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Hydrogen-stimulated CO₂ fixation and coordinate derepression of hydrogenase and ribulosebisphosphate carboxylase in a H₂-uptake positive strain of Rhizobium japonicum

Creator:: Simpson, Frank Berry
Abstract:: H₂-uptake positive strains (122 DES and SR) and H₂-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H₂-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of ¹⁴CO₂ uptake by hydrogenase-induced cells was about six times greater in...
Resource Type:: Masters Thesis
Full Text:: a H 2 -Uptake Positive Strain of Rhizobium japonicum by Frank Berry Simpson A THESIS submitted

Some properties of ribonucleotide reductase in Rhizobium species

Creator:: Cowles, Joe Richard, 1941-
Abstract:: Ribonucleotide reductase from Rhizobium meliloti has been partially purified and characterized. The enzymatic reduction of ribonucleotides to deoxyribonucleotides is dependent upon B₁₂ coenzyme and dihydrolipoate. B₁₂ coenzyme was more effective than B₁₂ coenzyme analogues in the reductase reaction. The affinity of the R. meliloti reductase system for B₁₂ coenzyme was...
Resource Type:: Dissertation
Full Text:: REDUCTASE IN RHIZOBIUM SPECIES Abstract approved: Redacted for privacy Harold J. Evans Ribonucleotide

Propionate metabolism and its relationship to leghemoglobin biosynthesis in soybean nodules

Creator:: Jackson, Earl Kenneth
Abstract:: Experiments are reported which demonstrate that a cobalt deficiency in R. meliloti results in a decreased cytochrome content of bacterial cells. It is concluded that the effect of cobalt deficiency on cytochrome content of Rhizobium cells and on the leghemoglobin content of nodules possibly may be explained by an effect...
Resource Type:: Dissertation
Full Text:: . Harold J. Evans for suggesting the problem, for helpful advice, and for constructive criticism during

Measurement of nitrogenase activity of intact legume symbionts in situ using the acetylene reduction assay

Creator:: Fishbeck, Kathleen Ann
Abstract:: The process of biological nitrogen fixation involves the enzymic reduction of atmospheric nitrogen (N₂) to ammonia (NH₃). The enzyme which catalyzes this reduction, nitrogenase, does not exhibit a high degree of substrate specificity. It also catalyzes the reduction of acetylene (C₂2H₂) to ethylene (C₂H₄). The reduction of C₂H₂ to C₂H₄...
Resource Type:: Masters Thesis
Full Text:: ACKNOWLEDGEMENTS I would like to express sincere appreciation to Dr. Harold J. Evans for his guidance and

Properties of the hydrogenase system in bacteroids of soybean root nodules

Creator:: McCrae, Roberta Ellen
Resource Type:: Masters Thesis
Full Text:: pM and an apparent KM for 0 2 of 1.3 pM. A linear relationship exists between the rate of H 2

Purification and properties of a ferredoxin from Rhizobium japonicum bacteroids

Creator:: Carter, Kevin Richard
Resource Type:: Dissertation
Full Text:: 1900 nmol C 2 H 4 produced.min -1 -mg 1 in the reaction mediating electron transfer between

Purification and some properties of nitrogenase from soybean root nodules

Creator:: Israel, Daniel Wesley
Resource Type:: Dissertation
Full Text:: Privacy V 0----Harold J. Evans The Mo-Fe protein from bacteroids of soybean nodules has been purified

A biochemical investigation of the nitrogenase system from soybean root nodules

Creator:: Koch, Burton Lee
Abstract:: The primary purpose of this investigation was to determine the conditions necessary for the preparation of cell-free extracts of nodule bacteroids capable of catalysing the reduction of acetylene to ethylene and nitrogen to ammonia. Initial investigations were conducted to determine whether intact soybean root nodules would reduce acetylene to ethylene...
Resource Type:: Dissertation
Full Text:: : Harold J. Evans The primary purpose of this investigation was to determine the conditions necessary for

Investigations into the physiology of nitrogen fixation : Part I. Nickel metabolism of plants and bacteria : Part II. Peroxide scavenging in soybean root nodules

Creator:: Dalton, David Andrews
Abstract:: PART I. The concentration of nickel in some soils may be insufficient to meet the requirements of enzymes such as urease in soybeans and hydrogenase in Rhizobium. Nickel availability in soils was examined by measuring microbial urease activity in soils which were treated with glucose and urea. Supplemental nickel was...
Resource Type:: Dissertation
Full Text:: Nodules Abstract approved: v (Harold J. Evans) Redacted for privacy PART I. The concentration of

Enzymatic and respiratory studies on the role of cobalt in legumes

Creator:: De Hertogh, August
Resource Type:: Dissertation
Full Text:: his sincere appreciation to Dr. Harold J. Evans for suggesting the problem, and for his interest

ScholarsArchive@OSU

Hydrogen-stimulated CO₂ fixation and coordinate derepression of hydrogenase and ribulosebisphosphate carboxylase in a H₂-uptake positive strain of Rhizobium japonicum

Some properties of ribonucleotide reductase in Rhizobium species

Propionate metabolism and its relationship to leghemoglobin biosynthesis in soybean nodules

Measurement of nitrogenase activity of intact legume symbionts in situ using the acetylene reduction assay

Properties of the hydrogenase system in bacteroids of soybean root nodules

Purification and properties of a ferredoxin from Rhizobium japonicum bacteroids

Purification and some properties of nitrogenase from soybean root nodules

A biochemical investigation of the nitrogenase system from soybean root nodules

Investigations into the physiology of nitrogen fixation : Part I. Nickel metabolism of plants and bacteria : Part II. Peroxide scavenging in soybean root nodules

Enzymatic and respiratory studies on the role of cobalt in legumes

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