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Filtering by: Creator Maki, Arthur Advisor Forsberg, Neil E.

Purification and characterization of recombinant calpain-5

Creator:

Wang, Mei-Chuan

Abstract:

Recombinant human calpain-5 was expressed in insect cells using a baculovirus system. The expressed calpain-5 was purified by both traditional chromatography and by affinity-column chromatography. Both methods yielded active protease. Calpain-5 displayed very limited hydrophobicity. This indicated that calpain-5 is not a membrane binding protein. Calpain-5 had pI of 8.3....

Resource Type:

Dissertation
Regulation of skeletal muscle protein degradation by u-calpain and development of a skeletal muscle-specific inducible expression system

Creator:

Xiao, Ying-Yi

Abstract:

The first goal of this study was to understand the role of u-calpain in skeletal muscle protein degradation in cultured muscle cells. Several strategies were developed to down-regulate endogenous u-calpain activity and m-calpain activity in rat myotubes. These included over-expression of antisense u-calpain (AnsL), dominant negative u-calpain (DN-u-CL), antisense 30K...

Resource Type:

Dissertation

Full Text:

:625-22. 19. Arthur, J.S.C., Elce, J.S., Hegadorn, C., Williams, K., and Greer, P.A. (2000) Disruption