Ribonucleotide reductase from Rhizobium meliloti has been
partially purified and characterized. The enzymatic reduction of
ribonucleotides to deoxyribonucleotides is dependent upon B₁₂ coenzyme
and dihydrolipoate.
B₁₂ coenzyme was more effective than B₁₂ coenzyme analogues
in the reductase reaction. The affinity of the R. meliloti
reductase system for B₁₂ coenzyme was...
A major objective of this investigation was to determine the
possible roles, if any, of poly-β-hydroxybutyrate and products of
its metabolic breakdown in symbiotic nitrogen fixation by nodule
bacteroids. Changes in poly -β- hydroxybutyrate content and in
activities of nitrogenase, β-hydroxybutyrate dehydrogenase, and
isocitrate lyase were studied under various conditions...
H₂-uptake positive strains (122 DES and SR) and H₂-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H₂-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of ¹⁴CO₂ uptake by hydrogenase-induced cells was about six times greater in...
The primary purpose of this investigation was to determine the
conditions necessary for the preparation of cell-free extracts of
nodule bacteroids capable of catalysing the reduction of acetylene to
ethylene and nitrogen to ammonia. Initial investigations were conducted
to determine whether intact soybean root nodules would reduce
acetylene to ethylene...
Glutamine synthetase activity is present in both the cytosol and
bacteroid fractions of soybean root nodules. Total activity measurements
indicate that at least 90% of the activity in nodules is located
in the cytosol. Results reported by McParland, et al. (29), showed
that many properties of glutamine synthetase from nodule...
Experiments are reported which demonstrate that a cobalt deficiency
in R. meliloti results in a decreased cytochrome content of
bacterial cells. It is concluded that the effect of cobalt deficiency
on cytochrome content of Rhizobium cells and on the leghemoglobin
content of nodules possibly may be explained by an effect...