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Filtering by: Creator Olsen, Keith A. Advisor Brunner, Amy M. File Format application/pdf

Identifying candidate genes associated with cold adaptation in Douglas-fir using DNA microarrays

Creator:

Howe, Dana Kathryn

Abstract:

In forest trees from temperate and boreal regions, cold acclimation is an important adaptive trait that involves changes in gene expression and physiology. Genecological, quantitative genetic, and QTL studies have been used to study the genetics of cold acclimation in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), but the genes responsible for...

Resource Type:

Masters Thesis

Full Text:

remain unknown. Douglas-fir seedlings from a low elevation, coastal Oregon population and a high
Stability of reporter gene expression and RNAi in transgenic poplars over multiple years in the field under vegetative propagation

Creator:

Li, Jingyi

Abstract:

Stable expression of transgenes is required for commercial uses of genetically engineered trees. To better understand the stability of transgene expression under field conditions, we studied transgene expression and RNA interference (RNAi)-induced transgene suppression in 2,480 transgenic poplars (460 transgene insertion events) over three years. Stability of expression was assessed...

Resource Type:

Dissertation

Full Text:

based on two reporter genes, green fluorescent protein (GFP) driven by a 35S promoter and the