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Oral immunization for the control of vibriosis in Pacific salmon

Creator:: Nelson, Jim Steel
Abstract:: An oral vaccine was developed for control of vibriosis in Pacific salmon. The vaccine consists of a lyophilized sonicate of cells of Vibrio anguillarum, the causative agent of this disease. The preparation was incorporated into a ration and fed to salmon in fresh water before the fish were transported and...
Resource Type:: Masters Thesis
Full Text:: sharply increased to 58% whereas 200 and 300 p,g resulted in 33% mortality. Salmon receiving no vaccine

Development of a new vaccine delivery system for immunizing fish and investigation of the protective antigens in Vibrio anguillarum

Creator:: Gould, Rowan Wayne
Abstract:: Phenol-water and trichloroacetic acid extracts of Vibrio anguillarum were both antigenic and immunogenic when injected into coho salmon (Oncorhynchus kisutch). Cross-protection against virulent Vibrio anguillarum was observed with whole-cell bacterins and phenol-water extracts prepared from two serologically distinct groups. Gel diffusion studies indicated that intact somatic antigens were serotype specific....
Resource Type:: Dissertation
Full Text:: spray apparatus operated at 6.3 to 7. 0 kilograms per square centi- meter (90 to 100 pounds per square

Yersinia ruckeri : a characterization of the carrier state in steelhead trout (Salmo gairdneri)

Creator:: Hunter, Valerie Antoinette
Abstract:: Fish, asymptomatically infected with Enteric Redmouth Bacterium (ERMB), were tested for their ability to transmit bacteria to non-infected fish. Unstressed carrier fish were unable to transmit enough bacteria to cause epizootics or produce carrier fish. However, when the ERMB carriers were stressed with heat, sufficient bacteria were shed to infect...
Resource Type:: Masters Thesis
Full Text:: period, no bacteria was detectable and at no time were these fish capable of infecting other healthy

Pathogenic, antigenic, and immunogenic properties of Aeromonas salmonicida studied in juvenile coho salmon (Oncorhynchus kisutch)

Creator:: Udey, Lanny R.
Abstract:: An additional layer, exterior to the outer membrane cellwall layer, was found on Aeromonas salmonicida cells from aggregating strains. Nonaggregating strains were found to lack this layer. The nonaggregating strains were all avirulent (LD₅₀ greater than 1 X 10 ⁸ CFU/fish) while the majority of the aggregating strains were virulent...
Resource Type:: Dissertation
Full Text:: (no longer manufactured) prior to injection. Serum was collected 90 to 95 days after injection. Fish

Enteric Redmouth Bacterium of salmonids : a biochemical and serological comparison of selected isolates

Creator:: O'Leary, Patrick John
Abstract:: A comparison of the agglutinating and precipatiating antigens of Enteric Redmouth Bacterium (ERMB) was made. There are two major and one minor agglutinating antigens which describe two serotypes (I and II). Only serotype I metabolizes sorbitol. A bacterin from serotype I cross protected against a challenge of bacteria of serotype...
Resource Type:: Masters Thesis
Full Text:: agar are cream colored, smooth, circular and slightly raised with entire edges. No pigmentation has

Certain chemical and immunological properties of the endotoxin from Vibrio anguillarum

Creator:: Abe, Patrick Masami
Abstract:: The Boivin agent from Vibrio anguillarum was extracted with the use of 0.25M trichloroacetic acid (TCA). Chemical analysis revealed that the endotoxin contained approximately 35.1% protein, 18.1% carbohydrate, 39.3% lipid, 1.8% glucosamine and evidence of ethanolamine. Sixteen different amino acids made up the protein. The aliphatic amino acid concentration was...
Resource Type:: Masters Thesis
Full Text:: obtained by injection than by feeding of the endotoxin. No anti-V. anguillarum agglutinating antibodies

Partial characterization of six established salmonid cell lines

Creator:: Philippon-Fried, Marie
Abstract:: Six salmonid cell lines previously established in this laboratory, CHSE 114 and CHSE 214 (chinook salmon), CSE 119 (coho salmon), SSE 5 (sockeye salmon), STE 137 (steelhead trout) and RTH 149 (rainbow trout), were partially characterized. Mycoplasmas were not detected in any of the cell lines. All cell lines exhibited...
Resource Type:: Masters Thesis
Full Text:: number for this cell line was no longer changing. Saturation density and optimum growth cell density

Isolation and characterization of a leukocytolytic factor from Aeromonas salmonicida

Creator:: Fuller, David Wallace
Abstract:: The purpose of this study was to find what effect temperature and immunization has on the phagocytic activity of fish leukocytes against certain disease causing bacteria. Also proposed was the isolation and characterization of a leukocytolytic factor produced by one of these bacteria, Aeromonas salmonicida. Investigations revealed that temperature had...
Resource Type:: Dissertation
Full Text:: produced by one of these bacteria, Aeromonas salmonicida. Investigations revealed that temperature had no

Properties of anti- Aeromonas salmonicida antibodies from juvenile coho salmon (Oncorhynchus kisutch)

Creator:: Cisar, John Ormond
Abstract:: After parenteral immunization with Aeromonas salmonicida cells, the agglutination activity in juvenile coho salmon (Oncorhynchus kisutch) serum was associated with the macroglobulin serum fraction. Antibody activity was diminished by 30 minutes exposure to 50 C but not 45 C. Reduction with 2-mercaptoethanol and alkylation markedly lowered but did not completely...
Resource Type:: Dissertation
Full Text:: . salmonicida rnacroglobulin was composed of four IgM-like subunits. No evidence for a low molecular weight

Bactericidal and physico-chemical properties of a lectin derived from spring chinook salmon (Oncorhynchus tshawytscha) Ova

Creator:: Banowetz, Gary Michael
Abstract:: Unfertilized ova from spring chinook salmon (Oncorhynchus tshawytscha) were examined for the presence of classical salmonid inmmunoglobulin. Immunodiffusion techniques in which rabbit anti-salmonid immunoglobulin was reacted against an ova homogenate and rabbit anti-ova homogenate was reacted against immune chinook serum failed to detect classical-type antibodies within these eggs. A lectin...
Resource Type:: Dissertation
Full Text:: % of the bacteria were no longer motile. No cell lysis was observed. The bacterial inhibition

ScholarsArchive@OSU

Oral immunization for the control of vibriosis in Pacific salmon

Development of a new vaccine delivery system for immunizing fish and investigation of the protective antigens in Vibrio anguillarum

Yersinia ruckeri : a characterization of the carrier state in steelhead trout (Salmo gairdneri)

Pathogenic, antigenic, and immunogenic properties of Aeromonas salmonicida studied in juvenile coho salmon (Oncorhynchus kisutch)

Enteric Redmouth Bacterium of salmonids : a biochemical and serological comparison of selected isolates

Certain chemical and immunological properties of the endotoxin from Vibrio anguillarum

Partial characterization of six established salmonid cell lines

Isolation and characterization of a leukocytolytic factor from Aeromonas salmonicida

Properties of anti- Aeromonas salmonicida antibodies from juvenile coho salmon (Oncorhynchus kisutch)

Bactericidal and physico-chemical properties of a lectin derived from spring chinook salmon (Oncorhynchus tshawytscha) Ova

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