PART I. The concentration of nickel in some soils may be
insufficient to meet the requirements of enzymes such as
urease in soybeans and hydrogenase in Rhizobium. Nickel
availability in soils was examined by measuring microbial
urease activity in soils which were treated with glucose
and urea. Supplemental nickel was...
An investigation was undertaken to determine whether
potassium is indeed necessary for the in vivo synthesis of a
specific protein. The effects of univalent cations on the
inductive formation of nitrate reductase and on the level of
activity of representative non-adaptive enzyme are presented.
Cultures of Neurospora crassa were grown...
The primary purpose of this investigation was to determine the
conditions necessary for the preparation of cell-free extracts of
nodule bacteroids capable of catalysing the reduction of acetylene to
ethylene and nitrogen to ammonia. Initial investigations were conducted
to determine whether intact soybean root nodules would reduce
acetylene to ethylene...
Alnus rubra (Bong. ) seedlings were grown in sand culture and
irrigated with nutrient solution containing CdCl₂
ranging from 5 μg to 100 mg per liter. Treatment of A. rubra seedlings for 4 weeks
with 50 and 100 mg CdCl₂
per liter of nitrogen-free nutrient solution
decreased in situ nitrogenase...
A major objective of this investigation was to determine the
possible roles, if any, of poly-β-hydroxybutyrate and products of
its metabolic breakdown in symbiotic nitrogen fixation by nodule
bacteroids. Changes in poly -β- hydroxybutyrate content and in
activities of nitrogenase, β-hydroxybutyrate dehydrogenase, and
isocitrate lyase were studied under various conditions...
H₂-uptake positive strains (122 DES and SR) and H₂-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H₂-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of ¹⁴CO₂ uptake by hydrogenase-induced cells was about six times greater in...
Ribonucleotide reductase from Rhizobium meliloti has been
partially purified and characterized. The enzymatic reduction of
ribonucleotides to deoxyribonucleotides is dependent upon B₁₂ coenzyme
and dihydrolipoate.
B₁₂ coenzyme was more effective than B₁₂ coenzyme analogues
in the reductase reaction. The affinity of the R. meliloti
reductase system for B₁₂ coenzyme was...