Index Catalog // ScholarsArchive@OSU

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

Creator:: Kaller, Margo
Abstract:: Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...
Resource Type:: Masters Thesis
Full Text:: ability. As artificial mimics of DNA molecules, compounds in the antisense oligonucleotide family are

Organelle trafficking of chimeric ribozymes and genetic manipulation of mitochondria

Creator:: Val, Romain, Wyszko, Eliza, Valentin, Clarisse, Szymanski, Maciej, Cosset, Anne, Alioua, Malek, Dreher, Theo W., Barciszewski, Jan, and Dietrich, Andre
Abstract:: With the expansion of the RNA world, antisense strategies have become widespread to manipulate nuclear gene expression but organelle genetic systems have remained aside. The present work opens the field to mitochondria. We demonstrate that customized RNAs expressed from a nuclear transgene and driven by a transfer RNA-like (tRNA-like) moiety...
Resource Type:: Article
Full Text:: With the expansion of the RNA world, antisense strategies have become widespread to manipulate nuclear

Host-pathogen Interactions of Respiratory RNA Viruses and the Application of Recombinase Polymerase Amplification for Viral Diagnostics

Creator:: Prescott, Meagan A.
Abstract:: Humans and viral disease are inextricably intertwined. Viral disease plays an immeasurable role in human life, from the disease and economic burden associated with every facet of contending with human viral disease, to managing the consequences of viral disease in organisms important to our food supply, economy, and entertainment. The...
Resource Type:: Dissertation
Full Text:: purposes. PPMO antisense technology was employed to target and suppress the expression of the host gene

Investigations of regulatory T cell induction by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin during a graft-versus-host response

Creator:: Marshall, Nikki B.
Abstract:: The immune toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been studied for over 35 years, but only recently has the profound immune suppression associated with TCDD exposure been linked to induction of regulatory T cells (Tregs). The effects of TCDD are mediated through binding the aryl hydrocarbon receptor (AhR), a ligand-activated transcription...
Resource Type:: Dissertation
Full Text:: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Chapter 3: Cell-Penetrating Peptides Facilitate Delivery of Antisense Oligomers into

Virus resistance in transgenic plants expressing translatable and untranslatable forms of the tobacco etch virus coat protein gene sequence

Creator:: Lindbo, John A.
Resource Type:: Dissertation
Full Text:: Oligonucleotide purification: ethanol precipitation method. 183 VI.2 Phosphorylation of oligonucleotides 184 VI.3

The bacteriophage P1 bof protein : a corepressor whose action can both negatively and positively affect phage gene expression

Creator:: Schaefer, Timothy S.
Resource Type:: Dissertation
Full Text:: "repressor" has been shown to be an antisense RNA (8). This RNA binds to a homologous region on the ant mRNA

Preventing crown gall disease using oncogene silencing or VirE1-expressing transgenics

Creator:: Humann, Jodi L.
Abstract:: Crown gall disease is caused by the ubiquitous soil bacterium Agrobacterium tumefaciens which transfers a portion if DNA (T-DNA) into the plant cell. Preventing infection by using the biocontrol strain Agrobacterium radiobacter K84 is currently the only defense for crown gall. Two different resistance strategies were examined in this work....
Resource Type:: Dissertation
Full Text:: inserting a 20-bp self-complementary oligonucleotide (5'-AGCTGGCGCGCCGGC GCGCC-3') with Hindlil-compatible

Characterization of interferon and retroposon-like repetitive elements in salmonid fish

Creator:: Tengelsen, Leslie A.
Abstract:: Hatchery-reared salmonid fish routinely encounter stress due to handling, barging, tagging, and overcrowding. It has been demonstrated that there exists a direct correlation between stress and transient immune suppression which can last for many days in fish. Epizootic viral infections routinely appear in hatcheries and can have a devastating effect...
Resource Type:: Dissertation

Roles of brca2 (fancd1) in Oocyte Nuclear Architecture, Gametogenesis, Gonad Tumors, and Genome Stability in Zebrafish

Creator:: Rodriguez-Mari, Adriana, Wilson, Catherine, Titus, Tom A., Canestro, Cristian, BreMiller, Ruth A., Yan, Yi-Lin, Nanda, Indrajit, Johnston, Adam, Kanki, John P., Gray, Erin M., He, Xinjun, Spitsbergen, Jan, Schindler, Detlev, and Postlethwait, John H.
Abstract:: Mild mutations in BRCA2 (FANCD1) cause Fanconi anemia (FA) when homozygous, while severe mutations cause common cancers including breast, ovarian, and prostate cancers when heterozygous. Here we report a zebrafish brca2 insertional mutant that shares phenotypes with human patients and identifies a novel brca2 function in oogenesis. Experiments showed that...
Resource Type:: Article
Full Text:: structural similarity (Figure 1E). The DBDs of zebrafish and human Brca2 contain three oligonucleotide

A molecular study of viral proteins in the pathogenesis of infectious hematopoietic necrosis virus

Creator:: Chiou, Pinwen Peter
Abstract:: The role of viral proteins in the pathogenesis of infectious hematopoietic necrosis virus (IHNV) was studied at the molecular level. The expression of the viral genes at the protein and RNA level, and their cellular localization, were characterized to further our understanding of viral pathogenesis. The pathogenic effect of individual...
Resource Type:: Dissertation

ScholarsArchive@OSU

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

Organelle trafficking of chimeric ribozymes and genetic manipulation of mitochondria

Host-pathogen Interactions of Respiratory RNA Viruses and the Application of Recombinase Polymerase Amplification for Viral Diagnostics

Investigations of regulatory T cell induction by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin during a graft-versus-host response

Virus resistance in transgenic plants expressing translatable and untranslatable forms of the tobacco etch virus coat protein gene sequence

The bacteriophage P1 bof protein : a corepressor whose action can both negatively and positively affect phage gene expression

Preventing crown gall disease using oncogene silencing or VirE1-expressing transgenics

Characterization of interferon and retroposon-like repetitive elements in salmonid fish

Roles of brca2 (fancd1) in Oocyte Nuclear Architecture, Gametogenesis, Gonad Tumors, and Genome Stability in Zebrafish

A molecular study of viral proteins in the pathogenesis of infectious hematopoietic necrosis virus

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