Index Catalog // ScholarsArchive@OSU

Toxigenic and non-toxigenic strains of Clostridium botulinum type E : A. Differentiation by immunodiffusion : B. Attempts to induce toxigenicity by mutagenic agents

Creator:: Balstraz, Edwina Gene
Abstract:: In this study of toxigenic and non-toxigenic strains of Clostridium botulinum type E, attempts were made to induce toxin production in non-toxigenic cultures by exposure to mutagenic agents and to differentiate the organisms by immunodiffusion. First, cultures of non-toxic Cl. botulinum type E were exposed to various mutagenic agents in...
Resource Type:: Masters Thesis
Full Text:: agents AN ABSTRACT OF THE THESIS OF EDWINA GENE BALSTRAZ for the (Name of student) in Microbiology

Quorum sensing gene regulation in Pseudomonas aeruginosa

Creator:: Gupta, Rashmi
Abstract:: Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
Resource Type:: Dissertation
Full Text:: : Quorum Sensing Gene Regulation in Pseudomonas aeruginosa. Abstract approved: Martin Schuster

Preventing crown gall disease using oncogene silencing or VirE1-expressing transgenics

Creator:: Humann, Jodi L.
Abstract:: Crown gall disease is caused by the ubiquitous soil bacterium Agrobacterium tumefaciens which transfers a portion if DNA (T-DNA) into the plant cell. Preventing infection by using the biocontrol strain Agrobacterium radiobacter K84 is currently the only defense for crown gall. Two different resistance strategies were examined in this work....
Resource Type:: Dissertation
Full Text:: upstream of the nptll gene and in plant DNA flanking the T-DNA. KpnI-digested DNA from line BVE1/H

Characterization of Sporulation and Germination Genes in Clostridium perfringens

Creator:: Talukdar, Prabhat Kumar
Abstract:: Clostridium perfringens is a Gram-positive, anaerobic, spore-forming bacterium that can produce as many as 17 different toxins and are responsible to cause a wide array of gastrointestinal (GI) and histotoxic diseases in humans and animals. As individual strains produce a subset of these toxins, C. perfringens strains can be classified...
Resource Type:: Dissertation
Full Text:: L-cysteine and L-lysine at pH range of 5.5-7.0. qRT-PCR analyses with RNA extracted from 8 h

Regulation of late gene expression in vaccinia virus

Creator:: Weinrich, Scott L.
Abstract:: The prototypal poxvirus vaccinia is a cytoplasmic-replicating DNA virus which contains a large genome with the capacity to encode 200 polypeptides. Tight temporal regulation coordinates the expression of this large number of genes throughout a relatively short replication cycle. As a first step towards identifying and understanding the regulatory mechanisms...
Resource Type:: Dissertation
Full Text:: Regulation of late gene expression in vaccinia virus AN

Transcriptional regulation of Rhizobium meliloti nitrogen fixation genes

Creator:: Evans, Paul D.
Abstract:: The transcriptional promoter sequences for the Rhizobium meliloti nitrogen fixation genes nifA and nifB were cloned to a β-galactosidase gene fusion plasmid vector and transferred by homologous recombination to a specialized transducing phage. The promoter fusions were then transduced to a recombination deficient strain of Escherichia coli as single-copy lysogens...
Resource Type:: Dissertation
Full Text:: nifB were cloned to a 13-galactosidase gene fusion plasmid vector and transferred by homologous

Characterization of Baculovirus genes involved in genome replication and processing

Creator:: Vanarsdall, Adam L.
Abstract:: The Baculoviridae comprise a diverse group of occluded DNA viruses that contain large double-stranded DNA genomes of 80 - 180 kb and may encode up to 180 gene products. To understand how baculoviruses replicate and process their genomes and the gene products that are involved in these events, a series...
Resource Type:: Dissertation
Full Text:: that contain large double-stranded DNA genomes of 80 - 180 kb and may encode up to 180 gene

Automated and Accurate Estimation of Gene Family Abundance from Shotgun Metagenomes

Creator:: Nayfach, Stephen, Bradley, Patrick H., Wyman, Stacia K., Laurent, Timothy J., Williams, Alex, Eisen, Jonathan A., Pollard, Katherine S., and Sharpton, Thomas J.
Abstract:: Shotgun metagenomic DNA sequencing is a widely applicable tool for characterizing the functions that are encoded by microbial communities. Several bioinformatic tools can be used to functionally annotate metagenomes, allowing researchers to draw inferences about the functional potential of the community and to identify putative functional biomarkers. However, little is...
Resource Type:: Article
Full Text:: Automated and Accurate Estimation of Gene Family Abundance

Analysis of Bacteroidales 16S rRNA gene sequences from a geographically isolated human population

Creator:: Petersen, Carolyn A.
Abstract:: Standard methods of measuring fecal pollution in water do not distinguish between human and non-human sources. Molecular technology enabled the development of host-specific markers that distinguish fecal sources. Human specific PCR primers, HF183F and HF134F, were designed based on phylogenetic analyses of partial 16S rRNA gene sequences from the Bacteroidales...
Resource Type:: Masters Thesis
Full Text:: September 5, 2006. Title: Analysis of Bacteroidales 16S rRNA Gene Sequences from a Geographically

Molecular characterization of the MX genes of rainbow trout (Oncorhynchus mykiss)

Creator:: Trobridge, Grant David
Abstract:: The Mx protein family of rainbow trout (Oncorhynchus mykiss) was characterized at the molecular level. With primers derived from a partial sequence of Perch (Perca fluviatis) Mx genomic DNA, a partial rainbow trout Mx genomic DNA sequence was obtained by polymerase chain reaction (PCR) amplification. All salmonid fish investigated contained...
Resource Type:: Dissertation
Full Text:: ) Chapter Page 3. Characterization of a Rainbow Trout Mx Gene 25 Abstract 26 Introduction 26

ScholarsArchive@OSU

Toxigenic and non-toxigenic strains of Clostridium botulinum type E : A. Differentiation by immunodiffusion : B. Attempts to induce toxigenicity by mutagenic agents

Quorum sensing gene regulation in Pseudomonas aeruginosa

Preventing crown gall disease using oncogene silencing or VirE1-expressing transgenics

Characterization of Sporulation and Germination Genes in Clostridium perfringens

Regulation of late gene expression in vaccinia virus

Transcriptional regulation of Rhizobium meliloti nitrogen fixation genes

Characterization of Baculovirus genes involved in genome replication and processing

Automated and Accurate Estimation of Gene Family Abundance from Shotgun Metagenomes

Analysis of Bacteroidales 16S rRNA gene sequences from a geographically isolated human population

Molecular characterization of the MX genes of rainbow trout (Oncorhynchus mykiss)

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