium shermanii to produce growth metabolites which were inhibitory to food spoilage indicator organisms was investigated. Asperqillus niqer, a Pseudomonas species and Kluyveromyces fraqilis were the spoilage indicators chosen to represent molds, gram negative psychrotrophic bacteria and yeasts, respectively. Supplementation of milk or whey based media was...
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    Dissertation
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    shermanii to produce growth metabolites which were inhibitory to food spoilage indicator organisms was

  • Bacterial pathogens and associated toxins involved in erythrophore cell aggregation

    Creator:
    Hutchison, Janine R.
    Abstract:
    Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable, they cannot evaluate the toxicity of a sample and the potential to cause disease. Previously, erythrophore cells derived from Betta splendens had been suggested as...
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    Dissertation
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    detection method for the model food-associated pathogenic bacteria Salmonella enteritidis, Clostridium