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Filtering by: Creator Mercer, Dacey M. Remove constraint Creator: Mercer, Dacey M. Academic Affiliation Microbiology Remove constraint Academic Affiliation: Microbiology

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  • Identification of intracellular trafficking signals contained within the vaccinia virus F13L protein

    Creator:
    Honeychurch, Kady M.
    Abstract:
    The F13L protein is the major envelope antigen of vaccinia virus, the prototypic member of the Orthopoxvirus genus. F13L is 372 residues in length and is essential for the formation of wrapped forms of virus. F13L contains a number of potential functional domains including a palmitylation site, a phospholipase domain...
    Resource Type:
    Dissertation
    Full Text:
    DISSERTATION OF Kady M. Honeychurch for the degree of Doctor of Philosophy in Microbiology presented on

  • Quantification of Osmolytes in the Sargasso Sea Surface Layer Water Column

    Creator:
    Muslin, Omran
    Abstract:
    Oceanic carbon cycling plays a major role in determining global atmospheric CO₂. A better understanding of dissolved organic matter and its constituents in the water column and how it affects marine carbon cycling is sought after. This study successfully measured osmolytes, low molecular zwitterionic compounds derived from amino acids, in...
    Resource Type:
    Masters Thesis
    Full Text:
    weighed. This pellet is then re-suspended in nanopure water, water with18.2 MΩ ionic purity and little

  • Molecular genetic analysis of vaccinia virus genes which confer resistance to alpha-amanitin

    Creator:
    Tamin, Azaibi
    Abstract:
    Several laboratories have shown that the VV replication cycle is dependent on the host cell nucleus. The basic biological observation is that if the host cell nucleus is physically removed by cytochalasin B-mediated enucleation, functionally inactivated by UV-irradiation, or selectively blocked with a-amanitin, the results are the same: W is...
    Resource Type:
    Dissertation
    Full Text:
    HindIII N/M region of the genome by the use of marker rescue techniques [E.C. Villarreal and D.E. Hruby

  • Single-Cell Genomics of Uncultivated Marine Bacteria

    Creator:
    Landry, Zachary
    Abstract:
    Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
    Resource Type:
    Dissertation
    Full Text:
    ., Thrash, J.C., Schwalbach, M.S., Vergin, K.L., Landry, Z.C., Ellisman, M., Deerinck, T., Sullivan, M.B

  • Characterization of vaccinia virus A12L protein : its proteolysis and functional analyses in virus replication

    Creator:
    Yang, Su Jung
    Abstract:
    In order to produce infectious virus progeny, vaccinia virus (VV) undergoes morphogenic proteolysis to regulate the structural rearrangements of virus particles. Several of the major structural precursor proteins of VV are cleaved at a conserved Ala-Gly-X (where X is any amino acid) motif by the VV I7L core protein proteinase...
    Resource Type:
    Dissertation
    Full Text:
    (VanSlyke et al. 1991a, VanSlyke et al. 1991b, Whitehead and Hruby, 1994a, Mercer and Traktman, 2005

  • Impacts of microbial chemical interactions on marine plankton physiology

    Creator:
    Moore, Eric R.
    Abstract:
    The bioavailability of chemical compounds in the marine environment fundamentally influences the growth and physiology of microorganisms. Organic and inorganic chemicals that are produced by some marine plankton can be consumed by other plankton for energy production, growth, or to initiate essential physiological processes. Cultures of the diatom Thalassiosira pseudonana,...
    Resource Type:
    Dissertation
    Full Text:
    life cycle stages of T. pseudonana (A-K), T. weissflogii (L) and C. cryptica (M- 126 O) imaged using

  • Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

    Creator:
    Bertagnolli, Anthony D.
    Abstract:
    Continental shelves located along eastern boundary currents occupy relatively small volumes of the world’s oceans, yet are responsible for a large proportion of global primary production. The Oregon coast is among these ecosystems. Recent analyses of dissolved oxygen at shallow depths in the water column has suggested increasing episodes of...
    Resource Type:
    Dissertation
    Full Text:
    region of the water column to become hypoxic when oxygen declines. Surface (5 m) and BBL (1-10 m above

  • Disulphide bond formation is essential for vaccinia virus late protein L1R function and for production of infectious progeny

    Creator:
    Blouch, Robert Edward
    Abstract:
    L1R, a myristylated late gene product of vaccinia virus, is essential for formation of infectious intracellular mature virions (IMV). In its absence, only viral particles arrested at an immature stage are detected and no infectious progeny virus are produced. Previous studies have shown that the L1R protein is exclusively associated...
    Resource Type:
    Masters Thesis
    Full Text:
    throughout the years I have spent in his lab. Secondly, to Chelsea M. Byrd and Brita Hanson whose work with

  • Analysis of Bacteroidales 16S rRNA gene sequences from a geographically isolated human population

    Creator:
    Petersen, Carolyn A.
    Abstract:
    Standard methods of measuring fecal pollution in water do not distinguish between human and non-human sources. Molecular technology enabled the development of host-specific markers that distinguish fecal sources. Human specific PCR primers, HF183F and HF134F, were designed based on phylogenetic analyses of partial 16S rRNA gene sequences from the Bacteroidales...
    Resource Type:
    Masters Thesis
    Full Text:
    , and 3 washes were done with SEWS-M buffer. Water samples: Bacterial DNA from water samples was

  • Structure-function analysis of the vaccinia virus I7L proteinase

    Creator:
    Moerdyk, Megan J.
    Abstract:
    Vaccinia virus is the prototypic member of the Orthopoxvirus genus. It undergoes a complex replication process where a key step in the transition from immature virion to intracellular mature virion is the cleavage of the major core protein precursors. The product of the I7L open reading frame (ORF) is a...
    Resource Type:
    Masters Thesis
    Full Text:
    and information received from employees at SIGA Technologies, Inc. In particular, Dr. Chelsea M