Data from nuclear ribosomal internal transcribed spacer regions (nrITS) and chloroplast DNA (cpDNA) have failed to resolve phylogenetic relationships in Pinus. To provide greater interspecific resolution, five low-copy nuclear genes were developed from mapped conifer anchor loci. Four genes were sequenced from species representing all Pinus subsections. Individual loci do...
A fossil-calibrated phylogenetic framework based on exemplars from each of the four taxonomic sections within Pinus was created using multiple nuclear and chloroplast loci. Calibration at the well-defined subgeneric split within Pinus with either fossil leaves and cones (ca. 45 million years ago) or fossil wood (ca. 85 million years...