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Filtering by: Creator Williams, Reginald Advisor Becker, Robert R.

Purification and characterization of an alkaline phosphatase from Halobacterium salinarium

Creator:

McParland, Reginald Harry

Abstract:

The halophilic alkaline phosphatase of Halobacterium salinarium has been purified to a specific activity of 3,000-3,200 units per mg of protein. The purification scheme consisted of acetone and ammonium sulfate fractionations, and molecular sieving and adsorption chromatography on Sephadex G -50. The enzyme was stabilized during purification by the presence...

Resource Type:

Dissertation

Full Text:

AN ABSTRACT OF THE THESIS OF REGINALD HARRY MCPARLAND (Name