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Filtering by: Creator Zimmer, C. Advisor Giovannoni, Stephen J. Degree Name Doctor of Philosophy (Ph.D.)

Proteomic analyses of marine bacteria

Creator:

Sowell, Sarah M.

Abstract:

Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...

Resource Type:

Dissertation

Full Text:

Accurate Mass and Time (AMT) tag library………………………………………………… 163 C. Relative quantification of Cand
The proteomic and transcriptomic responses to iron, sulfur, and nitrogen limitation in the abundant marine bacterium Candidatus Pelagibacter ubique

Creator:

Smith, Daniel P.

Abstract:

Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...

Resource Type:

Dissertation

Full Text:

base pairs, which require one less atom of nitrogen compared to a G-C base pair [102]. Though Ca. P
Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

Creator:

Carini, Paul J.

Abstract:

Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...

Resource Type:

Dissertation

Full Text:

the raw materials (for example, core nutrients such as N, P and C) (25, 39, 40). Morris has put