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Filtering by: Creator Keller, C. Remove constraint Creator: Keller, C. Advisor Giovannoni, Stephen J. Remove constraint Advisor: Giovannoni, Stephen J. License All rights reserved Remove constraint License: All rights reserved

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  • Outliers: The Unusual Characteristics of Marine Carbon-Oxidizing Bacterial Cells Adapted to Oligotrophic Conditions

    Creator:
    Noell, Stephen E.
    Abstract:
    Oligotrophic bacteria are adapted to low nutrient concentrations. As most of the ocean is oligotrophic (low in nutrients), oligotrophic bacteria thrive in the ocean and make up the majority of planktonic cells. Because of this, oligotrophs are crucial to oceanic nutrient cycles. However, marine oligotrophic bacteria are dwarfed by their...
    Resource Type:
    Dissertation
    Full Text:
    Uptake and oxidation of 14C-L-alanine (14C-LALA) in (A, C, E) an oligotroph, Ca. P. st. HTCC7211, and (B

  • Single-Cell Genomics of Uncultivated Marine Bacteria

    Creator:
    Landry, Zachary
    Abstract:
    Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
    Resource Type:
    Dissertation

  • Metabolism of one carbon (C1) and methylated compounds in SAR11 bacteria

    Creator:
    Sun, Jing
    Abstract:
    SAR11 Alphaproteobacteria are the most abundant aerobic chemoheterotrophs in ocean surface waters. Previous studies have indicated SAR11 cells play an important role in marine carbon cycling and consume up to half of some common dissolved organic compounds, such as amino acids. During sequencing of the first SAR11 genome, genes for...
    Resource Type:
    Dissertation
    Full Text:
    the bioinformatics analyses. Dr. Kimberly H. Halsey, Amy E. Carter, Zachary C. Landry and Dr

  • Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

    Creator:
    Carini, Paul J.
    Abstract:
    Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
    Resource Type:
    Dissertation
    Full Text:
    the raw materials (for example, core nutrients such as N, P and C) (25, 39, 40). Morris has put

  • Culturing uncultured environmental microorganisms

    Creator:
    Connon, Stephanie A.
    Abstract:
    Research on natural environments, over the last decade, is replete with microbial diversity studies that used culture-independent approaches. The cloning and sequencing of the 16S rRNA genes has been the driving force in the expansion of awareness about the great diversity of previously undiscovered microorganisms. Well-known uncultured groups of microorganisms...
    Resource Type:
    Dissertation
    Full Text:
    site and stored at 4°C for up to one week prior to staining with 5 jig/mi DAPI and filtering down onto

  • Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

    Creator:
    Bertagnolli, Anthony D.
    Abstract:
    Continental shelves located along eastern boundary currents occupy relatively small volumes of the world’s oceans, yet are responsible for a large proportion of global primary production. The Oregon coast is among these ecosystems. Recent analyses of dissolved oxygen at shallow depths in the water column has suggested increasing episodes of...
    Resource Type:
    Dissertation
    Full Text:
    .................................................................................................122 Appendix C: Functional genes observed in transcriptomes displaying significant increases under

  • Proteomic analyses of marine bacteria

    Creator:
    Sowell, Sarah M.
    Abstract:
    Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
    Resource Type:
    Dissertation
    Full Text:
    Accurate Mass and Time (AMT) tag library………………………………………………… 163 C. Relative quantification of Cand

  • Prokaryotes associated with marine crust

    Creator:
    Mason, Olivia Underwood
    Abstract:
    Oceanic crust covers nearly 70% of the Earth's surface, of which, the upper, sediment layer is estimated to harbor substantial microbial biomass. Marine crust; however, extends several kilometers beyond this surficial layer, and includes the basalt and gabbro layers. In particular, the basalt layer has high permeabilities which allows for...
    Resource Type:
    Dissertation
    Full Text:
    affiliations of basalt sequences from bacterial OCC ..... 119 C. Summary of phylogenetic affiliations of

  • A genomic and proteomic characterization of the first cultured oligotrophic marine Gammaproteobacterium from the SAR92 clade

    Creator:
    Mellbye, Brett L.
    Abstract:
    High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain...
    Resource Type:
    Honors College Thesis
    Full Text:
    2390 open reading frames. The G+C content is 49.10 mol %. The bacterium possesses all major metabolic