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Filtering by: Creator Rohrer, M. Advisor Barnes, David W.

Studies on the growth inhibition and differentiation of serum-free mouse embryo (SFME) cells

Creator:

Varga Weisz, Patrick D.

Abstract:

Serum-free mouse embryo (SFME) cells are derived in medium in which serum is replaced with growth factors and other supplements. They display unusual properties. They do not lose proliferative potential or show gross chromosomal aberration upon extended culture, they depend on epidermal growth factor (EGF) for survival, and are reversibly...

Resource Type:

Dissertation

Full Text:

and was not dialyzable against 1 M acetic acid. The activity could be precipitated by methanol
Nonsenecent serum-free mouse proastroblasts : extended culture, growth responses in vitro, and application to the culture of human embryonic astrocytes

Creator:

Loo, Deryk Thomas

Abstract:

Mouse embryo cells cultured in vitro in serum-supplemented media undergo growth crisis, resulting in the loss of genomically normal cells prior to the appearance of established, aneuploid cell lines. I used the technique of serum-free cell culture to develop a serum-free mouse embryo (SFME) cell line in which serum was...

Resource Type:

Dissertation