For the past twenty-seven years, Human Immunodeficiency Virus (HIV)/Acquired Immunodeficiency Syndrome (AIDS) has become a worldwide pandemic. Due to the properties of HIV, such as its fast replication rate and error-prone reverse transcriptase, researchers have been unsuccessful in finding a cure or vaccine. Researchers have, however, developed a treatment regimen...
In this thesis, I studied the translation of dengue virus RNA using a luciferase reporter gene system in Vero cells. The dengue reporter mRNA construct, which harbors 5´-terminal viral nts and 3´-terminal nts, could be translated efficiently compared to an alpha globin reporter construct. The 5´-cap structure and 3´-untranslated region...