A number of bacterial species are capable of degrading the widespread environmental pollutant trichloroethylene (TCE) via aerobic cometabolism, but cytotoxic effects that can debilitate the microorganism often accompany this transformation. In this dissertation the effects of TCE degradation on the well-studied, toluene-oxidizing bacterium Burkholderia cepacia G4 were investigated at the...
Butane-grown Pseudomonas butanovora oxidized butane by a soluble butane monooxygenase through the terminal pathway yielding 1 -butanol as the predominant product. Alcohol dehydrogenases (ADHs) involved in butane oxidation in P. butanovora were purified and characterized at the biochemical, genetic and physiological levels. Butane-grown P. butanovora expressed a type I soluble...
Butane-grown cells of Pseudomonas butanovora, Nocardioides sp. CF8 and Mycobacterium vaccae JOB5 were tested for their ability to cooxidize methane, ammonia and ethylene. Less than 10 nmol of methane were degraded by each of the bacteria (0.17-0.35 mg protein) in 30 minutes. Hydroxylamine and nitrite accumulated when Nocardioides CF8 and...
Iron is absolutely necessary for the maintenance, the defense, the differentiation and
last, but by no means least, the growth and cellular division of almost all living
organisms including Nitrosomonas europaea, an ammonia oxidizing bacterium. Given
the necessity of iron, it is not surprising that N. europaea evolved several irondependent...