Concentrations of polybrominated diphenyl ethers (PBDEs),
pesticides, polychlorinated biphenyls (PCBs), and polycyclic
aromatic hydrocarbons were measured in 136 fish from 14 remote
lakes in 8 western U.S. National Parks/Preserves between
2003 and 2005 and compared to human and wildlife contaminant
health thresholds. A sensitive (median detection limit, -18
pg/g wet...
Bacteriophage S-CRM01 has been isolated from a freshwater strain of Synechococcus and shown to
be present in the upper Klamath River valley in northern California and Oregon. The genome of this
lytic T4-like phage has a 178,563 bp circular genetic map with 297 predicted protein-coding genes
and 33 tRNA genes...
A retrovirus homologue gene of cellular cyclin D₁, walleye dermal sarcoma virus rv-cyclin
gene (orf A or rv-cyclin), was expressed in the livers of zebrafish under the control
of liver fatty-acid binding protein (lfabp) promoter. To prevent possible fatality caused
by over-expression of the oncogene, the GAL4/UAS system was used...
Ceratomyxa shasta is a myxozoan parasite identified as a contributor to salmon mortality in the Klamath River, USA. The parasite has a complex life cycle involving a freshwater polychaete, Manayunkia speciosa and a salmonid. As part of ongoing research on how environmental parameters influence parasite establishment and replication, we designed...
Surface disinfection of fertilized fish eggs is widely used in aquaculture to reduce
extraovum pathogens that may be released from brood fish during spawning, and this is routinely
used in zebrafish (Danio rerio) research laboratories. Most laboratories use approximately 25-50 ppm unbuffered chlorine solution for 5-10 min. Treatment of embryos...
An ecological study of a microorganism similar to that isolated
in 1956 and designated as Micrococcus radiodurans indicated
that the organism could be isolated from several different environments.
By taking advantage of its radio-resistivity, high levels of
gamma radiation were used in the isolation procedures to inactivate
most of the...
Comparatively little work has been done on bacterial lipids.
There are profound differences between bacterial lipids in general
and lipids of the higher forms of life in such respects as the absence
of sterols, phospholipids low in nitrogen but high in inositol
and carbohydrate, the presence of large proportions of...
The dairy industry relies primarily on consistent acid production
by the lactic streptococci for the manufacture of certain cheeses
and fermented dairy products. Variation in these cultures due to
genetic exchange has not been thoroughly investigated. This study
was undertaken to determine if genetic homology exists within the lactic
group,...
The terminal reactions in the biosynthetic pathway of methionine
in Saccharomyces cerevisiae were investigated in this study. Analyses
of a number of methionine auxotrophs for biochemical deficiences
established four mutant groups. It was observed from qualitative feeding
experiments that some auxotrophs were able to utilize S-adenosylmethionine
(AM) and S-adenosyl-homocysteine (AH)...
The public health and economic significance of rapid acid production
by lactic streptococci in controlled dairy fermentations is well
known. However, the fast acid-producing characteristic of these organisms
is not stable, and cultures of fast organisms have been shown
to contain slow cells. These studies were carried out to characterize...