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Creator:: Quinn, Richard Paul
Abstract:: The thermophilic aldolase from Bacillus stearothermophilus (NCA 2184) has been purified to a specific activity of 45.5 μ-moles of fructose-1, 6-diphosphate cleaved per minute per mg of enzyme, representing a 624-fold increase over the crude extract. The preparation appeared to be homogenous by sedimentation velocity ultra-centrifugation and by a specific...
Resource Type:: Dissertation
Full Text:: Biophysics Redacted for privacy Dean of Graduate School' Date thesis is presented attl) gyp, q" Typed by J

Creator:: Miller, Terry L.
Abstract:: The spectrophotometric titration of the tyrosine residues, their reaction with N-acetylimidazole, and the effect of pH on the thermally induced conformational transition of ribonuclease T₁ have been investigated. The results of spectrophotometric titration in 0.20 M KCl/0.001 M sodium phosphate suggest that titration of the tyrosine residues occurs in two...
Resource Type:: Dissertation
Full Text:: mµ has been observed in the spectrophotometric titration of acid-denatured RNase A (Bigelow, 1961

Creator:: McParland, Reginald Harry
Abstract:: The halophilic alkaline phosphatase of Halobacterium salinarium has been purified to a specific activity of 3,000-3,200 units per mg of protein. The purification scheme consisted of acetone and ammonium sulfate fractionations, and molecular sieving and adsorption chromatography on Sephadex G -50. The enzyme was stabilized during purification by the presence...
Resource Type:: Dissertation
Full Text:: (EDTA), and trichloroacetic acid (TCA) were from J. T. Baker Chemical Company, Phillipsburg, New

Creator:: Chung, Po-luen
Abstract:: A comparative study was made on the effect of urea denaturation of bovine serum albumin (BSA), polyglycyl BSA and poly-L-phenylalanyl BSA. The denaturation process was followed by ultraviolet spectrophotometry and optical rotation. A blue shift in the absorption maximum at 278 mμ was observed in urea denatured proteins, In 7M...
Resource Type:: Masters Thesis
Full Text:: purchased from J. T. Baker Chemical Co. , 2. 16 ml of 25% solution in methanol and dilute to 100 ml. The

Creator:: Harris, Ward Edwin
Abstract:: A heat stable derivative of bovine serum albumin containing 300 residues of glycine added to 30 sites on the surface of the protein has been prepared. This derivative may be heated to 100° C for prolonged periods of time without aggregation. By comparison, native BSA aggregates at 62° C under...
Resource Type:: Dissertation
Full Text:: acid. Bigelow (9) has taken the values of the hydrophobic nature of the various amino acids as

Creator:: Tiffany, Thomas Oliver
Resource Type:: Dissertation
Full Text:: from Hellige Inc. , Garden City, New York. Undecane and dodecane were purchased from the J. T. Baker

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