The occurrence and distribution of fish viruses in Oregon were
determined by the examination of anadromous and fresh water salmonids.
Infectious pancreatic necrosis virus (IPNV) was isolated and
identified from asymptomatic adult coho (Oncorhynchus kisutch) and
chinook (0. tshawytscha) salmon returning to the Columbia River
system. Infectious pancreatic necrosis virus...
This research was initiated to investigate the effects of various
parameters on the immune response of juvenile coho salmon to Aeromonas
salmonicida. The antibody response and development of protective
immunity in juvenile coho salmon were followed after a single
intraperitoneal injection of formalin killed A. salmonicida cells emulsified
in Freund's...
After parenteral immunization with Aeromonas salmonicida
cells, the agglutination activity in juvenile coho salmon (Oncorhynchus
kisutch) serum was associated with the macroglobulin serum fraction.
Antibody activity was diminished by 30 minutes exposure to 50 C
but not 45 C. Reduction with 2-mercaptoethanol and alkylation
markedly lowered but did not completely...
An additional layer, exterior to the outer membrane cellwall
layer, was found on Aeromonas salmonicida cells from aggregating
strains. Nonaggregating strains were found to lack this layer.
The nonaggregating strains were all avirulent (LD₅₀
greater than
1 X 10
⁸ CFU/fish) while the majority of the aggregating strains
were virulent...
Six salmonid cell lines previously established in this laboratory,
CHSE 114 and CHSE 214 (chinook salmon), CSE 119 (coho salmon),
SSE 5 (sockeye salmon), STE 137 (steelhead trout) and RTH 149 (rainbow
trout), were partially characterized. Mycoplasmas were not
detected in any of the cell lines.
All cell lines exhibited...
An oral vaccine was developed for control of vibriosis
in Pacific salmon. The vaccine consists of a lyophilized
sonicate of cells of Vibrio anguillarum, the causative
agent of this disease. The preparation was incorporated
into a ration and fed to salmon in fresh water before the
fish were transported and...
Efficacious vaccines were developed for the control of Vibrio
anguillarum, the etiological agent of vibriosis in salmonid fish.
These bacterins can be administered either orally or parenterally.
It was determined that both formalin-killed lyophilized whole cells
and wet-packed whole cells of the organism are effective oral immunogens.
Intraperitoneal injection of...
A comparison of the agglutinating and precipatiating
antigens of Enteric Redmouth Bacterium (ERMB) was made.
There are two major and one minor agglutinating antigens
which describe two serotypes (I and II). Only serotype I
metabolizes sorbitol. A bacterin from serotype I cross
protected against a challenge of bacteria of serotype...
The distribution in Oregon of two viruses affecting salmonid
fishes was determined by examination of hatchery and feral fish.
Infectious pancreatic necrosis virus (IPNV) was isolated from fish
at the Oregon Department of Fish and Wildlife Research Laboratory,
Corvallis, Roaring River, Fort Klamath, and Wizard Falls Hatcheries.
Fish collected at...