Index Catalog // ScholarsArchive@OSU

Single-Cell Genomics of Uncultivated Marine Bacteria

Creator:: Landry, Zachary
Abstract:: Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
Resource Type:: Dissertation
Full Text:: first true single-cell, full-genome analysis from the marine environment, revealing the genome of a

Marine bacterioplankton abundances and distributions

Creator:: Morris, Robert M.
Abstract:: Microorganisms play key roles in ocean biogeochemistry. However, several predominant groups of uncultured bacterioplankton thought to contribute to important biogeochemical processes in the oceans are known primarily from gene cloning studies. Although these studies have greatly expanded our view of microbial diversity in the oceans, they are not quantitative and...
Resource Type:: Dissertation
Full Text:: microbes in oceanographic and biogeochemical processes. In this study, direct cell counts Redacted for

Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

Creator:: Carini, Paul J.
Abstract:: Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
Resource Type:: Dissertation
Full Text:: that Ca. P. ubique has an apparent requirement for 10.2 attomoles Pi cell-1 and Pelagibacter sp. str

Culturing uncultured environmental microorganisms

Creator:: Connon, Stephanie A.
Abstract:: Research on natural environments, over the last decade, is replete with microbial diversity studies that used culture-independent approaches. The cloning and sequencing of the 16S rRNA genes has been the driving force in the expansion of awareness about the great diversity of previously undiscovered microorganisms. Well-known uncultured groups of microorganisms...
Resource Type:: Dissertation
Full Text:: culture collection. It is no longer assumed that cultures acquired from an environment represent the

Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

Creator:: Bertagnolli, Anthony D.
Abstract:: Continental shelves located along eastern boundary currents occupy relatively small volumes of the world’s oceans, yet are responsible for a large proportion of global primary production. The Oregon coast is among these ecosystems. Recent analyses of dissolved oxygen at shallow depths in the water column has suggested increasing episodes of...
Resource Type:: Dissertation
Full Text:: high cell densities have made it an invaluable tool for investigating the biochemical and molecular

Characterization of novel marine oligotrophic bacteria isolated from the Pacific Ocean : description of Marinivirgula fluito gen. nov., sp. nov., Marinivirgula obesa gen. nov., sp. nov. and Litincola parvulus gen. nov., sp. nov.

Creator:: Shin, Eun Jung
Abstract:: A new high-throughput culturing (HTC) method using a low nutrient heterotrophic medium (LNHM) has led to the isolation of many novel strains of oligotrophic bacteria from marine ecosystems. Four strains belonging to a single dade, HTCC2151, HTCC218OT, HTCC2178T and HTCC2188T, were isolated from the coast of Oregon by the HTC...
Resource Type:: Masters Thesis
Full Text:: Gammaproteobacteria.................. 42 LIST OF TABLES Table 1. Cell morphology, size, and biovolume of the HTCC

An analysis of Sargasso Sea bacterioplankton diversity using 16S ribosomal RNA

Creator:: Britschgi, Theresa Baden
Abstract:: The objective of this project was to use ribosomal RNA genes, cloned from natural populations of Sargasso Sea bacterioplankton, as markers for picoplankton diversity. It is widely recognised that a majority of microorganisms have yet to be cultivated, and therefore much of extant microbial diversity remains unknown (50). The method...
Resource Type:: Masters Thesis
Full Text:: on YT agar slants containing 75 mg /ml kanamycin (Sigma). If1 origin Amp Pst I pBLUESCRIPT KSII

The isolation of Lactococcus lactis subsp. cremoris from nature with probes for 16S ribosomal RNAs

Creator:: Salama, Maysoon
Resource Type:: Dissertation
Full Text:: . lactis subsp. cremoris. Thirty-eight plant and vegetable species, twelve other samples from Redacted

Disturbances in a marine bacterioplankton system drive community assembly towards niche-based processes

Creator:: Vergin, Kevin L.
Abstract:: Over 100 monthly bacterioplankton DNA samples, from each of the surface and 200 m depths at the Bermuda Atlantic Time-series Study (BATS) site, were analyzed for community assembly processes. Correlation networks, filtered for potential autocorrelation artifacts, were constructed for each depth. Network characteristics for the two depths were remarkably similar...
Resource Type:: Masters Thesis
Full Text:: make gene sequence abundance difficult to translate to cell numbers in the environment. Secondly

ScholarsArchive@OSU

Single-Cell Genomics of Uncultivated Marine Bacteria

Marine bacterioplankton abundances and distributions

Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

Culturing uncultured environmental microorganisms

Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

Characterization of novel marine oligotrophic bacteria isolated from the Pacific Ocean : description of Marinivirgula fluito gen. nov., sp. nov., Marinivirgula obesa gen. nov., sp. nov. and Litincola parvulus gen. nov., sp. nov.

An analysis of Sargasso Sea bacterioplankton diversity using 16S ribosomal RNA

The isolation of Lactococcus lactis subsp. cremoris from nature with probes for 16S ribosomal RNAs

Disturbances in a marine bacterioplankton system drive community assembly towards niche-based processes

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